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Estrogen induced gene 121 (EIG121) and EIG121-like (EIG121L) are evolutionarily conserved genes. But, their function is still unknown. Here, we report the expression pattern of Xenopus EIG121-like (xEIG121L) during early development. Its expression was first detected at stage 9 after mid-blastula transition, attained its maximal level at the gastrula stage, and remained constant until the tadpole stage. Whole-mount in situ hybridization revealed that xEIG121L was expressed strongly in the ventralectoderm at the gastrula stage, and in the anteriorectoderm surrounding the neural plate at the neurula stage. xEIG121L expression was especially high in the presumptive hatching gland and cement gland regions in the neurula. At the tailbud stage, xEIG121L expression was limited to the hatching gland; an inverted Y type staining, characteristic of the hatching gland, was observed. However, at the tadpole stage, xEIG121L was expressed broadly in the head, heart and fin.
Fig. 3. Spatial expression of xEIG121L in blastula and gastrula embryos. Whole-mount in situ hybridization was performed on stage 9 (A and B), stage 10.5 (C and D), stage 11.5 (Eâ G) and stage 12.5 (Hâ K) embryos. (A, C, E, H) Animal views. (B, D, F ,I) Vegetal views. The blastopore is indicated in each embryo by a dashed line (D, F, I). Dorsal is upward (E, F, H, I). (G) Sagittal section of the stage 11.5 embryo. Two arrowheads indicate the blastopore. Dorsal is to the right. (J) Transverse section of the stage 12.5 embryo. Ventral is to the top. (K) Magnified view of (J).
Fig. 4. Spatial expression of xEIG121L in neurula embryos. (Aâ F) Stage 15. (Gâ L) Stage 19. (A and G) Anterior views. Dorsal is upward. (B and H) Transverse sections. Dorsal is upward. (C and I) Lateral views. Anterior is to the left. (D and J) Sagittal sections. (E and K) Parasagittal sections. Anterior is to the right (D and E) or to the left (J and K). (F and L) Dorsal Views. Anterior is upward. Arrows indicate the presumptive cement gland region (A, C, D, E) or the cement gland anlage (G, I, J, K). Arrowheads indicate the presumptive hatching gland region (A, C, E, F) or the hatching gland anlage (G, I, K).
Fig. 5. Spatial expression of xEIG121L in tailbud embryos. (Aâ C) Stage 22. (Dâ F) Stage 25. (Gâ I) Stage 30. (A, F, H) Dorsal views. Anterior is upward. (B, D, G) Anterior views. Dorsal is upward. (C, E, I) Lateral views. Anterior is to the left. Arrows indicate the cement gland (B, C, D, E, G, I). Arrowheads indicate the hatching gland (B, C, D, E, G, I).
Fig. 6. Spatial expression of xEIG121L in tadpoles. (A) Lateral view. Anterior is to the left. (B) Magnified view of (A). (C) Dorsal view. Anterior is upward. (D) Magnified view of (C). (E) Horizontal section of the head. Anterior is upward. (F) Anterior view. Dorsal is upward. (G) Transverse section of the head. Dorsal is upward. Abbreviations: br, brain; ey, eye; f, fin; he, heart; le, lens; op, olfactory pit; ov, otic vesicle; pm, primary mouth; vz, ventricular zone.
