Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Biosynthesis and secretion of catalytically active acetylcholinesterase in Xenopus oocytes microinjected with mRNA from rat brain and from Torpedo electric organ.
???displayArticle.abstract???
A novel technique was developed for monitoring the level of the mRNA species that direct the synthesis of acetylcholinesterase (AcChoEase; acetylcholine acetylhydrolase, EC 3.1.1.7), using microinjected Xenopus oocytes as a translation system. When injected with poly(A)-containing RNA from whole rat brain or rat cerebellum and from electric organ of Torpedo ocellata, Xenopus oocytes synthesize and secrete catalytically active cholinesterase. The newly synthesized enzyme, which is mostly secreted into the oocytes incubation medium, appears to be primarily AcChoEase because it is inhibited by the specific inhibitor BW 284C51. The new enzymatic activity can be detected after injection of as little as 12.5 ng of poly(A)-containing RNA per oocyte, and there is a linear dependence of the oocytes' ability to form AcChoEase on the amount of injected RNA. The AcChoEase mRNA displays a tau 1/2 of about 10 +/- 3 hr in injected oocytes. The abundance of AcChoEase mRNA in the total nonfractionated mRNA injected was calculated to be ca. 1 x 10(-5), a value similar to the level of AcChoEase protein determined in rat brain. The combination of the high turnover number of AcChoEase, the efficiency of the oocyte system, and the sensitivity of the assay used thus permit the accurate monitoring of the scarce mRNA species that direct the synthesis of this enzyme.
Allende,
The degradation of ribonucleic acids injected into Xenopus laevis oocytes.
1974, Pubmed,
Xenbase
Allende,
The degradation of ribonucleic acids injected into Xenopus laevis oocytes.
1974,
Pubmed
,
Xenbase
AUSTIN,
Two selective inhibitors of cholinesterase.
1953,
Pubmed
Bennett,
Acetylcholinesterase activity and weight measures in fifteen brain areas from six lines of rats.
1966,
Pubmed
Betz,
Evolution of cholinergic proteins in developing slow and fast skeletal muscles in chick embryo.
1980,
Pubmed
Blobel,
Transfer of proteins across membranes. I. Presence of proteolytically processed and unprocessed nascent immunoglobulin light chains on membrane-bound ribosomes of murine myeloma.
1975,
Pubmed
Bon,
Collagen-tailed and hydrophobic components of acetylcholinesterase in Torpedo marmorata electric organ.
1980,
Pubmed
Chan,
Translation of ovalbumin mRNA in Xenopus laevis oocytes. Characterization of the system and effects of estrogen on injected mRNA populations.
1976,
Pubmed
,
Xenbase
Colman,
Export of proteins from oocytes of Xenopus laevis.
1979,
Pubmed
,
Xenbase
Dumont,
Oogenesis in Xenopus laevis (Daudin). I. Stages of oocyte development in laboratory maintained animals.
1972,
Pubmed
,
Xenbase
Fernandez,
Neurotrophic control of 16S acetylcholinesterase at the vertebrate neuromuscular junction.
1979,
Pubmed
Ghysdael,
Frog oocytes synthesize and completely process the precursor polypeptide to virion structural proteins after microinjection of avian myeloblastosis virus RNA.
1977,
Pubmed
,
Xenbase
Gisiger,
A specific form of acetylcholinesterase is secreted by rat sympathetic ganglia.
1977,
Pubmed
Gurdon,
Use of frog eggs and oocytes for the study of messenger RNA and its translation in living cells.
1971,
Pubmed
,
Xenbase
Hall,
Multiple forms of acetylcholinesterase and their distribution in endplate and non-endplate regions of rat diaphragm muscle.
1973,
Pubmed
Jedrzejczyk,
Molecular forms of the cholinesterases inside and outside muscle endplates.
1981,
Pubmed
Johnson,
A rapid, simple radiometric assay for cholinesterase, suitable for multiple determinations.
1975,
Pubmed
Kaplan,
Sequence complexity of polyadenylated RNA obtained from rat brain regions and cultured rat cells of neural origin.
1978,
Pubmed
Karlin,
Chemical distinctions between acetylcholinesterase and the acetylcholine receptor.
1967,
Pubmed
Kimhi,
Acetylcholinesterase in mouse neuroblastoma cells: intracellular and released enzyme.
1980,
Pubmed
Koenig,
Neural induction of the 16S acetylcholinesterase in muscle cell cultures.
1978,
Pubmed
Kusano,
Acetylcholine receptors in the oocyte membrane.
,
Pubmed
,
Xenbase
Labarca,
MRNA-directed synthesis of catalytically active mouse beta-glucuronidase in Xenopus oocytes.
1977,
Pubmed
,
Xenbase
Lai,
Brain regional distribution of glutamic acid decarboxylase, choline acetyltransferase, and acetylcholinesterase in the rat: effects of chronic manganese chloride administration after two years.
1981,
Pubmed
Lanks,
Increased rate of acetylcholinesterase synthesis in differentiating neuroblastoma cells.
1974,
Pubmed
Laskey,
Protein synthesis in oocytes of Xenopus laevis is not regulated by the supply of messenger RNA.
1977,
Pubmed
,
Xenbase
Littauer,
The regulatory function of poly(A) and adjacent 3' sequences in translated RNA.
1982,
Pubmed
Lømo,
Control of junctional acetylcholinesterase by neural and muscular influences in the rat.
1980,
Pubmed
Meedel,
Development of acetylchilinesterase during embryogenesis of the ascidian Ciona intestinalis.
1979,
Pubmed
Miskin,
Microinjected Xenopus oocytes synthesize active human plasminogen activator.
1981,
Pubmed
,
Xenbase
Mohun,
The secretion of proteins in vitro from Xenopus oocytes and their accessory cells: a biochemical and morphological study.
1981,
Pubmed
,
Xenbase
Nudel,
Globin mRNA species containing poly(A) segments of different lengths. Their functional stability in Xenopus oocytes.
1976,
Pubmed
,
Xenbase
Prives,
Appearance and disappearance of acetycholine receptor during differentiation of chick skeletal muscle in vitro.
1976,
Pubmed
Rieger,
Molecular forms of acetylcholinesterase: their de novo synthesis in mouse neuroblastoma cells.
1976,
Pubmed
Rotundo,
Synthesis, transport and fate of acetylcholinesterase in cultured chick embryos muscle cells.
1980,
Pubmed
Rubin,
Regulation of acetylcholinesterase appearance at neuromuscular junctions in vitro.
1980,
Pubmed
Shulman,
Interferon-dependent induction of mRNA activity for (2'-5')oligo-isoadenylate synthetase.
1980,
Pubmed
,
Xenbase
Silman,
Parallel regulation of acetylcholinesterase and pseudocholinesterase in normal, denervated and dystrophic chicken skeletal muscle.
1979,
Pubmed
Soreq,
Secreted proteins in the medium of microinjected Xenopus oocytes are degraded by oocyte proteases.
1981,
Pubmed
,
Xenbase
Soreq,
In vitro translation of polyadenylic acid-free rabbit globin messenger RNA.
1974,
Pubmed
Soreq,
Rate of synthesis and concentration of specific mRNA sequences in cultured Chinese hamster ovary cells compared to liver cells.
1980,
Pubmed
Soreq,
Translational activity and functional stability of human fibroblast beta 1 and beta 2 interferon mRNAs lacking 3'-terminal RNA sequences.
1981,
Pubmed
,
Xenbase
Trevor,
Acetylcholinesterases.
1978,
Pubmed
Valle,
Synthesis and secretion of mouse immunoglobulin chains from Xenopus oocytes.
1981,
Pubmed
,
Xenbase
Vigny,
Active-site catalytic efficiency of acetylcholinesterase molecular forms in Electrophorus, torpedo, rat and chicken.
1978,
Pubmed
Vijayan,
Polyacrylamide gel electrophoresis of rat brain acetylcholinesterase: isoenzymes of normal rat brain.
1974,
Pubmed
Walker,
Regulation of acetylcholinesterase in chick muscle cultures after treatment with diisopropylphosphorofluoridate: ribonucleic acid and protein synthesis.
1976,
Pubmed
Wilson,
Regulation of newly synthesized acetylcholinesterase in muscle cultures treated with diisopropylfluorophosphate.
1974,
Pubmed
