Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Mol Cell Biochem
2008 Feb 01;3091-2:33-40. doi: 10.1007/s11010-007-9640-1.
Show Gene links
Show Anatomy links
DNase I-resistant DNA-dependent protein kinase activity in Xenopus oocytes.
Kanungo J
.
???displayArticle.abstract???
DNA-dependent protein kinase (DNA-PK) is a nuclear serine/threonine protein kinase consisting of a catalytic subunit p460 (DNA-PKcs), and a DNA binding component termed Ku. DNA-PK plays a role in transcription, nonhomologous recombination, and DNA repair. Several reports have demonstrated that binding to double-stranded DNA (dsDNA) is required for the activation of DNA-PK. To date, very few reports suggest the possibility that an alternative pathway of DNA-PK activation exists without the requirement of dsDNA. In this study, direct biochemical evidence is presented to support this notion. Here, Xenopus oocytes were used as a model system because they offer the advantage of a manual enucleation process providing an extract that can be termed purely 'cytoplasmic' and the isolated nuclei (germinal vesicles) can be used to make nuclear extracts. Specific antibody-mediated pulled-down DNA-PK activity was assayed in the cytoplasmic extracts to evaluate the enzyme activity in the presence and absence of DNA. DNase I treatment did not affect the DNA-PK activity. Analyses of the association of nicked DNA with the pulled-down DNA-PK by radiolabeling the associated nicked DNA provided evidence that the cytoplasmic DNA-PK is catalytically active in absence of DNA. These results suggest that potential mechanisms occurring outside of the nucleus might activate DNA-PK, and therefore, could reveal novel functions of this enzyme.
Anderson,
DNA damage and the DNA-activated protein kinase.
1993, Pubmed
Anderson,
DNA damage and the DNA-activated protein kinase.
1993,
Pubmed
Anderson,
The nuclear serine/threonine protein kinase DNA-PK.
1992,
Pubmed
Cao,
DNA-dependent ATPase from HeLa cells is related to human Ku autoantigen.
1994,
Pubmed
Carter,
A DNA-activated protein kinase from HeLa cell nuclei.
1990,
Pubmed
de Vries,
HeLa nuclear protein recognizing DNA termini and translocating on DNA forming a regular DNA-multimeric protein complex.
1989,
Pubmed
Dumont,
Oogenesis in Xenopus laevis (Daudin). I. Stages of oocyte development in laboratory maintained animals.
1972,
Pubmed
,
Xenbase
Feldmann,
A putative homologue of the human autoantigen Ku from Saccharomyces cerevisiae.
1993,
Pubmed
Finnie,
DNA-dependent protein kinase activity is absent in xrs-6 cells: implications for site-specific recombination and DNA double-strand break repair.
1995,
Pubmed
,
Xenbase
Gottlieb,
The DNA-dependent protein kinase: requirement for DNA ends and association with Ku antigen.
1993,
Pubmed
Gurdon,
Injected nuclei in frog oocytes: fate, enlargement, and chromatin dispersal.
1976,
Pubmed
,
Xenbase
Jackson,
GC box binding induces phosphorylation of Sp1 by a DNA-dependent protein kinase.
1990,
Pubmed
Kanungo,
Prolonged incubation in seawater induces a DNA-dependent protein phosphorylation activity in Arbacia punctulata eggs.
2002,
Pubmed
Kanungo,
DNA-dependent protein phosphorylation activity in Xenopus is coupled to a Ku-like protein.
1997,
Pubmed
,
Xenbase
Laemmli,
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
1970,
Pubmed
Lees-Miller,
Human DNA-activated protein kinase phosphorylates serines 15 and 37 in the amino-terminal transactivation domain of human p53.
1992,
Pubmed
Lees-Miller,
Human cells contain a DNA-activated protein kinase that phosphorylates simian virus 40 T antigen, mouse p53, and the human Ku autoantigen.
1990,
Pubmed
Le Romancer,
The 86-kDa subunit of autoantigen Ku is a somatostatin receptor regulating protein phosphatase-2A activity.
1994,
Pubmed
Mimori,
Characterization of the DNA-binding protein antigen Ku recognized by autoantibodies from patients with rheumatic disorders.
1986,
Pubmed
Mimori,
Mechanism of interaction between Ku protein and DNA.
1986,
Pubmed
Rathmell,
Involvement of the Ku autoantigen in the cellular response to DNA double-strand breaks.
1994,
Pubmed
Roberts,
Nuclear proteins TREF1 and TREF2 bind to the transcriptional control element of the transferrin receptor gene and appear to be associated as a heterodimer.
1989,
Pubmed
Romero,
p95vav associates with the nuclear protein Ku-70.
1996,
Pubmed
Satoh,
Role of poly(ADP-ribose) formation in DNA repair.
1992,
Pubmed
Suwa,
DNA-dependent protein kinase (Ku protein-p350 complex) assembles on double-stranded DNA.
1994,
Pubmed
Taccioli,
Ku80: product of the XRCC5 gene and its role in DNA repair and V(D)J recombination.
1994,
Pubmed
Wang,
Murine monoclonal antibodies specific for conserved and non-conserved antigenic determinants of the human and murine Ku autoantigens.
1993,
Pubmed
Yaneva,
Purification of an 86-70 kDa nuclear DNA-associated protein complex.
1985,
Pubmed
Yavuzer,
DNA end-independent activation of DNA-PK mediated via association with the DNA-binding protein C1D.
1998,
Pubmed
