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J Neurosci
2004 Jun 23;2425:5684-93. doi: 10.1523/JNEUROSCI.0492-04.2004.
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A retinal-specific regulator of G-protein signaling interacts with Galpha(o) and accelerates an expressed metabotropic glutamate receptor 6 cascade.
Dhingra A
,
Faurobert E
,
Dascal N
,
Sterling P
,
Vardi N
.
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G(o) is the most abundant G-protein in the brain, but its regulators are essentially unknown. In retina, Galpha(o1) is obligatory in mediating the metabotropic glutamate receptor 6 (mGluR6)-initiated ON response. To identify the interactors of G(o), we conducted a yeast two-hybrid screen with constituitively active Galpha(o) as a bait. The screen frequently identified a regulator of G-protein signaling (RGS), Ret-RGS1, the interaction of which we confirmed by coimmunoprecipitation with Galpha(o) in transfected cells and in retina. Ret-RGS1 localized to the dendritic tips of ON bipolar neurons, along with mGluR6 and Galpha(o1). When Ret-RGS1 was coexpressed in Xenopus oocytes with mGluR6, Galpha(o1), and a GIRK (G-protein-gated inwardly rectifying K+) channel, it accelerated the deactivation of the channel response to glutamate in a concentration-dependent manner. Because light onset suppresses glutamate release from photoreceptors onto the ON bipolar dendrites, Ret-RGS1 should accelerate the rising phase of the light response of the ON bipolar cell. This would tend to match its kinetics to that of the OFF bipolar that arises directly from ligand-gated channels.
Ashmore,
Transmission of visual signals to bipolar cells near absolute threshold.
1979, Pubmed
Ashmore,
Transmission of visual signals to bipolar cells near absolute threshold.
1979,
Pubmed
Barker,
RGSZ1 and Ret RGS: two of several splice variants from the gene RGS20.
2001,
Pubmed
Berghard,
Sensory transduction in vomeronasal neurons: evidence for G alpha o, G alpha i2, and adenylyl cyclase II as major components of a pheromone signaling cascade.
1996,
Pubmed
Berman,
The GTPase-activating protein RGS4 stabilizes the transition state for nucleotide hydrolysis.
1996,
Pubmed
Brandstätter,
Distributions of two homologous synaptic vesicle proteins, synaptoporin and synaptophysin, in the mammalian retina.
1996,
Pubmed
Chen,
Slowed recovery of rod photoresponse in mice lacking the GTPase accelerating protein RGS9-1.
2000,
Pubmed
Chidiac,
Activity, regulation, and intracellular localization of RGS proteins.
2003,
Pubmed
Chun,
Some horizontal cells of the bovine retina receive input synapses in the inner plexiform layer.
1993,
Pubmed
Cohen,
Convergence and divergence of cones onto bipolar cells in the central area of cat retina.
1990,
Pubmed
Cowan,
High expression levels in cones of RGS9, the predominant GTPase accelerating protein of rods.
1998,
Pubmed
Dascal,
Voltage clamp recordings from Xenopus oocytes.
2001,
Pubmed
,
Xenbase
de la Villa,
L-glutamate-induced responses and cGMP-activated channels in three subtypes of retinal bipolar cells dissociated from the cat.
1995,
Pubmed
De Vries,
GAIP is membrane-anchored by palmitoylation and interacts with the activated (GTP-bound) form of G alpha i subunits.
1996,
Pubmed
De Vries,
RGS-GAIP, a GTPase-activating protein for Galphai heterotrimeric G proteins, is located on clathrin-coated vesicles.
1998,
Pubmed
De Vries,
The regulator of G protein signaling family.
2000,
Pubmed
Dhingra,
Light response of retinal ON bipolar cells requires a specific splice variant of Galpha(o).
2002,
Pubmed
Dhingra,
The light response of ON bipolar neurons requires G[alpha]o.
2000,
Pubmed
Dolphin,
Mechanisms of modulation of voltage-dependent calcium channels by G proteins.
1998,
Pubmed
Doupnik,
RGS proteins reconstitute the rapid gating kinetics of gbetagamma-activated inwardly rectifying K+ channels.
1997,
Pubmed
,
Xenbase
Euler,
Glutamate responses of bipolar cells in a slice preparation of the rat retina.
1996,
Pubmed
Faurobert,
The core domain of a new retina specific RGS protein stimulates the GTPase activity of transducin in vitro.
1997,
Pubmed
Faurobert,
RET-RGS, a retina-specific regulator of G-protein signaling, is located in synaptic regions of the rat retina.
1999,
Pubmed
Gomez,
Light transduction in invertebrate hyperpolarizing photoreceptors: possible involvement of a Go-regulated guanylate cyclase.
2000,
Pubmed
Greif,
Altered regulation of potassium and calcium channels by GABA(B) and adenosine receptors in hippocampal neurons from mice lacking Galpha(o).
2000,
Pubmed
Grunwald,
Detection of alpha-transducin in retinal rods but not cones.
1986,
Pubmed
He,
RGS9, a GTPase accelerator for phototransduction.
1998,
Pubmed
Herms,
Evidence of presynaptic location and function of the prion protein.
1999,
Pubmed
Huff,
Physical and immunological characterization of a guanine nucleotide-binding protein purified from bovine cerebral cortex.
1985,
Pubmed
Huttner,
Synapsin I (protein I), a nerve terminal-specific phosphoprotein. III. Its association with synaptic vesicles studied in a highly purified synaptic vesicle preparation.
1983,
Pubmed
Jeong,
Differential regulation of G protein-gated inwardly rectifying K(+) channel kinetics by distinct domains of RGS8.
2001,
Pubmed
Johnson,
Regional differences in GABA and GAD immunoreactivity in rabbit horizontal cells.
1998,
Pubmed
Jordan,
Modulation of rap activity by direct interaction of Galpha(o) with Rap1 GTPase-activating protein.
1999,
Pubmed
Keren-Raifman,
Expression levels of RGS7 and RGS4 proteins determine the mode of regulation of the G protein-activated K(+) channel and control regulation of RGS7 by G beta 5.
2001,
Pubmed
,
Xenbase
Kleuss,
Assignment of G-protein subtypes to specific receptors inducing inhibition of calcium currents.
1991,
Pubmed
Kojima,
A novel Go-mediated phototransduction cascade in scallop visual cells.
1997,
Pubmed
Leaney,
The role of members of the pertussis toxin-sensitive family of G proteins in coupling receptors to the activation of the G protein-gated inwardly rectifying potassium channel.
2000,
Pubmed
Lerea,
Identification of specific transducin alpha subunits in retinal rod and cone photoreceptors.
1986,
Pubmed
Lerea,
Alpha transducin is present in blue-, green-, and red-sensitive cone photoreceptors in the human retina.
1989,
Pubmed
Li,
Maintenance of cellular levels of G-proteins: different efficiencies of alpha s and alpha o synthesis in GH3 cells.
1996,
Pubmed
Luo,
Interaction of heterotrimeric G protein Galphao with Purkinje cell protein-2. Evidence for a novel nucleotide exchange factor.
1999,
Pubmed
Lyubarsky,
RGS9-1 is required for normal inactivation of mouse cone phototransduction.
2001,
Pubmed
Martemyanov,
The DEP domain determines subcellular targeting of the GTPase activating protein RGS9 in vivo.
2003,
Pubmed
Martin,
Spatial density and immunoreactivity of bipolar cells in the macaque monkey retina.
1992,
Pubmed
Mendel,
Participation of the protein Go in multiple aspects of behavior in C. elegans.
1995,
Pubmed
Natochin,
Inhibition of GDP/GTP exchange on G alpha subunits by proteins containing G-protein regulatory motifs.
2001,
Pubmed
Nawy,
The metabotropic receptor mGluR6 may signal through G(o), but not phosphodiesterase, in retinal bipolar cells.
1999,
Pubmed
Nawy,
Suppression by glutamate of cGMP-activated conductance in retinal bipolar cells.
1990,
Pubmed
Nawy,
cGMP-gated conductance in retinal bipolar cells is suppressed by the photoreceptor transmitter.
1991,
Pubmed
Nomura,
Developmentally regulated postsynaptic localization of a metabotropic glutamate receptor in rat rod bipolar cells.
1994,
Pubmed
Ross,
GTPase-activating proteins for heterotrimeric G proteins: regulators of G protein signaling (RGS) and RGS-like proteins.
2000,
Pubmed
Saitoh,
RGS8 accelerates G-protein-mediated modulation of K+ currents.
1997,
Pubmed
,
Xenbase
Sharon,
Positive and negative coupling of the metabotropic glutamate receptors to a G protein-activated K+ channel, GIRK, in Xenopus oocytes.
1997,
Pubmed
,
Xenbase
Shiells,
Properties of the cGMP-activated channel of retinal on-bipolar cells.
1992,
Pubmed
Shiells,
Responses of rod bipolar cells isolated from dogfish retinal slices to concentration-jumps of glutamate.
1994,
Pubmed
Shiells,
Glutamate receptors of rod bipolar cells are linked to a cyclic GMP cascade via a G-protein.
1990,
Pubmed
Sterling,
Architecture of rod and cone circuits to the on-beta ganglion cell.
1988,
Pubmed
Sternweis,
Isolation of two proteins with high affinity for guanine nucleotides from membranes of bovine brain.
1984,
Pubmed
Strettoi,
The organization of the inner nuclear layer of the rabbit retina.
1995,
Pubmed
Tall,
Mammalian Ric-8A (synembryn) is a heterotrimeric Galpha protein guanine nucleotide exchange factor.
2003,
Pubmed
Valenzuela,
G alpha(o) is necessary for muscarinic regulation of Ca2+ channels in mouse heart.
1997,
Pubmed
Vardi,
Identification of a G-protein in depolarizing rod bipolar cells.
1993,
Pubmed
Vardi,
Alpha subunit of Go localizes in the dendritic tips of ON bipolar cells.
1998,
Pubmed
Vardi,
ON cone bipolar cells in rat express the metabotropic receptor mGluR6.
1997,
Pubmed
Vardi,
Localization of mGluR6 to dendrites of ON bipolar cells in primate retina.
2000,
Pubmed
Vorobiov,
Coupling of the muscarinic m2 receptor to G protein-activated K(+) channels via Galpha(z) and a receptor-Galpha(z) fusion protein. Fusion between the receptor and Galpha(z) eliminates catalytic (collision) coupling.
2000,
Pubmed
,
Xenbase
Wang,
RGSZ1, a Gz-selective RGS protein in brain. Structure, membrane association, regulation by Galphaz phosphorylation, and relationship to a Gz gtpase-activating protein subfamily.
1998,
Pubmed
Yakubovich,
Slow modal gating of single G protein-activated K+ channels expressed in Xenopus oocytes.
2000,
Pubmed
,
Xenbase
Zhang,
Gating properties of GIRK channels activated by Galpha(o)- and Galpha(i)-coupled muscarinic m2 receptors in Xenopus oocytes: the role of receptor precoupling in RGS modulation.
2002,
Pubmed
,
Xenbase
Zhang,
GTPase regulators and photoresponses in cones of the eastern chipmunk.
2003,
Pubmed
Zheng,
RGS-PX1, a GAP for GalphaS and sorting nexin in vesicular trafficking.
2001,
Pubmed
