Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Studies on the biosynthesis, assembly and secretion of vitellogenin, an oestrogen-induced multicomponent protein.
Penning TM
,
Merry AH
,
Munday KA
,
Akhtar M
.
???displayArticle.abstract???
1. The process by which the egg-yolk protein precursor vitellogenin is biosynthesized, assembled and secreted by Xenopus laevis (South African clawed toad) liver was studied. It was previously shown in other laboratories that vitellogenin contains the two egg-yolk proteins lipovitellin (mol.wt. 140 000) and phosvitin (mol.wt. 35 000). 2. Evidence is presented which shows that Xenopus liver microsomal fractions synthesize precursors of vitellogenin. These precursors were solubilized from the membranes with detergent and analysed by sodium dodecyl sulphate/polyacrylamide-gel electrophoresis. This analysis indicated that there is only one precursor polypeptide, and this has mol.wt. approx. 200 000 +/- 20 000. This demonstrates that the egg-yolk proteins are translated as part of this larger polypeptide. 3. Experiments also demonstrate the existence of a microsomal proteinase which is able to cleave the precursor into smaller fragments. The nature of these fragments provided some indirect evidence that phosvitin and lipovitellin light chains are situated together within the precursor molecule. 4. These precursor data fit in well with structural studies on serum vitellogenin, since it has been shown that the latter protein consists of two identical subunits each with a mobility on sodium dodecyl sulphate/polyacrylamide gels identical with that shown by the microsomal precursor. This indicates that both the intracellular precursor and subunit of vitellogenin have similar (but not necessarily identical) molecular weights. 5. It was also shown that trypsin or chymotrypsin can cleave the serum vitellogenin into leucine- and serine-rich fragments which resemble lipovitellin and phosvitin respectively. Attention is, however, drawn to the fact that the serine-rich fragment is not identical with phosvitin, since it contains eight times more leucine than that expected for the authentic phosvitin molecule [Penning (1976) Ph.D. Thesis, University of Southampton].
Ansari,
Chemical composition of an oestrogen-induced calcium-binding glycolipophosphoprotein in Xenopus laevis.
1971, Pubmed,
Xenbase
Ansari,
Chemical composition of an oestrogen-induced calcium-binding glycolipophosphoprotein in Xenopus laevis.
1971,
Pubmed
,
Xenbase
Bergink,
Precursor-product relationship between amphibian vitellogenin and the yolk proteins, lipovitellin and phosvitin.
1974,
Pubmed
,
Xenbase
Berridge,
Characterization of polysomes from Xenopus liver synthesizing vitellogenin and translation of vitellogenin and albumin messenger RNA's in vitro.
1976,
Pubmed
,
Xenbase
Blobel,
Studies on free and membrane-bound ribosomes in rat liver. I. Distribution as related to total cellular RNA.
1967,
Pubmed
Byers,
Interchain disulfide bonds in procollagen are located in a large nontriple-helical COOH-terminal domain.
1975,
Pubmed
Clemens,
Sequential changes in the protein synthetic activity of male Xenopus laevis liver following induction of egg-yolk proteins by Estradiol-17 beta.
1975,
Pubmed
,
Xenbase
Clemens,
The regulation of egg yolk protein synthesis by steroid hormones.
1974,
Pubmed
,
Xenbase
Deeley,
Vitellogenin synthesis in the avian liver. Vitellogenin is the precursor of the egg yolk phosphoproteins.
1975,
Pubmed
De Nayer,
Synthesis of thyroglobulin subunits in oocytes injected with thyroidal ribonucleic acid.
1974,
Pubmed
,
Xenbase
Di Lauro,
In vitro synthesis of a thyroglobulin precursor by porcine thyroid membrane-bound ribosomes in a heterologous cell-free system.
1975,
Pubmed
Dolphin,
Studies on the induction and biosynthesis of vitellogenin, an oestrogen-induced glycolipophosphoprotein.
1971,
Pubmed
,
Xenbase
DULBECCO,
Plaque formation and isolation of pure lines with poliomyelitis viruses.
1954,
Pubmed
Follett,
The vitellogenic response in the South African clawed toad (Xenopus laevis Daudin).
1968,
Pubmed
,
Xenbase
Follett,
The effects of oestrogen and gonadotrophins on lipid and protein metabolism in Xenopus laevis Daudin.
1968,
Pubmed
,
Xenbase
Gasbarro,
A new unified Biuret reagent stabilized with EDNA.
1972,
Pubmed
HOWELL,
CHARACTERIZATION OF RIBOSOMAL AGGREGATES ISOLATED FROM LIVER.
1964,
Pubmed
Jost,
Organization of vitellogenin polysomes, size of the mRNA and polyadenylate fragment.
1976,
Pubmed
Lansman,
Pulse-recovery studies on cycloheximide-insensitive protein synthesis in Neurospora. Association of products with cytochrome-oxidase.
1974,
Pubmed
Lewis,
A rapidly sedimenting fraction of rat liver endoplasmic reticulum.
1973,
Pubmed
LOWRY,
Protein measurement with the Folin phenol reagent.
1951,
Pubmed
Merry,
Studies on the assembly and secretion of a multicomponent protein. The biosynthesis of vitellogenin, an oestrogen-induced glycolipophosphoprotein.
1973,
Pubmed
,
Xenbase
Munday,
Oestrogen-induced calcium-binding protein in Xenopus laevis.
1968,
Pubmed
,
Xenbase
Neville,
Molecular weight determination of protein-dodecyl sulfate complexes by gel electrophoresis in a discontinuous buffer system.
1971,
Pubmed
Redshaw,
The crystalline yolk-platelet proteins and their soluble plasma precursor in an amphibian, Xenopus laevis.
1971,
Pubmed
,
Xenbase
Shapiro,
In vitro translation and estradiol-17beta induction of Xenopus laevis vitellogenin messenger RNA.
1976,
Pubmed
,
Xenbase
Spiro,
Glycoprotein biosynthesis: studies on thyroglobulin. Characterization of a particulate precursor and radioisotope incorporation by thyroid slices and particle systems.
1966,
Pubmed
Spiro,
Subunit heterogeneity of thyroglobulin.
1973,
Pubmed
Tata,
Differential subnuclear distribution of polyadenylate-rich ribonuclei acid during induction of egg-yolk protein synthesis in male Xenopus liver by oestradiol-17 beta.
1975,
Pubmed
,
Xenbase
Vassart,
Translation of thyroglobulin 33S messenger RNA as a means of determining thyroglobulin quaternary structure.
1975,
Pubmed
,
Xenbase
Vecchio,
Biosynthesis of thyroid iodoproteins in vivo and in tissue slices.
1972,
Pubmed
Wallace,
Studies on amphibian yolk. IX. Xenopus vitellogenin.
1970,
Pubmed
,
Xenbase
Wallace,
Studies on amphibian yolk. 8. The estrogen-induced hepatic synthesis of a serum lipophosphoprotein and its selective uptake by the ovary and trasformation into yolk platelet proteins in Xenopus laevis.
1969,
Pubmed
,
Xenbase
Wallace,
Studies on amphibian yolk. VII. Serum phosphoprotein synthesis by vitellogenic females and estrogen-treated males of Xenopus laevis.
1968,
Pubmed
,
Xenbase
Wallace,
Studies on amphibian yolk. X. The relative roles of autosynthetic and heterosynthetic processes during yolk protein assembly by isolated oocytes.
1972,
Pubmed
,
Xenbase
Wallace,
The induced synthesis and transport of yolk proteins and their accumulation by the oocyte in Xenopus laevis.
1968,
Pubmed
,
Xenbase
Weber,
Measurement of molecular weights by electrophoresis on SDS-acrylamide gel.
1972,
Pubmed
