Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
J Membr Biol
2006 Jan 01;2141:1-8. doi: 10.1007/s00232-006-0064-8.
Show Gene links
Show Anatomy links
Interplay between cystic fibrosis transmembrane regulator and gap junction channels made of connexins 45, 40, 32 and 50 expressed in oocytes.
Kotsias BA
,
Salim M
,
Peracchia LL
,
Peracchia C
.
???displayArticle.abstract???
The cystic fibrosis transmembrane regulator (CFTR) is a Cl(-) channel known to influence other channels, including connexin (Cx) channels. To study the functional interaction between CFTR and gap junction channels, we coexpressed in Xenopus oocytes CFTR and either Cx45, Cx40, Cx32 or Cx50 and monitored junctional conductance (G (j)) and its sensitivity to transjunctional voltage (V (j)) by the dual voltage-clamp method. Application of forskolin induced a Cl(-) current; increased G (j) approximately 750%, 560%, 64% and 8% in Cx45, Cx40, Cx32 and Cx50, respectively; and decreased sensitivity to V (j ) gating, monitored by a change in the ratio between G (j) steady state and G (j) peak (G (j)SS/G (j)PK) at the pulse. In oocyte pairs expressing just Cx45 in one oocyte (#1) and both Cx45 and CFTR in the other (#2), with negative pulses applied to oocyte #1 forskolin application still increased G (j) and decreased the sensitivity to V (j) gating, indicating that CFTR activation is effective even when it affects only one of the two hemichannels and that the G (j) and V (j) changes are not artifacts of decreased membrane resistance in the pulsed oocyte. COOH-terminus truncation reduced the forskolin effect on Cx40 (Cx40TR) but not on Cx32 (Cx32TR) channels. The data suggest a cross-talk between CFTR and a variety of gap junction channels. Cytoskeletal scaffolding proteins and/or other intermediate cytoplasmic proteins are likely to play a role in CFTR-Cx interaction.
Bachhuber,
Cl- interference with the epithelial Na+ channel ENaC.
2005, Pubmed,
Xenbase
Bachhuber,
Cl- interference with the epithelial Na+ channel ENaC.
2005,
Pubmed
,
Xenbase
Barrio,
Gap junctions formed by connexins 26 and 32 alone and in combination are differently affected by applied voltage.
1991,
Pubmed
,
Xenbase
Bear,
Cl- channel activity in Xenopus oocytes expressing the cystic fibrosis gene.
1991,
Pubmed
,
Xenbase
Bukauskas,
Two distinct gating mechanisms in gap junction channels: CO2-sensitive and voltage-sensitive.
1997,
Pubmed
Bukauskas,
Coupling asymmetry of heterotypic connexin 45/ connexin 43-EGFP gap junctions: properties of fast and slow gating mechanisms.
2002,
Pubmed
Chanson,
Regulation of gap junctional communication by a pro-inflammatory cytokine in cystic fibrosis transmembrane conductance regulator-expressing but not cystic fibrosis airway cells.
2001,
Pubmed
Chanson,
Defective regulation of gap junctional coupling in cystic fibrosis pancreatic duct cells.
1999,
Pubmed
Cheng,
Modulation of mature cystic fibrosis transmembrane regulator protein by the PDZ domain protein CAL.
2004,
Pubmed
Cunningham,
cAMP-stimulated ion currents in Xenopus oocytes expressing CFTR cRNA.
1992,
Pubmed
,
Xenbase
Donaldson,
Update on pathogenesis of cystic fibrosis lung disease.
2003,
Pubmed
Drumm,
Chloride conductance expressed by delta F508 and other mutant CFTRs in Xenopus oocytes.
1991,
Pubmed
,
Xenbase
Gadsby,
Control of CFTR channel gating by phosphorylation and nucleotide hydrolysis.
1999,
Pubmed
Greger,
Cystic fibrosis and CFTR.
2001,
Pubmed
Harris,
Emerging issues of connexin channels: biophysics fills the gap.
2001,
Pubmed
Huang,
Defective activation of c-Src in cystic fibrosis airway epithelial cells results in loss of tumor necrosis factor-alpha-induced gap junction regulation.
2003,
Pubmed
Kausalya,
Connexin45 directly binds to ZO-1 and localizes to the tight junction region in epithelial MDCK cells.
2001,
Pubmed
Ko,
A molecular mechanism for aberrant CFTR-dependent HCO(3)(-) transport in cystic fibrosis.
2002,
Pubmed
Kotsias,
Functional interaction between CFTR and Cx45 gap junction channels expressed in oocytes.
2005,
Pubmed
,
Xenbase
Kunzelmann,
The cystic fibrosis transmembrane conductance regulator and its function in epithelial transport.
1999,
Pubmed
Kunzelmann,
CFTR: interacting with everything?
2001,
Pubmed
Lazrak,
Gap junction gating sensitivity to physiological internal calcium regardless of pH in Novikoff hepatoma cells.
1993,
Pubmed
Nagel,
CFTR fails to inhibit the epithelial sodium channel ENaC expressed in Xenopus laevis oocytes.
2005,
Pubmed
,
Xenbase
Park,
The cystic fibrosis transmembrane conductance regulator interacts with and regulates the activity of the HCO3- salvage transporter human Na+-HCO3- cotransport isoform 3.
2002,
Pubmed
Peracchia,
Effects of caffeine and ryanodine on low pHi-induced changes in gap junction conductance and calcium concentration in crayfish septate axons.
1990,
Pubmed
Peracchia,
Slow gating of gap junction channels and calmodulin.
2000,
Pubmed
,
Xenbase
Peracchia,
Is the chemical gate of connexins voltage sensitive? Behavior of Cx32 wild-type and mutant channels.
1999,
Pubmed
,
Xenbase
Peracchia,
Chemical gating of gap junction channels; roles of calcium, pH and calmodulin.
2004,
Pubmed
Peracchia,
Increase in gap junction resistance with acidification in crayfish septate axons is closely related to changes in intracellular calcium but not hydrogen ion concentration.
1990,
Pubmed
Peracchia,
Inhibition of calmodulin expression prevents low-pH-induced gap junction uncoupling in Xenopus oocytes.
1996,
Pubmed
,
Xenbase
Reczek,
Identification of EBP50: A PDZ-containing phosphoprotein that associates with members of the ezrin-radixin-moesin family.
1997,
Pubmed
Schwiebert,
CFTR is a conductance regulator as well as a chloride channel.
1999,
Pubmed
Sheppard,
Structure and function of the CFTR chloride channel.
1999,
Pubmed
Spray,
Equilibrium properties of a voltage-dependent junctional conductance.
1981,
Pubmed
,
Xenbase
Stergiopoulos,
Hetero-domain interactions as a mechanism for the regulation of connexin channels.
1999,
Pubmed
,
Xenbase
Wang,
Molecular studies of CFTR interacting proteins.
2001,
Pubmed
Wang,
Accessory protein facilitated CFTR-CFTR interaction, a molecular mechanism to potentiate the chloride channel activity.
2000,
Pubmed
