Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Polycomb proteins remain bound to chromatin and DNA during DNA replication in vitro.
Francis NJ
,
Follmer NE
,
Simon MD
,
Aghia G
,
Butler JD
.
???displayArticle.abstract???
The transcriptional status of a gene can be maintained through multiple rounds of cell division during development. This epigenetic effect is believed to reflect heritable changes in chromatin folding and histone modifications or variants at target genes, but little is known about how these chromatin features are inherited through cell division. A particular challenge for maintaining transcription states is DNA replication, which disrupts or dilutes chromatin-associated proteins and histone modifications. PRC1-class Polycomb group protein complexes are essential for development and are thought to heritably silence transcription by altering chromatin folding and histone modifications. It is not known whether these complexes and their effects are maintained during DNA replication or subsequently re-established. We find that when PRC1-class Polycomb complex-bound chromatin or DNA is replicated in vitro, Polycomb complexes remain bound to replicated templates. Retention of Polycomb proteins through DNA replication may contribute to maintenance of transcriptional silencing through cell division.
Aggarwal,
Chromatin regulates origin activity in Drosophila follicle cells.
2004,
Pubmed
Annunziato,
Split decision: what happens to nucleosomes during DNA replication?
2005,
Pubmed
Bantignies,
Inheritance of Polycomb-dependent chromosomal interactions in Drosophila.
2003,
Pubmed
Benson,
Modifications of H3 and H4 during chromatin replication, nucleosome assembly, and histone exchange.
2006,
Pubmed
Beuchle,
Polycomb group proteins and heritable silencing of Drosophila Hox genes.
2001,
Pubmed
Bonne-Andrea,
In vitro replication through nucleosomes without histone displacement.
1990,
Pubmed
Breiling,
General transcription factors bind promoters repressed by Polycomb group proteins.
2001,
Pubmed
Brown,
The Drosophila Polycomb group gene pleiohomeotic encodes a DNA binding protein with homology to the transcription factor YY1.
1998,
Pubmed
Cao,
The functions of E(Z)/EZH2-mediated methylation of lysine 27 in histone H3.
2004,
Pubmed
Cao,
Role of Bmi-1 and Ring1A in H2A ubiquitylation and Hox gene silencing.
2005,
Pubmed
Cao,
Role of histone H3 lysine 27 methylation in Polycomb-group silencing.
2002,
Pubmed
Clark,
A nucleosome core is transferred out of the path of a transcribing polymerase.
1992,
Pubmed
de Napoles,
Polycomb group proteins Ring1A/B link ubiquitylation of histone H2A to heritable gene silencing and X inactivation.
2004,
Pubmed
Dodd,
Theoretical analysis of epigenetic cell memory by nucleosome modification.
2007,
Pubmed
Elderkin,
A phosphorylated form of Mel-18 targets the Ring1B histone H2A ubiquitin ligase to chromatin.
2007,
Pubmed
Fischle,
Molecular basis for the discrimination of repressive methyl-lysine marks in histone H3 by Polycomb and HP1 chromodomains.
2003,
Pubmed
Francis,
Chromatin compaction by a polycomb group protein complex.
2004,
Pubmed
Francis,
Reconstitution of a functional core polycomb repressive complex.
2001,
Pubmed
Fritsch,
The DNA-binding polycomb group protein pleiohomeotic mediates silencing of a Drosophila homeotic gene.
1999,
Pubmed
Grimaud,
From genetics to epigenetics: the tale of Polycomb group and trithorax group genes.
2006,
Pubmed
Groth,
Chromatin challenges during DNA replication and repair.
2007,
Pubmed
Hansen,
A model for transmission of the H3K27me3 epigenetic mark.
2008,
Pubmed
Jackman,
Methods for synchronizing cells at specific stages of the cell cycle.
2001,
Pubmed
King,
Native and recombinant polycomb group complexes establish a selective block to template accessibility to repress transcription in vitro.
2002,
Pubmed
King,
Analysis of a polycomb group protein defines regions that link repressive activity on nucleosomal templates to in vivo function.
2005,
Pubmed
Klymenko,
A Polycomb group protein complex with sequence-specific DNA-binding and selective methyl-lysine-binding activities.
2006,
Pubmed
Kohn,
Chromatin challenges during DNA replication: a systems representation.
2008,
Pubmed
Kwong,
Stability and dynamics of polycomb target sites in Drosophila development.
2008,
Pubmed
Lagarou,
dKDM2 couples histone H2A ubiquitylation to histone H3 demethylation during Polycomb group silencing.
2008,
Pubmed
Lanzuolo,
Polycomb response elements mediate the formation of chromosome higher-order structures in the bithorax complex.
2007,
Pubmed
Lavigne,
Propagation of silencing; recruitment and repression of naive chromatin in trans by polycomb repressed chromatin.
2004,
Pubmed
Levine,
The core of the polycomb repressive complex is compositionally and functionally conserved in flies and humans.
2002,
Pubmed
Li,
Simian virus 40 DNA replication in vitro.
1984,
Pubmed
Logie,
Catalytic activity of the yeast SWI/SNF complex on reconstituted nucleosome arrays.
1997,
Pubmed
Marchetti,
Differential expression of the Drosophila BX-C in polytene chromosomes in cells of larval fat bodies: a cytological approach to identifying in vivo targets of the homeotic Ubx, Abd-A and Abd-B proteins.
2003,
Pubmed
Mihaly,
A conserved sequence motif in Polycomb-response elements.
1998,
Pubmed
Min,
Structural basis for specific binding of Polycomb chromodomain to histone H3 methylated at Lys 27.
2003,
Pubmed
Mishra,
The iab-7 polycomb response element maps to a nucleosome-free region of chromatin and requires both GAGA and pleiohomeotic for silencing activity.
2001,
Pubmed
Mito,
Histone replacement marks the boundaries of cis-regulatory domains.
2007,
Pubmed
Mohd-Sarip,
Architecture of a polycomb nucleoprotein complex.
2006,
Pubmed
Mohd-Sarip,
Synergistic recognition of an epigenetic DNA element by Pleiohomeotic and a Polycomb core complex.
2005,
Pubmed
Moshkin,
The bithorax complex of Drosophila melanogaster: Underreplication and morphology in polytene chromosomes.
2001,
Pubmed
Ng,
Epigenetic inheritance of cell differentiation status.
2008,
Pubmed
Nightingale,
Histone modifications: signalling receptors and potential elements of a heritable epigenetic code.
2006,
Pubmed
Papp,
Histone trimethylation and the maintenance of transcriptional ON and OFF states by trxG and PcG proteins.
2006,
Pubmed
Pietersen,
Stem cell regulation by polycomb repressors: postponing commitment.
2008,
Pubmed
Polach,
Mechanism of protein access to specific DNA sequences in chromatin: a dynamic equilibrium model for gene regulation.
1995,
Pubmed
Poux,
Recruitment of components of Polycomb Group chromatin complexes in Drosophila.
2001,
Pubmed
Puschendorf,
PRC1 and Suv39h specify parental asymmetry at constitutive heterochromatin in early mouse embryos.
2008,
Pubmed
Ringrose,
Epigenetic regulation of cellular memory by the Polycomb and Trithorax group proteins.
2004,
Pubmed
Ringrose,
Distinct contributions of histone H3 lysine 9 and 27 methylation to locus-specific stability of polycomb complexes.
2004,
Pubmed
Ringrose,
Polycomb/Trithorax response elements and epigenetic memory of cell identity.
2007,
Pubmed
Ringrose,
Polycomb comes of age: genome-wide profiling of target sites.
2007,
Pubmed
Sanchez,
The efficiency and timing of plasmid DNA replication in Xenopus eggs: correlations to the extent of prior chromatin assembly.
1992,
Pubmed
,
Xenbase
Saurin,
A Drosophila Polycomb group complex includes Zeste and dTAFII proteins.
2001,
Pubmed
Schoeftner,
Recruitment of PRC1 function at the initiation of X inactivation independent of PRC2 and silencing.
2006,
Pubmed
Schwartz,
Genome-wide analysis of Polycomb targets in Drosophila melanogaster.
2006,
Pubmed
Schwartz,
Polycomb complexes and epigenetic states.
2008,
Pubmed
Shao,
Stabilization of chromatin structure by PRC1, a Polycomb complex.
1999,
Pubmed
Simon,
The site-specific installation of methyl-lysine analogs into recombinant histones.
2007,
Pubmed
,
Xenbase
Simon,
Programming off and on states in chromatin: mechanisms of Polycomb and trithorax group complexes.
2002,
Pubmed
Stillman,
Replication and supercoiling of simian virus 40 DNA in cell extracts from human cells.
1985,
Pubmed
Studitsky,
A histone octamer can step around a transcribing polymerase without leaving the template.
1994,
Pubmed
Terranova,
Polycomb group proteins Ezh2 and Rnf2 direct genomic contraction and imprinted repression in early mouse embryos.
2008,
Pubmed
Wang,
Role of histone H2A ubiquitination in Polycomb silencing.
2004,
Pubmed
Wang,
Hierarchical recruitment of polycomb group silencing complexes.
2004,
Pubmed
