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Peptides
2010 Nov 01;3111:2009-16. doi: 10.1016/j.peptides.2010.07.011.
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A new subfamily of conotoxins belonging to the A-superfamily.
Peng C
,
Ye M
,
Wang Y
,
Shao X
,
Yuan D
,
Liu J
,
Hawrot E
,
Wang C
,
Chi C
.
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Two novel conotoxins from vermivorous cone snails Conus pulicarius and Conus tessulatus, designated as Pu14.1 and ts14a, were identified by cDNA cloning and peptide purification, respectively. The signal sequence of Pu14.1 is identical to that of α-conotoxins, while its predicted mature peptide, pu14a, shares high sequence similarity with ts14a, with only one residue different in their first intercysteine loop, which contains 10 residues and is rich in proline. Both pu14a and ts14a contain four separate cysteines in framework 14 (C-C-C-C). Peptide pu14a was chemically synthesized, air oxidized, and the connectivity of its two disulfide bonds was determined to be C1-C3, C2-C4, which is the same as found in α-conotoxins. The synthetic pu14a induced a sleeping symptom in mice and was toxic to freshwater goldfish upon intramuscular injection. Using the Xenopus oocyte heterologous expression system, 1μM of pu14a demonstrated to inhibit the rat neuronal α3β2-containing as well as the mouse neuromuscular α1β1γδ subtypes of nicotinic acetylcholine receptors, and then rapidly dissociated from the receptors. However, this toxin had no inhibitory effect on potassium channels in mouse superior cervical ganglion neurons. According to the identical signal sequence to α-conotoxins, the unique cysteine framework and molecular target of pu14a, we propose that pu14a and ts14a may represent a novel subfamily in the A-superfamily, designated as α1-conotoxins.
Chagot,
An unusual fold for potassium channel blockers: NMR structure of three toxins from the scorpion Opisthacanthus madagascariensis.
2005, Pubmed
Chagot,
An unusual fold for potassium channel blockers: NMR structure of three toxins from the scorpion Opisthacanthus madagascariensis.
2005,
Pubmed
Chi,
Solution conformation of alphaA-conotoxin EIVA, a potent neuromuscular nicotinic acetylcholine receptor antagonist from Conus ermineus.
2003,
Pubmed
Cotton,
A potassium-channel toxin from the sea anemone Bunodosoma granulifera, an inhibitor for Kv1 channels. Revision of the amino acid sequence, disulfide-bridge assignment, chemical synthesis, and biological activity.
1997,
Pubmed
,
Xenbase
Fajloun,
Chemical synthesis and characterization of Pi1, a scorpion toxin from Pandinus imperator active on K+ channels.
2000,
Pubmed
,
Xenbase
Gerzanich,
Homomers of alpha 8 and alpha 7 subunits of nicotinic receptors exhibit similar channel but contrasting binding site properties.
1994,
Pubmed
,
Xenbase
Han,
Characterization of novel M-superfamily conotoxins with new disulfide linkage.
2006,
Pubmed
Han,
NMR structure determination of a novel conotoxin, [Pro 7,13] alpha A-conotoxin PIVA.
1997,
Pubmed
Hopkins,
A new family of Conus peptides targeted to the nicotinic acetylcholine receptor.
1995,
Pubmed
Imperial,
A novel conotoxin inhibitor of Kv1.6 channel and nAChR subtypes defines a new superfamily of conotoxins.
2006,
Pubmed
Jacobsen,
Differential targeting of nicotinic acetylcholine receptors by novel alphaA-conotoxins.
1997,
Pubmed
,
Xenbase
Kaas,
ConoServer, a database for conopeptide sequences and structures.
2008,
Pubmed
Liu,
Two potent alpha3/5 conotoxins from piscivorous Conus achatinus.
2007,
Pubmed
,
Xenbase
Möller,
A novel conotoxin framework with a helix-loop-helix (Cs alpha/alpha) fold.
2005,
Pubmed
Mouhat,
The 'functional' dyad of scorpion toxin Pi1 is not itself a prerequisite for toxin binding to the voltage-gated Kv1.2 potassium channels.
2004,
Pubmed
,
Xenbase
Nirthanan,
Assignment of voltage-gated potassium channel blocking activity to kappa-KTx1.3, a non-toxic homologue of kappa-hefutoxin-1, from Heterometrus spinifer venom.
2005,
Pubmed
Olivera,
Diversity of Conus neuropeptides.
1990,
Pubmed
Peng,
alpha4/7-conotoxin Lp1.1 is a novel antagonist of neuronal nicotinic acetylcholine receptors.
2008,
Pubmed
,
Xenbase
Peng,
Identification of a novel class of conotoxins defined as V-conotoxins with a unique cysteine pattern and signal peptide sequence.
2008,
Pubmed
Peng,
Discovery of a novel class of conotoxin from Conus litteratus, lt14a, with a unique cysteine pattern.
2006,
Pubmed
Sanders,
A novel pharmatope tag inserted into the beta4 subunit confers allosteric modulation to neuronal nicotinic receptors.
2004,
Pubmed
,
Xenbase
Santos,
The A-superfamily of conotoxins: structural and functional divergence.
2004,
Pubmed
Srinivasan,
kappa-Hefutoxin1, a novel toxin from the scorpion Heterometrus fulvipes with unique structure and function. Importance of the functional diad in potassium channel selectivity.
2002,
Pubmed
Teichert,
AlphaA-Conotoxin OIVA defines a new alphaA-conotoxin subfamily of nicotinic acetylcholine receptor inhibitors.
2004,
Pubmed
Teichert,
Discovery and characterization of the short kappaA-conotoxins: a novel subfamily of excitatory conotoxins.
2007,
Pubmed
,
Xenbase
Terlau,
Conus venoms: a rich source of novel ion channel-targeted peptides.
2004,
Pubmed
Verdier,
Identification of a novel pharmacophore for peptide toxins interacting with K+ channels.
2005,
Pubmed
,
Xenbase
Wang,
A novel short-chain peptide BmKX from the Chinese scorpion Buthus martensi Karsch, sequencing, gene cloning and structure determination.
2005,
Pubmed
Yuan,
From the identification of gene organization of alpha conotoxins to the cloning of novel toxins.
2007,
Pubmed
Zugasti-Cruz,
Two new 4-Cys conotoxins (framework 14) of the vermivorous snail Conus austini from the Gulf of Mexico with activity in the central nervous system of mice.
2008,
Pubmed
