Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Biophys J
2006 Aug 15;914:1471-9. doi: 10.1529/biophysj.106.086421.
Show Gene links
Show Anatomy links
H+ -pumping rhodopsin from the marine alga Acetabularia.
Tsunoda SP
,
Ewers D
,
Gazzarrini S
,
Moroni A
,
Gradmann D
,
Hegemann P
.
???displayArticle.abstract???
An opsin-encoding cDNA was cloned from the marine alga Acetabularia acetabulum. The cDNA was expressed in Xenopus oocytes into functional Acetabularia rhodopsin (AR) mediating H+ carried outward photocurrents of up to 1.2 microA with an action spectrum maximum at 518 nm (AR518). AR is the first ion-pumping rhodopsin found in a plant organism. Steady-state photocurrents of AR are always positive and rise sigmoidally from negative to positive transmembrane voltages. Numerous kinetic details (amplitudes and time constants), including voltage-dependent recovery of the dark state after light-off, are documented with respect to their sensitivities to light, internal and external pH, and the transmembrane voltage. The results are analyzed by enzyme kinetic formalisms using a simplified version of the known photocycle of bacteriorhodopsin (BR). Blue-light causes a shunt of the photocycle under H+ reuptake from the extracellular side. Similarities and differences of AR with BR are pointed out. This detailed electrophysiological characterization highlights voltage dependencies in catalytic membrane processes of this eukaryotic, H+ -pumping rhodopsin and of microbial-type rhodopsins in general.
Béjà,
Bacterial rhodopsin: evidence for a new type of phototrophy in the sea.
2000, Pubmed
Béjà,
Bacterial rhodopsin: evidence for a new type of phototrophy in the sea.
2000,
Pubmed
Bieszke,
The nop-1 gene of Neurospora crassa encodes a seven transmembrane helix retinal-binding protein homologous to archaeal rhodopsins.
1999,
Pubmed
Bieszke,
A eukaryotic protein, NOP-1, binds retinal to form an archaeal rhodopsin-like photochemically reactive pigment.
1999,
Pubmed
Brown,
Connectivity of the retinal Schiff base to Asp85 and Asp96 during the bacteriorhodopsin photocycle: the local-access model.
1998,
Pubmed
Friedrich,
Proteorhodopsin is a light-driven proton pump with variable vectoriality.
2002,
Pubmed
,
Xenbase
Garczarek,
Proton binding within a membrane protein by a protonated water cluster.
2005,
Pubmed
Geibel,
The voltage-dependent proton pumping in bacteriorhodopsin is characterized by optoelectric behavior.
2001,
Pubmed
,
Xenbase
Gergely,
Study of the photocycle and charge motions of the bacteriorhodopsin mutant D96N.
1993,
Pubmed
Grygorczyk,
Measurement of erythroid band 3 protein-mediated anion transport in mRNA-injected oocytes of Xenopus laevis.
1989,
Pubmed
,
Xenbase
Henry,
Comparison of ESTs from juvenile and adult phases of the giant unicellular green alga Acetabularia acetabulum.
2004,
Pubmed
Ikeda,
A Cl(-)-translocating adenosinetriphosphatase in Acetabularia acetabulum. 1. Purification and characterization of a novel type of adenosinetriphosphatase that differs from chloroplast F1 adenosinetriphosphatase.
1990,
Pubmed
Kammann,
Rapid insertional mutagenesis of DNA by polymerase chain reaction (PCR).
1989,
Pubmed
Luecke,
Structural changes in bacteriorhodopsin during ion transport at 2 angstrom resolution.
1999,
Pubmed
Nagel,
Channelrhodopsin-2, a directly light-gated cation-selective membrane channel.
2003,
Pubmed
,
Xenbase
Nagel,
Channelrhodopsin-1: a light-gated proton channel in green algae.
2002,
Pubmed
,
Xenbase
Richter,
Relationship of retinal configuration and internal proton transfer at the end of the bacteriorhodopsin photocycle.
1996,
Pubmed
Schilde,
[Rapid photoelectric effect in the alga Acetabularia].
1968,
Pubmed
Schneider,
Strong homology between the small subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase of two species of Acetabularia and the occurrence of unusual codon usage.
1989,
Pubmed
Smahel,
Vanadate-sensitive ATPase in the plasmalemma of Acetabularia: biochemical and kinetic characterization.
1992,
Pubmed
Stewart,
Regulation of AE2 anion exchanger by intracellular pH: critical regions of the NH(2)-terminal cytoplasmic domain.
2001,
Pubmed
,
Xenbase
Tóth-Boconádi,
Late events in the photocycle of bacteriorhodopsin mutant L93A.
2003,
Pubmed
Tóth-Boconádi,
Photoexcitation of the O-intermediate in bacteriorhodopsin mutant L93A.
2003,
Pubmed
Tyagi,
A new approach to 'megaprimer' polymerase chain reaction mutagenesis without an intermediate gel purification step.
2004,
Pubmed
Waschuk,
Leptosphaeria rhodopsin: bacteriorhodopsin-like proton pump from a eukaryote.
2005,
Pubmed
