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Org Lett
2010 Sep 03;1217:3776-9. doi: 10.1021/ol101408f.
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Preparation of translationally competent tRNA by direct chemical acylation.
Duffy NH
,
Dougherty DA
.
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Nonsense codon suppression for unnatural amino acid incorporation requires the preparation of a suppressor aminoacyl-tRNA. Chemical acylation strategies are general but inefficient and arduous. A recent report (J. Am. Chem. Soc. 2007, 129, 15848) showed acylation of RNA mediated by lanthanum(III) using amino acid phosphate esters. The successful implementation of this methodology to full-length suppressor tRNA is described, and it is shown that the derived aminoacyl-tRNA is translationally competent in Xenopus oocytes.
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