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J Biol Chem
2010 Oct 15;28542:32576-85. doi: 10.1074/jbc.M110.153817.
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Rod/Zw10 complex is required for PIASy-dependent centromeric SUMOylation.
Ryu H
,
Azuma Y
.
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SUMO conjugation of cellular proteins is essential for proper progression of mitosis. PIASy, a SUMO E3 ligase, is required for mitotic SUMOylation of chromosomal proteins, yet the regulatory mechanism behind the PIASy-dependent SUMOylation during mitosis has not been determined. Using a series of truncated PIASy proteins, we have found that the N terminus of PIASy is not required for SUMO modification in vitro but is essential for mitotic SUMOylation in Xenopus egg extracts. We demonstrate that swapping the N terminus of PIASy protein with the corresponding region of other PIAS family members abolishes chromosomal binding and mitotic SUMOylation. We further show that the N-terminal domain of PIASy is sufficient for centromeric localization. We identified that the N-terminal domain of PIASy interacts with the Rod/Zw10 complex, and immunofluorescence further reveals that PIASy colocalizes with Rod/Zw10 in the centromeric region. We show that the Rod/Zw10 complex interacts with the first 47 residues of PIASy which were particularly important for mitotic SUMOylation. Finally, we show that depletion of Rod compromises the centromeric localization of PIASy and SUMO2/3 in mitosis. Together, we demonstrate a fundamental mechanism of PIASy to localize in the centromeric region of chromosome to execute centromeric SUMOylation during mitosis.
Aravind,
SAP - a putative DNA-binding motif involved in chromosomal organization.
2000, Pubmed
Aravind,
SAP - a putative DNA-binding motif involved in chromosomal organization.
2000,
Pubmed
Azuma,
SUMO-2/3 regulates topoisomerase II in mitosis.
2003,
Pubmed
,
Xenbase
Azuma,
Expression and regulation of the mammalian SUMO-1 E1 enzyme.
2001,
Pubmed
Azuma,
PIASy mediates SUMO-2 conjugation of Topoisomerase-II on mitotic chromosomes.
2005,
Pubmed
,
Xenbase
Azuma,
Analysis of SUMOylation of topoisomerase IIalpha with Xenopus egg extracts.
2009,
Pubmed
,
Xenbase
Basto,
Rough deal and Zw10 are required for the metaphase checkpoint in Drosophila.
2000,
Pubmed
Basto,
In vivo dynamics of the rough deal checkpoint protein during Drosophila mitosis.
2004,
Pubmed
Bernier-Villamor,
Structural basis for E2-mediated SUMO conjugation revealed by a complex between ubiquitin-conjugating enzyme Ubc9 and RanGAP1.
2002,
Pubmed
Biggins,
Genes involved in sister chromatid separation and segregation in the budding yeast Saccharomyces cerevisiae.
2001,
Pubmed
Bossis,
SUMO: regulating the regulator.
2006,
Pubmed
Buffin,
Recruitment of Mad2 to the kinetochore requires the Rod/Zw10 complex.
2005,
Pubmed
Dasso,
Emerging roles of the SUMO pathway in mitosis.
2008,
Pubmed
Dawlaty,
Resolution of sister centromeres requires RanBP2-mediated SUMOylation of topoisomerase IIalpha.
2008,
Pubmed
Duval,
The 'PINIT' motif, of a newly identified conserved domain of the PIAS protein family, is essential for nuclear retention of PIAS3L.
2003,
Pubmed
Hay,
SUMO: a history of modification.
2005,
Pubmed
Hecker,
Specification of SUMO1- and SUMO2-interacting motifs.
2006,
Pubmed
Johnson,
Protein modification by SUMO.
2004,
Pubmed
Johnson,
An E3-like factor that promotes SUMO conjugation to the yeast septins.
2001,
Pubmed
Karess,
Rod-Zw10-Zwilch: a key player in the spindle checkpoint.
2005,
Pubmed
Kops,
ZW10 links mitotic checkpoint signaling to the structural kinetochore.
2005,
Pubmed
,
Xenbase
Kotaja,
PIAS proteins modulate transcription factors by functioning as SUMO-1 ligases.
2002,
Pubmed
Lee,
PIAS1 confers DNA-binding specificity on the Msx1 homeoprotein.
2006,
Pubmed
Lu,
The RZZ complex and the spindle assembly checkpoint.
2009,
Pubmed
Okubo,
NMR structure of the N-terminal domain of SUMO ligase PIAS1 and its interaction with tumor suppressor p53 and A/T-rich DNA oligomers.
2004,
Pubmed
Palvimo,
PIAS proteins as regulators of small ubiquitin-related modifier (SUMO) modifications and transcription.
2007,
Pubmed
Pichler,
The nucleoporin RanBP2 has SUMO1 E3 ligase activity.
2002,
Pubmed
Pichler,
The RanBP2 SUMO E3 ligase is neither HECT- nor RING-type.
2004,
Pubmed
Reindle,
Multiple domains in Siz SUMO ligases contribute to substrate selectivity.
2006,
Pubmed
Rytinki,
PIAS proteins: pleiotropic interactors associated with SUMO.
2009,
Pubmed
Ryu,
PIASy mediates SUMO-2/3 conjugation of poly(ADP-ribose) polymerase 1 (PARP1) on mitotic chromosomes.
2010,
Pubmed
,
Xenbase
Santaguida,
The life and miracles of kinetochores.
2009,
Pubmed
Scaërou,
The ZW10 and Rough Deal checkpoint proteins function together in a large, evolutionarily conserved complex targeted to the kinetochore.
2001,
Pubmed
Scaërou,
The rough deal protein is a new kinetochore component required for accurate chromosome segregation in Drosophila.
1999,
Pubmed
Sekiyama,
Structure of the small ubiquitin-like modifier (SUMO)-interacting motif of MBD1-containing chromatin-associated factor 1 bound to SUMO-3.
2008,
Pubmed
Shayeghi,
Characterisation of Schizosaccharomyces pombe rad31, a UBA-related gene required for DNA damage tolerance.
1997,
Pubmed
Starr,
ZW10 helps recruit dynactin and dynein to the kinetochore.
1998,
Pubmed
Suzuki,
Solution structures and DNA binding properties of the N-terminal SAP domains of SUMO E3 ligases from Saccharomyces cerevisiae and Oryza sativa.
2009,
Pubmed
Takahashi,
Comparative analysis of yeast PIAS-type SUMO ligases in vivo and in vitro.
2003,
Pubmed
Tanaka,
Characterization of a fission yeast SUMO-1 homologue, pmt3p, required for multiple nuclear events, including the control of telomere length and chromosome segregation.
1999,
Pubmed
Tolkunova,
PIAS proteins as repressors of Oct4 function.
2007,
Pubmed
Torras-Llort,
Focus on the centre: the role of chromatin on the regulation of centromere identity and function.
2009,
Pubmed
Wang,
Three classes of genes mutated in colorectal cancers with chromosomal instability.
2004,
Pubmed
Williams,
Zwilch, a new component of the ZW10/ROD complex required for kinetochore functions.
2003,
Pubmed
Yunus,
Structure of the Siz/PIAS SUMO E3 ligase Siz1 and determinants required for SUMO modification of PCNA.
2009,
Pubmed
Yunus,
Lysine activation and functional analysis of E2-mediated conjugation in the SUMO pathway.
2006,
Pubmed
al-Khodairy,
The Schizosaccharomyces pombe hus5 gene encodes a ubiquitin conjugating enzyme required for normal mitosis.
1995,
Pubmed
