Gen Comp Endocrinol 2008 Jun 01;1572:165-73. doi: 10.1016/j.ygcen.2008.04.012.

Expression of cyclooxygenase genes and production of prostaglandins during ovulation in the ovarian follicles of Xenopus laevis.

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Involvement of cyclooxygenase (COX) and prostaglandin (PG) synthesis during ovulation in Xenopus laevis ovarian follicles was investigated. X. laevis COX cDNAs were isolated from ovarian tissues by using reverse transcription-polymerase chain reaction (RT-PCR) followed by rapid amplification of cDNA ends (RACE). In X. laevis ovary, expression of COX-2 but not COX-1 mRNA was up-regulated during gonadotropin-induced oocyte maturation and ovulation in vitro. Elevation of PGF2* synthesis was directly correlated with the up-regulation of COX-2 mRNA. Synthesis of PGE(2) also increased during periovulatory period, however, the concentrations were much lower than those of PGF2*Progesterone (P4) also induced up-regulation of COX-2 mRNA as well as ovulation. Actinomycin D (ActD) blocked P4-induced ovulation. The inhibition of ovulation by Act D was rescued by co-treatment with exogenous PGF2* in a dose dependent manner. A non-selective COX inhibitor (indomethacin) and selective COX-2 inhibitor (NS398) strongly inhibited the hCG- and P4-dependent production of PGF2*. Inhibitory effects of selective COX-1 inhibitor (SC560) on PGF2* production were lower than that of other inhibitors. Indomethacin and NS398 blocked P4-induced ovulation. NS398 did not block hCG-induced ovulation although it strongly suppressed PGF2* production. These results suggest that (1) in Xenopus ovarian follicles, PGF2* is synthesized during periovulatory period, similar to that in mammals, (2) PGF2* synthesis is regulated by de novo transcription of COX-2 but not COX-1, (3) PGF2* is necessary for P4-induced ovulation but may not be essential for hCG-induced ovulation, and other factor(s) may be involved in the hCG-induced ovulation.

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Species referenced: Xenopus laevis
Genes referenced: ptges2