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Ren J
,
Liu Z
,
Gao X
,
Jin C
,
Ye M
,
Zou H
,
Wen L
,
Zhang Z
,
Xue Y
,
Yao X
.
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During cell division/mitosis, a specific subset of proteins is spatially and temporally assembled into protein super complexes in three distinct regions, i.e. centrosome/spindle pole, kinetochore/centromere and midbody/cleavage furrow/phragmoplast/bud neck, and modulates cell division process faithfully. Although many experimental efforts have been carried out to investigate the characteristics of these proteins, no integrated database was available. Here, we present the MiCroKit database (http://microkit.biocuckoo.org) of proteins that localize in midbody, centrosome and/or kinetochore. We collected into the MiCroKit database experimentally verified microkit proteins from the scientific literature that have unambiguous supportive evidence for subcellular localization under fluorescent microscope. The current version of MiCroKit 3.0 provides detailed information for 1489 microkit proteins from seven model organisms, including Saccharomyces cerevisiae, Schizasaccharomyces pombe, Caenorhabditis elegans, Drosophila melanogaster, Xenopus laevis, Mus musculus and Homo sapiens. Moreover, the orthologous information was provided for these microkit proteins, and could be a useful resource for further experimental identification. The online service of MiCroKit database was implemented in PHP + MySQL + JavaScript, while the local packages were developed in JAVA 1.5 (J2SE 5.0).
Figure 1. The search option of MiCroKit 3.0 database. (A) Users could simply input âsurvivinâ for querying. (B) The results will be shown in a tabular format. Users could click on the MiCroKit ID (MCK-HS-00222) to visualize the detailed information. (C) The detailed information of human Survivin. The orthologous information was pre-calculated and manually checked.
Figure 2. Five advance options in MiCroKit 3.0. (A) Advance search allows users to input up to three terms for querying; (B) browse and BLAST search; (C) orthologous group browse and Pairwise orthologous browse; (D) the default example of pairwise orthologous browse. The orthologous information (identity ⥠20%) between M. musculus and H. sapiens will be shown in details. The proteins collected in the MiCroKit database are marked in bold.
Figure 3. The statistics of localization distributions of microkit proteins from seven organisms, separately.
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