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We have determined the sequence of cloned cDNAs derived from a 1,665-nucleotide mRNA which transiently accumulates during Xenopus laevis embryogenesis. Computer analysis of the deduced amino acid sequence revealed that this mRNA encodes a 47-kilodalton type I intermediate filament subunit, i.e., a cytokeratin. As is common to all intermediate filament subunits so far examined, the predicted polypeptide, named XK70, contains N- and C-terminal domains flanking a central alpha-helical rod domain. The overall amino acid homology between XK70 and a human 50-kilodalton type I keratin is 47%; homology within the alpha-helical domain is 57%. The N-terminal domain, which is not completely contained in our cDNAs, is basic, contains 42% serine plus alanine, and includes five copies of a six-amino-acid repeating unit. The C-terminal domain has a high alpha-helical content and contains a region with sequence homology to the C-terminal domains of other type I and type III intermediate filament proteins. We suggest that different keratin filament subtypes may have different functional roles during amphibian oogenesis and embryogenesis.
Bailey,
Methylmercury as a reversible denaturing agent for agarose gel electrophoresis.
1976, Pubmed
Bailey,
Methylmercury as a reversible denaturing agent for agarose gel electrophoresis.
1976,
Pubmed
Banks-Schlegel,
Keratin alterations during embryonic epidermal differentiation: a presage of adult epidermal maturation.
1982,
Pubmed
Brosius,
Gene organization and primary structure of a ribosomal RNA operon from Escherichia coli.
1981,
Pubmed
Chou,
Conformational parameters for amino acids in helical, beta-sheet, and random coil regions calculated from proteins.
1974,
Pubmed
Chou,
Prediction of protein conformation.
1974,
Pubmed
Church,
Genomic sequencing.
1984,
Pubmed
Fitzgerald,
The sequence 5'-AAUAAA-3'forms parts of the recognition site for polyadenylation of late SV40 mRNAs.
1981,
Pubmed
Franz,
Intermediate-size filaments in a germ cell: Expression of cytokeratins in oocytes and eggs of the frog Xenopus.
1983,
Pubmed
,
Xenbase
Garnier,
Analysis of the accuracy and implications of simple methods for predicting the secondary structure of globular proteins.
1978,
Pubmed
Geisler,
Comparison of the proteins of two immunologically distinct intermediate-sized filaments by amino acid sequence analysis: desmin and vimentin.
1981,
Pubmed
Geisler,
Proteinchemical characterization of three structurally distinct domains along the protofilament unit of desmin 10 nm filaments.
1982,
Pubmed
Geisler,
The amino acid sequence of chicken muscle desmin provides a common structural model for intermediate filament proteins.
1982,
Pubmed
Gibbs,
The diagram, a method for comparing sequences. Its use with amino acid and nucleotide sequences.
1970,
Pubmed
Godsave,
Intermediate filaments in the Xenopus oocyte: the appearance and distribution of cytokeratin-containing filaments.
1984,
Pubmed
,
Xenbase
Hanukoglu,
The cDNA sequence of a human epidermal keratin: divergence of sequence but conservation of structure among intermediate filament proteins.
1982,
Pubmed
Hanukoglu,
The cDNA sequence of a Type II cytoskeletal keratin reveals constant and variable structural domains among keratins.
1983,
Pubmed
Hoffmann,
Amino acid sequence of the carboxy-terminal part of an acidic type I cytokeratin of molecular weight 51 000 from Xenopus laevis epidermis as predicted from the cDNA sequence.
1984,
Pubmed
,
Xenbase
Johnson,
Structure of a gene for the human epidermal 67-kDa keratin.
1985,
Pubmed
Jorcano,
Identification of two types of keratin polypeptides within the acidic cytokeratin subfamily I.
1984,
Pubmed
Jorcano,
Cell type-specific expression of bovine keratin genes as demonstrated by the use of complementary DNA clones.
1984,
Pubmed
Kim,
Tissue specificity of epithelial keratins: differential expression of mRNAs from two multigene families.
1983,
Pubmed
Lazarides,
Intermediate filaments: a chemically heterogeneous, developmentally regulated class of proteins.
1982,
Pubmed
Lewis,
Sequence of a cDNA clone encoding mouse glial fibrillary acidic protein: structural conservation of intermediate filaments.
1984,
Pubmed
Lipman,
Rapid and sensitive protein similarity searches.
1985,
Pubmed
Marchuk,
Complete sequence of a gene encoding a human type I keratin: sequences homologous to enhancer elements in the regulatory region of the gene.
1985,
Pubmed
Marchuk,
Remarkable conservation of structure among intermediate filament genes.
1984,
Pubmed
Maxam,
Sequencing end-labeled DNA with base-specific chemical cleavages.
1980,
Pubmed
McLachlan,
The 14-fold periodicity in alpha-tropomyosin and the interaction with actin.
1976,
Pubmed
Moll,
Changes in the pattern of cytokeratin polypeptides in epidermis and hair follicles during skin development in human fetuses.
1982,
Pubmed
Moll,
The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells.
1982,
Pubmed
Mount,
A catalogue of splice junction sequences.
1982,
Pubmed
Nelson,
Proteolysis of vimentin and desmin by the Ca2+-activated proteinase specific for these intermediate filament proteins.
1983,
Pubmed
Okayama,
A cDNA cloning vector that permits expression of cDNA inserts in mammalian cells.
1983,
Pubmed
Parry,
Structure of alpha-keratin: structural implication of the amino acid sequences of the type I and type II chain segments.
1977,
Pubmed
Parry,
Analysis of the primary sequence of alpha-tropomyosin from rabbit skeletal muscle.
1975,
Pubmed
Proudfoot,
3' non-coding region sequences in eukaryotic messenger RNA.
1976,
Pubmed
Quax,
Intermediate filament cDNAs from BHK-21 cells: demonstration of distinct genes for desmin and vimentin in all vertebrate classes.
1984,
Pubmed
Quax-Jeuken,
Primary and secondary structure of hamster vimentin predicted from the nucleotide sequence.
1983,
Pubmed
Sagata,
A study on the steady-state population of poly(A)+RNA during early development of Xenopus laevis.
1980,
Pubmed
,
Xenbase
Salim,
Nucleotide sequence of Xenopus laevis 18S ribosomal RNA inferred from gene sequence.
1981,
Pubmed
,
Xenbase
Sargent,
Differential gene expression in the gastrula of Xenopus laevis.
1983,
Pubmed
,
Xenbase
Slack,
Regional biosynthetic markers in the early amphibian embryo.
1984,
Pubmed
Steinert,
The complete cDNA and deduced amino acid sequence of a type II mouse epidermal keratin of 60,000 Da: analysis of sequence differences between type I and type II keratins.
1984,
Pubmed
Steinert,
Complete amino acid sequence of a mouse epidermal keratin subunit and implications for the structure of intermediate filaments.
1983,
Pubmed
Steinert,
Structure of the three-chain unit of the bovine epidermal keratin filament.
1978,
Pubmed
