Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
???displayArticle.abstract???
A model synthetic peptide substrate of the cyclic AMP-dependent protein kinase (ATP:protein phosphotransferase; EC 2.7.1.37), Leu-Arg-Arg-Ala-Ser-Leu-Gly, closely resembling the local phosphorylation site sequence in porcine hepatic pyruvate kinase, was shown to be phosphorylated in vivo after microinjection into Xenopus oocytes. This result demonstrates that the microinjection technique, utilizing a synthetic peptide substrate, or possibly a synthetic substrate analog inhibitor [Kemp, B. E., Benjamini, E. & Krebs, E. G. (1976) Proc. Natl. Acad. Sci. USA 73, 1038--1042], can be used to study protein phosphorylation-dephosphorylation reactions in living oocytes. This follows, since it is clear that the injected peptide was accessible to the cellular compartment containing the protein kinase.
Bonner,
Protein migration into nuclei. II. Frog oocyte nuclei accumulate a class of microinjected oocyte nuclear proteins and exclude a class of microinjected oocyte cytoplasmic proteins.
1975, Pubmed,
Xenbase
Bonner,
Protein migration into nuclei. II. Frog oocyte nuclei accumulate a class of microinjected oocyte nuclear proteins and exclude a class of microinjected oocyte cytoplasmic proteins.
1975,
Pubmed
,
Xenbase
Bonner,
Protein migration into nuclei. I. Frog oocyte nuclei in vivo accumulate microinjected histones, allow entry to small proteins, and exclude large proteins.
1975,
Pubmed
,
Xenbase
Castagna,
Nuclear protein-kinase activity in perfused rat liver stimulated with dibutyryl-adenosine cyclic 3':5'-monophosphate.
1975,
Pubmed
Daile,
Peptides from myelin basic protein as substrates for adenosine 3', 5'-cyclic monophosphate-dependent protein kinases.
1974,
Pubmed
Daile,
Synthetic substrate for cyclic AMP-dependent protein kinase.
1975,
Pubmed
Feldherr,
The uptake of endogenous proteins by oocyte nuclei.
1975,
Pubmed
,
Xenbase
Glynn,
A simple method for the preparation of 32-P-labelled adenosine triphosphate of high specific activity.
1964,
Pubmed
Guerrier,
[Inhibition of the reinitiation of meiosis in Xenopus laevis oocytes by three natural antiproteases: antipain, chymostatin, and leupeptin].
1977,
Pubmed
,
Xenbase
Gurdon,
Changes in somatic cell nuclei inserted into growing and maturing amphibian oocytes.
1968,
Pubmed
,
Xenbase
Humble,
Non-dependence on native structure of pig liver pyruvate kinase when used as a substrate for cyclic 3',5'-AMP-stimulated protein kinase.
1975,
Pubmed
Jungmann,
Mechanism of action of gonadotropin. IV. Cyclic adenosine monophosphate-dependent translocation of ovarian cytoplasmic cyclic adenosine monophosphate-binding protein and protein kinase to nuclear acceptor sites.
1974,
Pubmed
Kemp,
Substrate specificity of the cyclic AMP-dependent protein kinase.
1975,
Pubmed
Kemp,
Synthetic hexapeptide substrates and inhibitors of 3':5'-cyclic AMP-dependent protein kinase.
1976,
Pubmed
Korenman,
Protein kinase translocation as an early event in the hormonal control of uterine contraction.
1974,
Pubmed
Kuo,
An adenosine 3',5'-monophosphate-dependent protein kinase from Escherichia coli.
1969,
Pubmed
Lincoln,
Adenosine 3':5'-cyclic monophosphate- and guanosine 3':5'-cyclic monophosphate-dependent protein kinases: possible homologous proteins.
1977,
Pubmed
Maller,
Progesterone-stimulated meiotic cell division in Xenopus oocytes. Induction by regulatory subunit and inhibition by catalytic subunit of adenosine 3':5'-monophosphate-dependent protein kinase.
1977,
Pubmed
,
Xenbase
Maller,
Changes in protein phosphorylation accompanying maturation of Xenopus laevis oocytes.
1977,
Pubmed
,
Xenbase
Masui,
Relative roles of the pituitary, follicle cells, and progesterone in the induction of oocyte maturation in Rana pipiens.
1967,
Pubmed
Merriam,
Movement of cytoplasmic proteins into nuclei induced to enlarge and initiate DNA or RNA synthesis.
1969,
Pubmed
POSNER,
EFFECTS OF ELECTRICAL STIMULATION AND EPINEPHRINE ON MUSCLE PHOSPHORYLASE, PHOSPHORYLASE B KINASE, AND ADENOSINE 3',5'-PHOSPHATE.
1965,
Pubmed
Reimann,
Purification and properties of rabbit skeletal muscle adenosine 3',5'-monophosphate-dependent protein kinases.
1971,
Pubmed
Vitto,
Maturation of Xenopus oocytes. I. Facilitation by ouabain.
1976,
Pubmed
,
Xenbase
Wallace,
Protein incorporation by isolated amphibian oocytes. 3. Optimum incubation conditions.
1973,
Pubmed
,
Xenbase
Walsh,
An adenosine 3',5'-monophosphate-dependant protein kinase from rabbit skeletal muscle.
1968,
Pubmed
Zetterqvist,
The minimum substrate of cyclic AMP-stimulated protein kinase, as studied by synthetic peptides representing the phosphorylatable site of pyruvate kinase (type L) of rat liver.
1976,
Pubmed
