Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Eur J Neurosci
2006 May 01;239:2297-310. doi: 10.1111/j.1460-9568.2006.04734.x.
Show Gene links
Show Anatomy links
Ca1.2 and CaV1.3 neuronal L-type calcium channels: differential targeting and signaling to pCREB.
Zhang H
,
Fu Y
,
Altier C
,
Platzer J
,
Surmeier DJ
,
Bezprozvanny I
.
???displayArticle.abstract???
Neurons express multiple types of voltage-gated calcium (Ca2+) channels. Two subtypes of neuronal L-type Ca2+ channels are encoded by CaV1.2 and CaV1.3 pore-forming subunits. To compare targeting of CaV1.2 and CaV1.3 L-type Ca2+ channels, we transfected rat hippocampal neuronal cultures with surface-epitope-tagged sHA-CaV1.2 or sHA-CaV1.3a constructs and found that: (i) both sHA-CaV1.2 and sHA-CaV1.3a form clusters on the neuronal plasma membrane surface; (ii) when compared with sHA-CaV1.2 surface clusters, the sHA-CaV1.3a surface clusters were 10% larger and 25% brighter, but 35% less abundant; (iii) 81% of sHA-CaV1.2 surface clusters, but only 48% of sHA-CaV1.3a surface clusters, co-localized with synapsin clusters; (iv) co-expression with GFP-Shank-1B had no significant effect on sHA-CaV1.2 surface clusters, but promoted formation and synaptic localization of sHA-CaV1.3a surface clusters. In experiments with dihydropyridine-resistant CaV1.2 and CaV1.3a mutants we demonstrated that CaV1.3a L-type Ca2+ channels preferentially mediate nuclear pCREB signaling in hippocampal neurons at low, but not at high, levels of stimulation. In experiments with primary neuronal cultures from CaV1.3 knockout mice we discovered that CaV1.3 channels play a more important role in pCREB signaling in striatal medium spiny neurons than in hippocampal neurons. Our results provide novel insights into the function of CaV1.2 and CaV1.3 L-type Ca2+ channels in the brain.
Altier,
Trafficking of L-type calcium channels mediated by the postsynaptic scaffolding protein AKAP79.
2002, Pubmed
Altier,
Trafficking of L-type calcium channels mediated by the postsynaptic scaffolding protein AKAP79.
2002,
Pubmed
Bezprozvanny,
Voltage-dependent blockade of diverse types of voltage-gated Ca2+ channels expressed in Xenopus oocytes by the Ca2+ channel antagonist mibefradil (Ro 40-5967).
1995,
Pubmed
,
Xenbase
Bito,
CREB phosphorylation and dephosphorylation: a Ca(2+)- and stimulus duration-dependent switch for hippocampal gene expression.
1996,
Pubmed
Bittinger,
Activation of cAMP response element-mediated gene expression by regulated nuclear transport of TORC proteins.
2004,
Pubmed
Catterall,
Structure and regulation of voltage-gated Ca2+ channels.
2000,
Pubmed
Clark,
Neurological phenotype and synaptic function in mice lacking the CaV1.3 alpha subunit of neuronal L-type voltage-dependent Ca2+ channels.
2003,
Pubmed
Conkright,
TORCs: transducers of regulated CREB activity.
2003,
Pubmed
Davare,
A beta2 adrenergic receptor signaling complex assembled with the Ca2+ channel Cav1.2.
2001,
Pubmed
Deisseroth,
Signaling from synapse to nucleus: the logic behind the mechanisms.
2003,
Pubmed
Dolmetsch,
Signaling to the nucleus by an L-type calcium channel-calmodulin complex through the MAP kinase pathway.
2001,
Pubmed
Dunlap,
Exocytotic Ca2+ channels in mammalian central neurons.
1995,
Pubmed
Graef,
L-type calcium channels and GSK-3 regulate the activity of NF-ATc4 in hippocampal neurons.
1999,
Pubmed
He,
Motif III S5 of L-type calcium channels is involved in the dihydropyridine binding site. A combined radioligand binding and electrophysiological study.
1997,
Pubmed
,
Xenbase
Hell,
Identification and differential subcellular localization of the neuronal class C and class D L-type calcium channel alpha 1 subunits.
1993,
Pubmed
Herlitze,
Targeting mechanisms of high voltage-activated Ca2+ channels.
2003,
Pubmed
Iourgenko,
Identification of a family of cAMP response element-binding protein coactivators by genome-scale functional analysis in mammalian cells.
2003,
Pubmed
Koschak,
alpha 1D (Cav1.3) subunits can form l-type Ca2+ channels activating at negative voltages.
2001,
Pubmed
Kurschner,
Neuronal interleukin-16 (NIL-16): a dual function PDZ domain protein.
1999,
Pubmed
Kurschner,
CIPP, a novel multivalent PDZ domain protein, selectively interacts with Kir4.0 family members, NMDA receptor subunits, neurexins, and neuroligins.
1998,
Pubmed
Lipscombe,
L-type calcium channels: the low down.
2004,
Pubmed
Mangoni,
Functional role of L-type Cav1.3 Ca2+ channels in cardiac pacemaker activity.
2003,
Pubmed
Maximov,
Synaptic targeting of N-type calcium channels in hippocampal neurons.
2002,
Pubmed
,
Xenbase
Namkung,
Requirement for the L-type Ca(2+) channel alpha(1D) subunit in postnatal pancreatic beta cell generation.
2001,
Pubmed
Obermair,
Differential targeting of the L-type Ca2+ channel alpha 1C (CaV1.2) to synaptic and extrasynaptic compartments in hippocampal neurons.
2004,
Pubmed
Olson,
G-protein-coupled receptor modulation of striatal CaV1.3 L-type Ca2+ channels is dependent on a Shank-binding domain.
2005,
Pubmed
Platzer,
Congenital deafness and sinoatrial node dysfunction in mice lacking class D L-type Ca2+ channels.
2000,
Pubmed
Rajadhyaksha,
L-Type Ca(2+) channels are essential for glutamate-mediated CREB phosphorylation and c-fos gene expression in striatal neurons.
1999,
Pubmed
Safa,
Functional properties of Cav1.3 (alpha1D) L-type Ca2+ channel splice variants expressed by rat brain and neuroendocrine GH3 cells.
2001,
Pubmed
Sala,
Inhibition of dendritic spine morphogenesis and synaptic transmission by activity-inducible protein Homer1a.
2003,
Pubmed
Screaton,
The CREB coactivator TORC2 functions as a calcium- and cAMP-sensitive coincidence detector.
2004,
Pubmed
Seisenberger,
Functional embryonic cardiomyocytes after disruption of the L-type alpha1C (Cav1.2) calcium channel gene in the mouse.
2000,
Pubmed
Tang,
Disturbed Ca2+ signaling and apoptosis of medium spiny neurons in Huntington's disease.
2005,
Pubmed
Weick,
Interactions with PDZ proteins are required for L-type calcium channels to activate cAMP response element-binding protein-dependent gene expression.
2003,
Pubmed
Wittemann,
Synaptic localization and presynaptic function of calcium channel beta 4-subunits in cultured hippocampal neurons.
2000,
Pubmed
Xu,
Neuronal Ca(V)1.3alpha(1) L-type channels activate at relatively hyperpolarized membrane potentials and are incompletely inhibited by dihydropyridines.
2001,
Pubmed
,
Xenbase
Zhang,
Functional Roles of Ca(v)1.3 (alpha(1D)) calcium channel in sinoatrial nodes: insight gained using gene-targeted null mutant mice.
2002,
Pubmed
Zhang,
Association of CaV1.3 L-type calcium channels with Shank.
2005,
Pubmed
,
Xenbase
Zhang,
Genome-wide analysis of cAMP-response element binding protein occupancy, phosphorylation, and target gene activation in human tissues.
2005,
Pubmed
