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Shugoshin regulates cohesion by driving relocalization of PP2A in Xenopus extracts.
Rivera T
,
Losada A
.
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Sister chromatid cohesion is mediated by cohesin. At the onset of mitosis, most cohesin dissociates from chromatin with the exception of a small population, present along chromosome arms and enriched at centromeres. A protein known as shugoshin (Sgo) is essential to maintain arm and centromeric cohesion until the onset of anaphase in transformed human cells, but not in other organisms like Drosophila or mouse. We have used Xenopus egg extracts to further explore this issue. Chromosomes assembled in extracts depleted of Sgo have little or no cohesin at centromeres and display centromeric cohesion defects. Unlike transformed human cells, however, arm cohesion is maintained in the absence of Sgo. Furthermore, Sgo depletion impairs the prophase dissociation of cohesin. This phenotype can be rescued by inhibition of PP2A. The protein phosphatase interacts with Sgo and accumulates at centromeres in mitosis in a Sgo-dependent manner. We propose that Sgo drives relocalization of PP2A from arms to centromeres and, in this way, coordinates release of arm cohesin with protection of centromeric cohesin in mitosis.
Boyarchuk,
Bub1 is essential for assembly of the functional inner centromere.
2007, Pubmed,
Xenbase
Boyarchuk,
Bub1 is essential for assembly of the functional inner centromere.
2007,
Pubmed
,
Xenbase
Brar,
Rec8 phosphorylation and recombination promote the step-wise loss of cohesins in meiosis.
2006,
Pubmed
Fischle,
Regulation of HP1-chromatin binding by histone H3 methylation and phosphorylation.
2005,
Pubmed
,
Xenbase
Gandhi,
Human Wapl is a cohesin-binding protein that promotes sister-chromatid resolution in mitotic prophase.
2006,
Pubmed
Goulding,
Shugoshin: a centromeric guardian senses tension.
2005,
Pubmed
Hauf,
Dissociation of cohesin from chromosome arms and loss of arm cohesion during early mitosis depends on phosphorylation of SA2.
2005,
Pubmed
Hauf,
Cohesin cleavage by separase required for anaphase and cytokinesis in human cells.
2001,
Pubmed
Hirano,
Condensins, chromosome condensation protein complexes containing XCAP-C, XCAP-E and a Xenopus homolog of the Drosophila Barren protein.
1997,
Pubmed
,
Xenbase
Hirota,
Histone H3 serine 10 phosphorylation by Aurora B causes HP1 dissociation from heterochromatin.
2005,
Pubmed
Huang,
Tripin/hSgo2 recruits MCAK to the inner centromere to correct defective kinetochore attachments.
2007,
Pubmed
Indjeian,
The centromeric protein Sgo1 is required to sense lack of tension on mitotic chromosomes.
2005,
Pubmed
Kawashima,
Shugoshin enables tension-generating attachment of kinetochores by loading Aurora to centromeres.
2007,
Pubmed
Kerrebrock,
Mei-S332, a Drosophila protein required for sister-chromatid cohesion, can localize to meiotic centromere regions.
1995,
Pubmed
Kitajima,
Human Bub1 defines the persistent cohesion site along the mitotic chromosome by affecting Shugoshin localization.
2005,
Pubmed
Kitajima,
Shugoshin collaborates with protein phosphatase 2A to protect cohesin.
2006,
Pubmed
Kitajima,
The conserved kinetochore protein shugoshin protects centromeric cohesion during meiosis.
2004,
Pubmed
Kudo,
Resolution of chiasmata in oocytes requires separase-mediated proteolysis.
2006,
Pubmed
,
Xenbase
Kueng,
Wapl controls the dynamic association of cohesin with chromatin.
2006,
Pubmed
LeBlanc,
The mitotic centromeric protein MEI-S332 and its role in sister-chromatid cohesion.
1999,
Pubmed
Lee,
Unified mode of centromeric protection by shugoshin in mammalian oocytes and somatic cells.
2008,
Pubmed
Lengronne,
Cohesin relocation from sites of chromosomal loading to places of convergent transcription.
2004,
Pubmed
Lipp,
Aurora B controls the association of condensin I but not condensin II with mitotic chromosomes.
2007,
Pubmed
Losada,
Identification and characterization of SA/Scc3p subunits in the Xenopus and human cohesin complexes.
2000,
Pubmed
,
Xenbase
Losada,
Functional contribution of Pds5 to cohesin-mediated cohesion in human cells and Xenopus egg extracts.
2005,
Pubmed
,
Xenbase
Losada,
Cohesin release is required for sister chromatid resolution, but not for condensin-mediated compaction, at the onset of mitosis.
2002,
Pubmed
,
Xenbase
Losada,
Identification of Xenopus SMC protein complexes required for sister chromatid cohesion.
1998,
Pubmed
,
Xenbase
Losada,
Dynamic molecular linkers of the genome: the first decade of SMC proteins.
2005,
Pubmed
MacCallum,
ISWI remodeling complexes in Xenopus egg extracts: identification as major chromosomal components that are regulated by INCENP-aurora B.
2002,
Pubmed
,
Xenbase
Marston,
Meiosis: cell-cycle controls shuffle and deal.
2004,
Pubmed
McGuinness,
Shugoshin prevents dissociation of cohesin from centromeres during mitosis in vertebrate cells.
2005,
Pubmed
Nakajima,
The complete removal of cohesin from chromosome arms depends on separase.
2007,
Pubmed
Nasmyth,
The structure and function of SMC and kleisin complexes.
2005,
Pubmed
Ono,
Spatial and temporal regulation of Condensins I and II in mitotic chromosome assembly in human cells.
2004,
Pubmed
Perera,
Bub1 maintains centromeric cohesion by activation of the spindle checkpoint.
2007,
Pubmed
Rabitsch,
Two fission yeast homologs of Drosophila Mei-S332 are required for chromosome segregation during meiosis I and II.
2004,
Pubmed
Resnick,
INCENP and Aurora B promote meiotic sister chromatid cohesion through localization of the Shugoshin MEI-S332 in Drosophila.
2006,
Pubmed
Riedel,
Protein phosphatase 2A protects centromeric sister chromatid cohesion during meiosis I.
2006,
Pubmed
Salic,
Vertebrate shugoshin links sister centromere cohesion and kinetochore microtubule stability in mitosis.
2004,
Pubmed
,
Xenbase
Sampath,
The chromosomal passenger complex is required for chromatin-induced microtubule stabilization and spindle assembly.
2004,
Pubmed
,
Xenbase
Sumara,
The dissociation of cohesin from chromosomes in prophase is regulated by Polo-like kinase.
2002,
Pubmed
,
Xenbase
Tang,
Human Bub1 protects centromeric sister-chromatid cohesion through Shugoshin during mitosis.
2004,
Pubmed
Tang,
PP2A is required for centromeric localization of Sgo1 and proper chromosome segregation.
2006,
Pubmed
Terret,
The meiosis I-to-meiosis II transition in mouse oocytes requires separase activity.
2003,
Pubmed
Vagnarelli,
Analysis of Scc1-deficient cells defines a key metaphase role of vertebrate cohesin in linking sister kinetochores.
2004,
Pubmed
Vanoosthuyse,
Shugoshin 2 regulates localization of the chromosomal passenger proteins in fission yeast mitosis.
2007,
Pubmed
Vass,
Depletion of Drad21/Scc1 in Drosophila cells leads to instability of the cohesin complex and disruption of mitotic progression.
2003,
Pubmed
Vaur,
Control of Shugoshin function during fission-yeast meiosis.
2005,
Pubmed
Vigneron,
Kinetochore localization of spindle checkpoint proteins: who controls whom?
2004,
Pubmed
,
Xenbase
Waizenegger,
Two distinct pathways remove mammalian cohesin from chromosome arms in prophase and from centromeres in anaphase.
2000,
Pubmed
,
Xenbase
Yu,
The Aurora kinase Ipl1 maintains the centromeric localization of PP2A to protect cohesin during meiosis.
2007,
Pubmed
