Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Am J Physiol Renal Physiol
2010 May 01;2985:F1113-7. doi: 10.1152/ajprenal.00592.2009.
Show Gene links
Show Anatomy links
Regulation of renal tubular glucose reabsorption by Akt2/PKBβ.
Kempe DS
,
Siraskar G
,
Fröhlich H
,
Umbach AT
,
Stübs M
,
Weiss F
,
Ackermann TF
,
Völkl H
,
Birnbaum MJ
,
Pearce D
,
Föller M
,
Lang F
.
???displayArticle.abstract???
Akt/PKB is known to regulate the facilitative glucose carrier GLUT4. Nothing is known, however, of the role of Akt/PKB in the regulation of renal epithelial transport. To explore whether Akt2/PKBβ influences the Na(+)-coupled glucose cotransporter SGLT1, human SGLT1 was expressed in Xenopus laevis oocytes with or without Akt/PKB, and electrogenic glucose transport was determined by dual-electrode voltage clamp. The coexpression of Akt/PKB in SGLT1-expressing oocytes was followed by an increase in glucose-induced currents. To study the functional significance of Akt/PKB-sensitive renal glucose transport, further experiments were performed in gene-targeted mice lacking functional Akt2/PKBβ (akt2(-/-)) and in their wild-type littermates (akt2(+/+)). Plasma glucose concentration was significantly higher in akt2(-/-) mice than in akt2(+/+) mice but was virtually identical to the plasma glucose concentration in fructose-treated akt2(+/+) mice. Urinary glucose excretion was significantly higher in akt2(-/-) mice compared with akt2(+/+) mice with or without fructose treatment. Moreover, the glucose-induced depolarization of proximal tubular cells was significantly smaller in isolated, perfused renal tubules from akt2(-/-) mice than in those from akt2(+/+) mice. In conclusion, Akt2/PKBβ plays a role in the regulation of renal glucose transport.
Ackermann,
SGK1-sensitive renal tubular glucose reabsorption in diabetes.
2009, Pubmed
Ackermann,
SGK1-sensitive renal tubular glucose reabsorption in diabetes.
2009,
Pubmed
Alessi,
Mechanism of activation of protein kinase B by insulin and IGF-1.
1996,
Pubmed
Alessi,
3-Phosphoinositide-dependent protein kinase-1 (PDK1): structural and functional homology with the Drosophila DSTPK61 kinase.
1997,
Pubmed
Artunc,
Impaired intestinal and renal glucose transport in PDK-1 hypomorphic mice.
2006,
Pubmed
Balazs,
DHEA induces 11 -HSD2 by acting on CCAAT/enhancer-binding proteins.
2008,
Pubmed
Beck,
Nitric oxide upregulates induction of PDGF receptor-alpha expression in rat renal mesangial cells and in anti-Thy-1 glomerulonephritis.
2005,
Pubmed
Boehmer,
Modulation of the voltage-gated potassium channel Kv1.5 by the SGK1 protein kinase involves inhibition of channel ubiquitination.
2008,
Pubmed
,
Xenbase
Boehmer,
The peptide transporter PEPT2 is targeted by the protein kinase SGK1 and the scaffold protein NHERF2.
2008,
Pubmed
,
Xenbase
Carranza,
Signaling cascade of insulin-induced stimulation of L-dopa uptake in renal proximal tubule cells.
2008,
Pubmed
Cho,
Insulin resistance and a diabetes mellitus-like syndrome in mice lacking the protein kinase Akt2 (PKB beta).
2001,
Pubmed
Cho,
Akt1/PKBalpha is required for normal growth but dispensable for maintenance of glucose homeostasis in mice.
2001,
Pubmed
Dieter,
Regulation of glucose transporter SGLT1 by ubiquitin ligase Nedd4-2 and kinases SGK1, SGK3, and PKB.
2004,
Pubmed
,
Xenbase
Green,
Use of Akt inhibitor and a drug-resistant mutant validates a critical role for protein kinase B/Akt in the insulin-dependent regulation of glucose and system A amino acid uptake.
2008,
Pubmed
Huang,
Blunted hypertensive effect of combined fructose and high-salt diet in gene-targeted mice lacking functional serum- and glucocorticoid-inducible kinase SGK1.
2006,
Pubmed
Ishiki,
Minireview: recent developments in the regulation of glucose transporter-4 traffic: new signals, locations, and partners.
2005,
Pubmed
Kobayashi,
Activation of serum- and glucocorticoid-regulated protein kinase by agonists that activate phosphatidylinositide 3-kinase is mediated by 3-phosphoinositide-dependent protein kinase-1 (PDK1) and PDK2.
1999,
Pubmed
Koulen,
Progesterone potentiates IP(3)-mediated calcium signaling through Akt/PKB.
2008,
Pubmed
Lang,
Deranged transcriptional regulation of cell-volume-sensitive kinase hSGK in diabetic nephropathy.
2000,
Pubmed
,
Xenbase
Lang,
(Patho)physiological significance of the serum- and glucocorticoid-inducible kinase isoforms.
2006,
Pubmed
Lawlor,
Essential role of PDK1 in regulating cell size and development in mice.
2002,
Pubmed
Lee,
Akt mediates the effect of insulin on epithelial sodium channels by inhibiting Nedd4-2.
2007,
Pubmed
Lennon,
Hemopexin induces nephrin-dependent reorganization of the actin cytoskeleton in podocytes.
2008,
Pubmed
Lim,
Bradykinin stimulates glutamate uptake via both B1R and B2R activation in a human retinal pigment epithelial cells.
2008,
Pubmed
Mannack,
Taurolithocholic acid-3 sulfate impairs insulin signaling in cultured rat hepatocytes and perfused rat liver.
2008,
Pubmed
Menne,
The continuous erythropoietin receptor activator affects different pathways of diabetic renal injury.
2007,
Pubmed
Qian,
Sirolimus reduces polycystic liver volume in ADPKD patients.
2008,
Pubmed
Strutz-Seebohm,
Comparison of potent Kv1.5 potassium channel inhibitors reveals the molecular basis for blocking kinetics and binding mode.
2007,
Pubmed
Vallon,
Role of KCNE1-dependent K+ fluxes in mouse proximal tubule.
2001,
Pubmed
Vallon,
In vivo studies of the genetically modified mouse kidney.
2003,
Pubmed
Vallon,
KCNQ1-dependent transport in renal and gastrointestinal epithelia.
2005,
Pubmed
Vaughan-Jones,
Pushing and pulling the cardiac sodium/hydrogen exchanger.
2008,
Pubmed
Wagner,
The use of Xenopus laevis oocytes for the functional characterization of heterologously expressed membrane proteins.
2000,
Pubmed
,
Xenbase
Wagner,
Mitogenic signaling via platelet-derived growth factor beta in metanephric mesenchymal cells.
2007,
Pubmed
Whiteman,
Role of Akt/protein kinase B in metabolism.
2002,
Pubmed
Williams,
Activation of the G(i) heterotrimeric G protein by ANCA IgG F(ab')2 fragments is necessary but not sufficient to stimulate the recruitment of those downstream mediators used by intact ANCA IgG.
2003,
Pubmed
Wright,
Active sugar transport in health and disease.
2007,
Pubmed
Wu,
PKC-beta1 mediates glucose-induced Akt activation and TGF-beta1 upregulation in mesangial cells.
2009,
Pubmed
Zeng,
Biliverdin reductase mediates hypoxia-induced EMT via PI3-kinase and Akt.
2008,
Pubmed
