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A transition metal complex targeted for the inhibition of a subset of zinc finger transcription factors has been synthesized and tested in Xenopus laevis. A Co(III) Schiff base complex modified with a 17-bp DNA sequence is designed to selectively inhibit Snail family transcription factors. The oligonucleotide-conjugated Co(III) complex prevents Slug, Snail, and Sip1 from binding their DNA targets whereas other transcription factors are still able to interact with their target DNA. The attachment of the oligonucleotide to the Co(III) complex increases specificity 150-fold over the unconjugated complex. Studies demonstrate that neither the oligo, or the Co(III) Schiff base complex alone, are sufficient for inactivation of Slug at concentrations that the conjugated complex mediates inhibition. Slug, Snail, and Sip1 have been implicated in the regulation of epithelial-to-mesenchymal transition in development and cancer. A complex targeted to inactivate their transcriptional activity could prove valuable as an experimental tool and a cancer therapeutic.
Aybar,
Snail precedes slug in the genetic cascade required for the specification and migration of the Xenopus neural crest.
2003, Pubmed,
Xenbase
Aybar,
Snail precedes slug in the genetic cascade required for the specification and migration of the Xenopus neural crest.
2003,
Pubmed
,
Xenbase
Balaeff,
Structure prediction of a complex between the chromosomal protein HMG-D and DNA.
1998,
Pubmed
Bellmeyer,
The protooncogene c-myc is an essential regulator of neural crest formation in xenopus.
2003,
Pubmed
,
Xenbase
Blanco,
Correlation of Snail expression with histological grade and lymph node status in breast carcinomas.
2002,
Pubmed
Blum,
Isolation of a myoglobin molten globule by selective cobalt(III)-induced unfolding.
1998,
Pubmed
Bolós,
The transcription factor Slug represses E-cadherin expression and induces epithelial to mesenchymal transitions: a comparison with Snail and E47 repressors.
2003,
Pubmed
Carl,
Inhibition of neural crest migration in Xenopus using antisense slug RNA.
1999,
Pubmed
,
Xenbase
Cheung,
The transcriptional control of trunk neural crest induction, survival, and delamination.
2005,
Pubmed
Eisaki,
XSIP1, a member of two-handed zinc finger proteins, induced anterior neural markers in Xenopus laevis animal cap.
2000,
Pubmed
,
Xenbase
Epstein,
Efficacy of topical cobalt chelate CTC-96 against adenovirus in a cell culture model and against adenovirus keratoconjunctivitis in a rabbit model.
2006,
Pubmed
Goding,
Mitf from neural crest to melanoma: signal transduction and transcription in the melanocyte lineage.
2000,
Pubmed
Gray,
Circular dichroism spectroscopy of DNA.
1992,
Pubmed
Hartwig,
Zinc finger proteins as potential targets for toxic metal ions: differential effects on structure and function.
2001,
Pubmed
Kuperwasser,
A mouse model of human breast cancer metastasis to human bone.
2005,
Pubmed
Kuphal,
Snail-regulated genes in malignant melanoma.
2005,
Pubmed
LaBonne,
Snail-related transcriptional repressors are required in Xenopus for both the induction of the neural crest and its subsequent migration.
2000,
Pubmed
,
Xenbase
Larabee,
Mechanisms of aurothiomalate-Cys2His2 zinc finger interactions.
2005,
Pubmed
,
Xenbase
Louie,
A cobalt complex that selectively disrupts the structure and function of zinc fingers.
1998,
Pubmed
Martínez-Estrada,
The transcription factors Slug and Snail act as repressors of Claudin-1 expression in epithelial cells.
2006,
Pubmed
Mayor,
Induction of the prospective neural crest of Xenopus.
1995,
Pubmed
,
Xenbase
Papworth,
Inhibition of herpes simplex virus 1 gene expression by designer zinc-finger transcription factors.
2003,
Pubmed
Peinado,
Transcriptional regulation of cadherins during development and carcinogenesis.
2004,
Pubmed
Peinado,
Snail mediates E-cadherin repression by the recruitment of the Sin3A/histone deacetylase 1 (HDAC1)/HDAC2 complex.
2004,
Pubmed
Sakai,
Cooperative action of Sox9, Snail2 and PKA signaling in early neural crest development.
2006,
Pubmed
Savagner,
The zinc-finger protein slug causes desmosome dissociation, an initial and necessary step for growth factor-induced epithelial-mesenchymal transition.
1997,
Pubmed
Schwartz,
Herpes simplex virus type 1 entry is inhibited by the cobalt chelate complex CTC-96.
2001,
Pubmed
Shiozaki,
E-cadherin mediated adhesion system in cancer cells.
1996,
Pubmed
Takeuchi,
Inhibition of thermolysin and human alpha-thrombin by cobalt(III) Schiff base complexes.
1999,
Pubmed
Toledano,
N-terminal DNA-binding domains contribute to differential DNA-binding specificities of NF-kappa B p50 and p65.
1993,
Pubmed
Vallin,
Cloning and characterization of three Xenopus slug promoters reveal direct regulation by Lef/beta-catenin signaling.
2001,
Pubmed
,
Xenbase
Vandewalle,
SIP1/ZEB2 induces EMT by repressing genes of different epithelial cell-cell junctions.
2005,
Pubmed
Vernon,
Slug stability is dynamically regulated during neural crest development by the F-box protein Ppa.
2006,
Pubmed
,
Xenbase
Watkin,
Mechanisms regulating the cadmium-mediated suppression of Sp1 transcription factor activity in alveolar epithelial cells.
2003,
Pubmed
Weinstein,
Mechanisms of disease: Oncogene addiction--a rationale for molecular targeting in cancer therapy.
2006,
Pubmed
Yang,
Pak1 phosphorylation of snail, a master regulator of epithelial-to-mesenchyme transition, modulates snail's subcellular localization and functions.
2005,
Pubmed
Zabel,
DNA binding of purified transcription factor NF-kappa B. Affinity, specificity, Zn2+ dependence, and differential half-site recognition.
1991,
Pubmed
