Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Proc Natl Acad Sci U S A
2007 Oct 02;10440:15765-70. doi: 10.1073/pnas.0704344104.
Show Gene links
Show Anatomy links
The mouse homeobox gene Noto regulates node morphogenesis, notochordal ciliogenesis, and leftright patterning.
Beckers A
,
Alten L
,
Viebahn C
,
Andre P
,
Gossler A
.
???displayArticle.abstract???
The mouse homeobox gene Noto represents the homologue of zebrafish floating head (flh) and is expressed in the organizer node and in the nascent notochord. Previous analyses suggested that Noto is required exclusively for the formation of the caudal part of the notochord. Here, we show that Noto is also essential for node morphogenesis, controlling ciliogenesis in the posterior notochord, and the establishment of laterality, whereas organizer functions in anterior-posterior patterning are apparently not compromised. In mutant embryos, left-right asymmetry of internal organs and expression of laterality markers was randomized. Mutant posterior notochord regions were variable in size and shape, cilia were shortened with highly irregular axonemal microtubuli, and basal bodies were, in part, located abnormally deep in the cytoplasm. The transcription factor Foxj1, which regulates the dynein gene Dnahc11 and is required for the correct anchoring of basal bodies in lung epithelial cells, was down-regulated in mutant nodes. Likewise, the transcription factor Rfx3, which regulates cilia growth, was not expressed in Noto mutants, and various other genes important for cilia function or assembly such as Dnahc5 and Nphp3 were down-regulated. Our results establish Noto as an essential regulator of node morphogenesis and ciliogenesis in the posterior notochord, and suggest Noto acts upstream of Foxj1 and Rfx3.
Abdelkhalek,
The mouse homeobox gene Not is required for caudal notochord development and affected by the truncate mutation.
2004, Pubmed,
Xenbase
Abdelkhalek,
The mouse homeobox gene Not is required for caudal notochord development and affected by the truncate mutation.
2004,
Pubmed
,
Xenbase
Ang,
Positive and negative signals from mesoderm regulate the expression of mouse Otx2 in ectoderm explants.
1994,
Pubmed
Bellomo,
Cell proliferation in mammalian gastrulation: the ventral node and notochord are relatively quiescent.
1996,
Pubmed
Blum,
Ciliation and gene expression distinguish between node and posterior notochord in the mammalian embryo.
2007,
Pubmed
,
Xenbase
Bonnafe,
The transcription factor RFX3 directs nodal cilium development and left-right asymmetry specification.
2004,
Pubmed
Brody,
Ciliogenesis and left-right axis defects in forkhead factor HFH-4-null mice.
2000,
Pubmed
Camus,
The organizer of the gastrulating mouse embryo.
1999,
Pubmed
Chen,
Mutation of the mouse hepatocyte nuclear factor/forkhead homologue 4 gene results in an absence of cilia and random left-right asymmetry.
1998,
Pubmed
Conlon,
A primary requirement for nodal in the formation and maintenance of the primitive streak in the mouse.
1994,
Pubmed
Cooper,
A cluster of noninvoluting endocytic cells at the margin of the zebrafish blastoderm marks the site of embryonic shield formation.
1996,
Pubmed
Danos,
Role of notochord in specification of cardiac left-right orientation in zebrafish and Xenopus.
1996,
Pubmed
,
Xenbase
De Robertis,
Spemann's organizer and self-regulation in amphibian embryos.
2006,
Pubmed
Essner,
Conserved function for embryonic nodal cilia.
2002,
Pubmed
,
Xenbase
Essner,
Kupffer's vesicle is a ciliated organ of asymmetry in the zebrafish embryo that initiates left-right development of the brain, heart and gut.
2005,
Pubmed
Feistel,
Three types of cilia including a novel 9+4 axoneme on the notochordal plate of the rabbit embryo.
2006,
Pubmed
Gluecksohn-Schoenheimer,
The Morphological Manifestations of a Dominant Mutation in Mice Affecting Tail and Urogenital System.
1943,
Pubmed
Gomperts,
Foxj1 regulates basal body anchoring to the cytoskeleton of ciliated pulmonary epithelial cells.
2004,
Pubmed
Houde,
Hippi is essential for node cilia assembly and Sonic hedgehog signaling.
2006,
Pubmed
Huangfu,
Cilia and Hedgehog responsiveness in the mouse.
2005,
Pubmed
Huangfu,
Hedgehog signalling in the mouse requires intraflagellar transport proteins.
2003,
Pubmed
Kinder,
The organizer of the mouse gastrula is composed of a dynamic population of progenitor cells for the axial mesoderm.
2001,
Pubmed
Klingensmith,
Neural induction and patterning in the mouse in the absence of the node and its derivatives.
1999,
Pubmed
Knezevic,
The organizer-associated chick homeobox gene, Gnot1, is expressed before gastrulation and regulated synergistically by activin and retinoic acid.
1995,
Pubmed
Kramer-Zucker,
Cilia-driven fluid flow in the zebrafish pronephros, brain and Kupffer's vesicle is required for normal organogenesis.
2005,
Pubmed
Lin,
Pitx2 regulates lung asymmetry, cardiac positioning and pituitary and tooth morphogenesis.
1999,
Pubmed
,
Xenbase
Männer,
Does an equivalent of the "ventral node" exist in chick embryos? A scanning electron microscopic study.
2001,
Pubmed
Marques,
The activity of the Nodal antagonist Cerl-2 in the mouse node is required for correct L/R body axis.
2004,
Pubmed
Marszalek,
Situs inversus and embryonic ciliary morphogenesis defects in mouse mutants lacking the KIF3A subunit of kinesin-II.
1999,
Pubmed
May,
Loss of the retrograde motor for IFT disrupts localization of Smo to cilia and prevents the expression of both activator and repressor functions of Gli.
2005,
Pubmed
McGrath,
Two populations of node monocilia initiate left-right asymmetry in the mouse.
2003,
Pubmed
Melby,
Specification of cell fates at the dorsal margin of the zebrafish gastrula.
1996,
Pubmed
Miething,
Ultrathin sectioning of different areas of the same semithin section.
1992,
Pubmed
Murcia,
The Oak Ridge Polycystic Kidney (orpk) disease gene is required for left-right axis determination.
2000,
Pubmed
,
Xenbase
Nonaka,
Determination of left-right patterning of the mouse embryo by artificial nodal flow.
2002,
Pubmed
Nonaka,
Randomization of left-right asymmetry due to loss of nodal cilia generating leftward flow of extraembryonic fluid in mice lacking KIF3B motor protein.
1998,
Pubmed
Okada,
Mechanism of nodal flow: a conserved symmetry breaking event in left-right axis determination.
2005,
Pubmed
Olbrich,
Mutations in a novel gene, NPHP3, cause adolescent nephronophthisis, tapeto-retinal degeneration and hepatic fibrosis.
2003,
Pubmed
Olbrich,
Mutations in DNAH5 cause primary ciliary dyskinesia and randomization of left-right asymmetry.
2002,
Pubmed
Pennekamp,
The ion channel polycystin-2 is required for left-right axis determination in mice.
2002,
Pubmed
Plouhinec,
Identification of the mammalian Not gene via a phylogenomic approach.
2004,
Pubmed
,
Xenbase
Reith,
RFX1, a transactivator of hepatitis B virus enhancer I, belongs to a novel family of homodimeric and heterodimeric DNA-binding proteins.
1994,
Pubmed
Satir,
Overview of structure and function of mammalian cilia.
2007,
Pubmed
Schweickert,
Cilia-driven leftward flow determines laterality in Xenopus.
2007,
Pubmed
,
Xenbase
Shih,
Characterizing the zebrafish organizer: microsurgical analysis at the early-shield stage.
1996,
Pubmed
Stein,
Differential activation of the clustered homeobox genes CNOT2 and CNOT1 during notogenesis in the chick.
1996,
Pubmed
,
Xenbase
Stein,
A homeobox gene involved in node, notochord and neural plate formation of chick embryos.
1995,
Pubmed
,
Xenbase
Sulik,
Morphogenesis of the murine node and notochordal plate.
1994,
Pubmed
Supp,
Mutation of an axonemal dynein affects left-right asymmetry in inversus viscerum mice.
1997,
Pubmed
Takeda,
Left-right asymmetry and kinesin superfamily protein KIF3A: new insights in determination of laterality and mesoderm induction by kif3A-/- mice analysis.
1999,
Pubmed
Tam,
Anterior patterning by synergistic activity of the early gastrula organizer and the anterior germ layer tissues of the mouse embryo.
1999,
Pubmed
Taulman,
Polaris, a protein involved in left-right axis patterning, localizes to basal bodies and cilia.
2001,
Pubmed
,
Xenbase
Viebahn,
Hensen's node.
2001,
Pubmed
Watanabe,
The left-right determinant Inversin is a component of node monocilia and other 9+0 cilia.
2003,
Pubmed
,
Xenbase
