Kaeser GE , Rabe BA , Saha MS .

R15 NS067566-01 NINDS NIH HHS , Howard Hughes Medical Institute , R15 NS067566 NINDS NIH HHS

	Figure 2. Expression patterns of GABAA receptor α-subunits and GABAB receptors in developing X. laevis embryos using whole mount in situ hybridization. Embryos were viewed laterally with anterior to the left and dorsal to the top. The arrowheads indicate regions of expression. A, D, G, J, M, P, S: Stages 24–26, (tailbud stages). B, E, H, K, N, Q, T: Stage 29–31 (hatching stages). C, F, I, L, O, R, U: Stages 34–36 (early swimming tadpole stages). A–C: GABAAα1 expression. D–F: GABAAα2 expression. G–I: GABAAα3 expression. J–L: GABAAα5 expression. M–O: GABAAα6 expression. P-R: GABAB1 expression. S–U: GABAB2 expression. F′, H′, I′, P′, Q′, R′, S′, T′, U′: Magnified images of portions of embryos. fb, forebrain; r, retina; hb, hindbrain; mb, midbrain; pg, pineal gland; sc, spinal cord; V, cranial ganglion V; VII, cranial ganglion VII; IX, cranial ganglion IX. Scale bar = 1.0 mm; scale bar in inset = 0.25 mm.Download figure to PowerPoint
	Figure 3. Histological analysis of GABA receptor subunit expression in swimming tadpole (stage 35) X. laevis embryos. Transverse 18-μm sections along the anterior-posterior axis, with dorsal to the top. A–E: GABAAα1 expression. F–J: GABAAα2 expression. K–O: GABAAα3 expression. P–T: GABAAα5 expression. U–Y: GABAB1 expression. Z-DD: GABAB2 expression. Prime letters: Magnified portions of sections. W′: Paraffin section to show lack of otic vesicle staining. fb, forebrain; mb, midbrain; hb, hindbrain; asc, anterior spinal cord; psc, posterior spinal cord; r, retina; sc, spinal cord; V, cranial ganglion V; VII, cranial ganglion VII,; IX, cranial ganglion IX: oe; olfactory epithelium; Pt, pituitary gland. Scale bars = 0.3 mm.Download figure to PowerPoint
	Figure 4. Retinal histological analysis of GABA receptor subunit expression in swimming tadpole (stage 40) X. laevis embryos. Transverse 18-μm sections of the retina, with dorsal to the top. Brackets, retinal layers with expression; black triangles, ganglion cells; white triangles, horizontal cells; black arrows, amacrine cells. A: GABAAα1 expression. B: GABAAα2 expression. C: GABAAα6 expression (*stage 35). D: GABAB1 expression. E: GABAB2 expression. GCL, ganglion cell layer; INL, inner nuclear layer; L, lens; PR, photoreceptor layer. Scale bar = 0.1 mm.Download figure to PowerPoint
	Figure 5. Early Expression of GABAA and GABAB receptors in X. laevis embryos. A: GABAAα2 expression at blastula stage (stage 6). Transverse 15-μm sections parallel to animal-vegetal axis, with the animal pole to the top. Arrowheads, regions of expression. B: GABAB1 expression at stage 20–22. Embryo was viewed laterally with anterior to the left and dorsal to the top. The arrowheads indicate regions of expression. fb, forebrain; mb, midbrain. Scale bars = 0.25 mm in A; 0.4 mm in B.Download figure to PowerPoint
	gabbr1 [GABA B receptor 1] gene expression in Xenopus laevis embryo via in situ hybridization, NF stage (24-26), lateral view, anterior left, dorsal up. Image copyrighted by: Wiley-Liss Inc and reproduced from Kaeser et al., XB-ART-42885
	gabbr1 [GABA B receptor 1] gene expression in Xenopus laevis embryo via in situ hybridization, NF stage (29-31), lateral view, anterior left, dorsal up. Image copyrighted by: Wiley-Liss Inc and reproduced from Kaeser et al., XB-ART-42885
	gabbr1 [GABA B receptor 1] gene expression in Xenopus laevis embryo via in situ hybridization, NF stage (34-36), lateral view, anterior left, dorsal up. Image copyrighted by: Wiley-Liss Inc and reproduced from Kaeser et al., XB-ART-42885
	gabbr2[GABA B receptor 2] gene expression in Xenopus laevis embryo via in situ hybridization, NF stage (24-26), lateral view, anterior left, dorsal up. Image copyrighted by: Wiley-Liss Inc and reproduced from Kaeser et al., XB-ART-42885
	gabbr2 [GABA B receptor 2] gene expression in Xenopus laevis embryo via in situ hybridization, NF stage (29-31), lateral view, anterior left, dorsal up. Image copyrighted by: Wiley-Liss Inc and reproduced from Kaeser et al., XB-ART-42885
	gabbr2 [GABA B receptor 2] gene expression in Xenopus laevis embryo via in situ hybridization, NF stage (34-36), lateral view, anterior left, dorsal up. Image copyrighted by: Wiley-Liss Inc and reproduced from Kaeser et al., XB-ART-42885
	gabra2 [GABA a receptor 2] gene expression in Xenopus laevis embryo via in situ hybridization, NF stage (29-31), lateral view, anterior left, dorsal up. Image copyrighted by: Wiley-Liss Inc and reproduced from Kaeser et al., XB-ART-42885
	gabra2 [GABA A receptor, alpha 2] gene expression in Xenopus laevis embryo via in situ hybridization, NF stage (34-36), lateral view, anterior left, dorsal up. Image copyrighted by: Wiley-Liss Inc and reproduced from Kaeser et al., XB-ART-42885
	gabra2 [GABA A receptor, alpha 2] gene expression in Xenopus laevis embryo via in situ hybridization, NF stage (24-26), lateral view, anterior left, dorsal up. Image copyrighted by: Wiley-Liss Inc and reproduced from Kaeser et al., XB-ART-42885
	gabra2 [GABA A receptor, alpha 2] gene expression in Xenopus laevis embryo via in situ hybridization, NF stage (29-31), lateral view, anterior left, dorsal up. Image copyrighted by: Wiley-Liss Inc and reproduced from Kaeser et al., XB-ART-42885
	gabra2 [GABA A receptor, alpha 2] gene expression in Xenopus laevis embryo via in situ hybridization, NF stage (34-36), lateral view, anterior left, dorsal up. Image copyrighted by: Wiley-Liss Inc and reproduced from Kaeser et al., XB-ART-42885
	gabrg3 [gamma-aminobutyric acid (GABA) A receptor, gamma 3] gene expression in Xenopus laevis embryo via in situ hybridization, NF stage (29-31), lateral view, anterior left, dorsal up. Image copyrighted by: Wiley-Liss Inc and reproduced from Kaeser et al., XB-ART-42885
	gabrg3 [gamma-aminobutyric acid (GABA) A receptor, gamma 3] gene expression in Xenopus laevis embryo via in situ hybridization, NF stage (34-36), lateral view, anterior left, dorsal up. Image copyrighted by: Wiley-Liss Inc and reproduced from Kaeser et al., XB-ART-42885
	gabra1 [gamma-aminobutyric acid (GABA) A receptor, alpha 1] gene expression in Xenopus laevis embryo via in situ hybridization, NF stage (24-26), lateral view, anterior left, dorsal up. Image copyrighted by: Wiley-Liss Inc and reproduced from Kaeser et al., XB-ART-42885
	gabra1 [gamma-aminobutyric acid (GABA) A receptor, alpha 1] gene expression in Xenopus laevis embryo via in situ hybridization, NF stage (29-31), lateral view, anterior left, dorsal up. Image copyrighted by: Wiley-Liss Inc and reproduced from Kaeser et al., XB-ART-42885
	gabra1 [gamma-aminobutyric acid (GABA) A receptor, alpha 1] gene expression in Xenopus laevis embryo via in situ hybridization, NF stage (34-36), lateral view, anterior left, dorsal up. Image copyrighted by: Wiley-Liss Inc and reproduced from Kaeser et al., XB-ART-42885
	gabra5 [gamma-aminobutyric acid (GABA) A receptor, alpha 5] gene expression in Xenopus laevis embryo via in situ hybridization, NF stage (34-36), lateral view, anterior left, dorsal up. Image copyrighted by: Wiley-Liss Inc and reproduced from Kaeser et al., XB-ART-42885

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