Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Regulation by aldosterone of Na+,K+-ATPase mRNAs, protein synthesis, and sodium transport in cultured kidney cells.
Verrey F
,
Schaerer E
,
Zoerkler P
,
Paccolat MP
,
Geering K
,
Kraehenbuhl JP
,
Rossier BC
.
???displayArticle.abstract???
Transepithelial Na+ reabsorption across tight epithelia is regulated by aldosterone. Mineralocorticoids modulate the expression of a number of proteins. Na+,K+-ATPase has been identified as an aldosterone-induced protein (Geering, K., M. Girardet, C. Bron, J. P. Kraehenbuhl, and B. C. Rossier, 1982, J. Biol. Chem., 257:10338-10343). Using A6 cells (kidney of Xenopus laevis) grown on filters we demonstrated by Northern blot analysis that the induction of Na+,K+-ATPase was mainly mediated by a two- to fourfold accumulation of both alpha- and beta-subunit mRNAs. The specific competitor spironolactone decreased basal Na+ transport, Na+,K+-ATPase mRNA, and the relative rate of protein biosynthesis, and it blocked the response to aldosterone. Cycloheximide inhibited the aldosterone-dependent sodium transport but did not significantly affect the cytoplasmic accumulation of Na+,K+-ATPase mRNA induced by aldosterone.
Alonso,
Comparison of three actin-coding sequences in the mouse; evolutionary relationships between the actin genes of warm-blooded vertebrates.
1986, Pubmed
Alonso,
Comparison of three actin-coding sequences in the mouse; evolutionary relationships between the actin genes of warm-blooded vertebrates.
1986,
Pubmed
Corvol,
Mechanism of the antimineralocorticoid effects of spirolactones.
1981,
Pubmed
Elder,
Specific stimulation of actin gene transcription by epidermal growth factor and cycloheximide.
1984,
Pubmed
Fanestil,
On the mechanism of action of aldosterone on sodium transport: effects of inhibitors of RNA and of protein synthesis.
1966,
Pubmed
Firzlaff,
Dexamethasone increases the number of RNA polymerase II molecules transcribing integrated mouse mammary tumor virus DNA and flanking mouse sequences.
1984,
Pubmed
Geering,
Hormonal regulation of (Na+,K+)-ATPase biosynthesis in the toad bladder. Effect of aldosterone and 3,5,3'-triiodo-L-thyronine.
1982,
Pubmed
Geering,
Receptor occupancy vs. induction of Na+-K+-ATPase and Na+ transport by aldosterone.
1985,
Pubmed
Geering,
Membrane insertion of alpha- and beta-subunits of Na+,K+-ATPase.
1985,
Pubmed
,
Xenbase
Girardet,
Immunochemical evidence for a transmembrane orientation of both the (Na+, K+)-ATPase subunits.
1981,
Pubmed
Handler,
Use of cultured epithelia to study transport and its regulation.
1983,
Pubmed
Hayward,
Activation of vitellogenin gene transcription is a direct response to estrogen in Xenopus laevis liver.
1982,
Pubmed
,
Xenbase
Ivarie,
Interaction of thyroid and glucocorticoid hormones in rat pituitary tumor cells. Specificity and diversity of the responses analyzed by two-dimensional gel electrophoresis.
1981,
Pubmed
Kawakami,
Primary structure of the alpha-subunit of Torpedo californica (Na+ + K+)ATPase deduced from cDNA sequence.
,
Pubmed
Kawakami,
Molecular cloning and sequence analysis of human Na,K-ATPase beta-subunit.
1986,
Pubmed
Kawakami,
Primary structure of the alpha-subunit of human Na,K-ATPase deduced from cDNA sequence.
1986,
Pubmed
LOWRY,
Protein measurement with the Folin phenol reagent.
1951,
Pubmed
McKnight,
The induction of ovalbumin and conalbumin mRNA by estrogen and progesterone in chick oviduct explant cultures.
1978,
Pubmed
McKnight,
Butyrate and related inhibitors of histone deacetylation block the induction of egg white genes by steroid hormones.
1980,
Pubmed
Mohun,
Cell type-specific activation of actin genes in the early amphibian embryo.
,
Pubmed
,
Xenbase
Noguchi,
Primary structure of the beta-subunit of Torpedo californica (Na+ + K+)-ATPase deduced from the cDNA sequence.
1986,
Pubmed
Ringold,
Glucocorticoid-stimulated accumulation of mouse mammary tumor virus RNA: increased rate of synthesis of viral RNA.
1977,
Pubmed
Ringold,
Dexamethasone-mediated induction of mouse mammary tumor virus RNA: a system for studying glucocorticoid action.
1975,
Pubmed
Ringold,
Inhibition of protein synthesis stimulates the transcription of human beta-interferon genes in Chinese hamster ovary cells.
1984,
Pubmed
Rose,
Expression from cloned cDNA of cell-surface secreted forms of the glycoprotein of vesicular stomatitis virus in eucaryotic cells.
1982,
Pubmed
Rossier,
Role of RNA in the action of aldosterone on Na+ transport.
1978,
Pubmed
Sariban-Sohraby,
Apical sodium uptake in toad kidney epithelial cell line A6.
1983,
Pubmed
Schibler,
Tissue-specific expression of mouse alpha-amylase genes.
1980,
Pubmed
Schneider,
Molecular cloning of rat brain Na,K-ATPase alpha-subunit cDNA.
1985,
Pubmed
Shull,
Amino-acid sequence of the catalytic subunit of the (Na+ + K+)ATPase deduced from a complementary DNA.
,
Pubmed
Shull,
Amino-acid sequence of the beta-subunit of the (Na+ + K+)ATPase deduced from a cDNA.
,
Pubmed
Steele,
Porous-bottom dishes for culture of polarized cells.
1986,
Pubmed
Thomas,
Hybridization of denatured RNA transferred or dotted nitrocellulose paper.
1983,
Pubmed
,
Xenbase
Truscello,
Effects of butyrate on histone deacetylation and aldosterone-dependent Na+ transport in the toad bladder.
1983,
Pubmed
Truscello,
Thyroid hormone antagonizes an aldosterone-induced protein: a candidate mediator for the late mineralocorticoid response.
1986,
Pubmed
Vandekerckhove,
Diversity of expression of non-muscle actin in amphibia.
1981,
Pubmed
,
Xenbase
Watlington,
Aldosterone and corticosterone binding and effects on Na+ transport in cultured kidney cells.
1982,
Pubmed
,
Xenbase
Wilce,
Actions of aldosterone on polyadenylated ribonucleic acid and Na+ transport in the toad bladder.
1976,
Pubmed
