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Lamins are nuclear intermediate filament proteins. They are involved in most nuclear activities and are essential for retaining the mechano-elastic properties of the nucleus. Somatic cells of vertebrates express lamins A, B1 and B2 while lamin LIII, a major component of the amphibian oocyte lamina is absent in mammals. The organization of the lamina of germ cells differs significantly from that of somatic cells. Mammalian spermatogenic cells express two short lamins, C2 and B3, that are splice isoforms of lamin A and B2, respectively. Here we identify the previously described Xenopus lamin LIV as splice variant of the lamin LIII gene. LIV contains 40 extra residues in coil 2A of the rod domain, which results in altered assembly properties. Xenopus lamin LIV and mammalian B3 assemble into short structures rather than into long IF-like filaments. Expression of lamin LIV is restricted to male germ cells suggesting that it might be the functional equivalent of mammalian lamin B3. We provide evidence that lamins C2 and B3 are restricted to the mammalian lineage and describe the lamin composition of Xenopus sperm. Our results show that the evolution of germ cell-specific lamins followed separate and distinctly different paths in amphibians and mammals.
Alsheimer,
The dance floor of meiosis: evolutionary conservation of nuclear envelope attachment and dynamics of meiotic telomeres.
2009, Pubmed
Alsheimer,
The dance floor of meiosis: evolutionary conservation of nuclear envelope attachment and dynamics of meiotic telomeres.
2009,
Pubmed
Alsheimer,
Nuclear envelope remodelling during rat spermiogenesis: distribution and expression pattern of LAP2/thymopoietins.
1998,
Pubmed
Alsheimer,
Disruption of spermatogenesis in mice lacking A-type lamins.
2004,
Pubmed
Alsheimer,
Meiotic lamin C2: the unique amino-terminal hexapeptide GNAEGR is essential for nuclear envelope association.
2000,
Pubmed
Alsheimer,
Architecture of the nuclear periphery of rat pachytene spermatocytes: distribution of nuclear envelope proteins in relation to synaptonemal complex attachment sites.
1999,
Pubmed
Alsheimer,
Change of karyoskeleton during mammalian spermatogenesis: expression pattern of nuclear lamin C2 and its regulation.
1996,
Pubmed
Aparicio,
Whole-genome shotgun assembly and analysis of the genome of Fugu rubripes.
2002,
Pubmed
Ashery-Padan,
Localization and posttranslational modifications of otefin, a protein required for vesicle attachment to chromatin, during Drosophila melanogaster development.
1997,
Pubmed
Benavente,
Change of karyoskeleton during spermatogenesis of Xenopus: expression of lamin LIV, a nuclear lamina protein specific for the male germ line.
1985,
Pubmed
,
Xenbase
Ben-Harush,
The supramolecular organization of the C. elegans nuclear lamin filament.
2009,
Pubmed
Bernardini,
Spermiogenesis in Xenopus laevis: from late spermatids to spermatozoa.
1990,
Pubmed
,
Xenbase
Boguski,
dbEST--database for "expressed sequence tags".
1993,
Pubmed
Bowes,
Xenbase: a Xenopus biology and genomics resource.
2008,
Pubmed
,
Xenbase
Döring,
Gene structure of nuclear lamin LIII of Xenopus laevis; a model for the evolution of IF proteins from a lamin-like ancestor.
1990,
Pubmed
,
Xenbase
Fisher,
cDNA sequencing of nuclear lamins A and C reveals primary and secondary structural homology to intermediate filament proteins.
1986,
Pubmed
Fuchs,
Intermediate filaments: structure, dynamics, function, and disease.
1994,
Pubmed
Furukawa,
Identification and cloning of an mRNA coding for a germ cell-specific A-type lamin in mice.
1994,
Pubmed
Furukawa,
cDNA cloning of a germ cell specific lamin B3 from mouse spermatocytes and analysis of its function by ectopic expression in somatic cells.
1993,
Pubmed
Geisler,
Assembly and architecture of invertebrate cytoplasmic intermediate filaments reconcile features of vertebrate cytoplasmic and nuclear lamin-type intermediate filaments.
1998,
Pubmed
Goldberg,
A new model for nuclear lamina organization.
2008,
Pubmed
,
Xenbase
Goldberg,
Filaments made from A- and B-type lamins differ in structure and organization.
2008,
Pubmed
,
Xenbase
Heitlinger,
Expression of chicken lamin B2 in Escherichia coli: characterization of its structure, assembly, and molecular interactions.
1991,
Pubmed
Heitlinger,
The role of the head and tail domain in lamin structure and assembly: analysis of bacterially expressed chicken lamin A and truncated B2 lamins.
1992,
Pubmed
Herrmann,
Intermediate filaments: molecular structure, assembly mechanism, and integration into functionally distinct intracellular Scaffolds.
2004,
Pubmed
Izumi,
Head and/or CaaX domain deletions of lamin proteins disrupt preformed lamin A and C but not lamin B structure in mammalian cells.
2000,
Pubmed
,
Xenbase
Jaillon,
Genome duplication in the teleost fish Tetraodon nigroviridis reveals the early vertebrate proto-karyotype.
2004,
Pubmed
Karabinos,
The single nuclear lamin of Caenorhabditis elegans forms in vitro stable intermediate filaments and paracrystals with a reduced axial periodicity.
2003,
Pubmed
Klapper,
Assembly of A- and B-type lamins studied in vivo with the baculovirus system.
1997,
Pubmed
,
Xenbase
Lehner,
Differential expression of nuclear lamin proteins during chicken development.
1987,
Pubmed
,
Xenbase
Liu,
Essential roles for Caenorhabditis elegans lamin gene in nuclear organization, cell cycle progression, and spatial organization of nuclear pore complexes.
2000,
Pubmed
Lupas,
Predicting coiled coils from protein sequences.
1991,
Pubmed
Moir,
Disruption of nuclear lamin organization blocks the elongation phase of DNA replication.
2000,
Pubmed
,
Xenbase
Nakajima,
Genomic structure of the mouse A-type lamin gene locus encoding somatic and germ cell-specific lamins.
1995,
Pubmed
Ouali,
Cascaded multiple classifiers for secondary structure prediction.
2000,
Pubmed
Peter,
Ectopic expression of prelamin A in early Xenopus embryos induces apoptosis.
2008,
Pubmed
,
Xenbase
Prokocimer,
Nuclear lamins: key regulators of nuclear structure and activities.
2009,
Pubmed
Scherthan,
A bouquet makes ends meet.
2001,
Pubmed
Schütz,
Nuclear envelope remodeling during mouse spermiogenesis: postmeiotic expression and redistribution of germline lamin B3.
2005,
Pubmed
Schütz,
Dynamic properties of germ line-specific lamin B3: the role of the shortened rod domain.
2005,
Pubmed
Shumaker,
The highly conserved nuclear lamin Ig-fold binds to PCNA: its role in DNA replication.
2008,
Pubmed
,
Xenbase
Smith,
Identification of a short nuclear lamin protein selectively expressed during meiotic stages of rat spermatogenesis.
1992,
Pubmed
Spann,
Disruption of nuclear lamin organization alters the distribution of replication factors and inhibits DNA synthesis.
1997,
Pubmed
,
Xenbase
Stick,
The gene structure of B-type nuclear lamins of Xenopus laevis: implications for the evolution of the vertebrate lamin family.
1994,
Pubmed
,
Xenbase
Stick,
cDNA cloning of the developmentally regulated lamin LIII of Xenopus laevis.
1988,
Pubmed
,
Xenbase
Stuurman,
Nuclear lamins: their structure, assembly, and interactions.
1998,
Pubmed
Stuurman,
Intermediate filament protein polymerization: molecular analysis of Drosophila nuclear lamin head-to-tail binding.
1996,
Pubmed
Vester,
Presence of a nuclear lamina in pachytene spermatocytes of the rat.
1993,
Pubmed
Wolin,
A new lamin in Xenopus somatic tissues displays strong homology to human lamin A.
1987,
Pubmed
,
Xenbase
Zewe,
Gene structure and chromosomal localization of the murine lamin B2 gene.
1991,
Pubmed
,
Xenbase
Zimek,
Terrestrial vertebrates have two keratin gene clusters; striking differences in teleost fish.
2005,
Pubmed
,
Xenbase
