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J Biol Chem
2011 May 13;28619:16953-7. doi: 10.1074/jbc.M111.224576.
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Conformational changes at the agonist binding domain of the N-methyl-D-aspartic acid receptor.
Rambhadran A
,
Gonzalez J
,
Jayaraman V
.
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The conformational changes in the agonist binding domain of the glycine-binding GluN1 and glutamate-binding GluN2A subunits of the N-methyl D-aspartic acid receptor upon binding agonists of varying efficacy have been investigated by luminescence resonance energy transfer (LRET) measurements. The LRET-based distances indicate a cleft closure conformational change at the GluN1 subunit upon binding agonists; however, no significant changes in the cleft closure are observed between partial and full agonists. This is consistent with the previously reported crystal structures for the isolated agonist binding domain of this receptor. Additionally, the LRET-based distances show that the agonist binding domain of the glutamate-binding GluN2A subunit exhibits a graded cleft closure with the extent of cleft closure being proportional to the extent of activation, indicating that the mechanism of activation in this subunit is similar to that of the glutamate binding α-amino-5-methyl-3-hydroxy-4-isoxazole propionate and kainate subtypes of the ionotropic glutamate receptors.
Ahmed,
Dynamics of the S1S2 glutamate binding domain of GluR2 measured using 19F NMR spectroscopy.
2007, Pubmed
Ahmed,
Dynamics of the S1S2 glutamate binding domain of GluR2 measured using 19F NMR spectroscopy.
2007,
Pubmed
Armstrong,
Measurement of conformational changes accompanying desensitization in an ionotropic glutamate receptor.
2006,
Pubmed
,
Xenbase
Armstrong,
Mechanisms for activation and antagonism of an AMPA-sensitive glutamate receptor: crystal structures of the GluR2 ligand binding core.
2000,
Pubmed
Benveniste,
Kinetic analysis of antagonist action at N-methyl-D-aspartic acid receptors. Two binding sites each for glutamate and glycine.
1991,
Pubmed
Cheng,
A vibrational spectroscopic investigation of interactions of agonists with GluR0, a prokaryotic glutamate receptor.
2002,
Pubmed
Cheng,
Evolution of glutamate interactions during binding to a glutamate receptor.
2005,
Pubmed
Cheng,
Chemistry and conformation of the ligand-binding domain of GluR2 subtype of glutamate receptors.
2004,
Pubmed
Clements,
Activation kinetics reveal the number of glutamate and glycine binding sites on the N-methyl-D-aspartate receptor.
1991,
Pubmed
Deming,
Is the isolated ligand binding domain a good model of the domain in the native receptor?
2003,
Pubmed
Du,
Conformational changes in the ligand-binding domain of a functional ionotropic glutamate receptor.
2005,
Pubmed
Erreger,
Subunit-specific agonist activity at NR2A-, NR2B-, NR2C-, and NR2D-containing N-methyl-D-aspartate glutamate receptors.
2007,
Pubmed
,
Xenbase
Fenwick,
NMR spectroscopy of the ligand-binding core of ionotropic glutamate receptor 2 bound to 5-substituted willardiine partial agonists.
2008,
Pubmed
Furukawa,
Subunit arrangement and function in NMDA receptors.
2005,
Pubmed
Furukawa,
Mechanisms of activation, inhibition and specificity: crystal structures of the NMDA receptor NR1 ligand-binding core.
2003,
Pubmed
Gonzalez,
LRET investigations of conformational changes in the ligand binding domain of a functional AMPA receptor.
2008,
Pubmed
,
Xenbase
Inanobe,
Mechanism of partial agonist action at the NR1 subunit of NMDA receptors.
2005,
Pubmed
,
Xenbase
Jayaraman,
Spectroscopic and kinetic methods for ligand-protein interactions of glutamate receptor.
2004,
Pubmed
Jayaraman,
Ligand--protein interactions in the glutamate receptor.
2000,
Pubmed
Johnson,
Glycine potentiates the NMDA response in cultured mouse brain neurons.
,
Pubmed
Landes,
Structural landscape of isolated agonist-binding domains from single AMPA receptors.
2011,
Pubmed
Madden,
Stereochemistry of glutamate receptor agonist efficacy: engineering a dual-specificity AMPA/kainate receptor.
2004,
Pubmed
Madden,
Stereochemistry of quinoxaline antagonist binding to a glutamate receptor investigated by Fourier transform infrared spectroscopy.
2001,
Pubmed
Maltsev,
Hydrophobic side chain dynamics of a glutamate receptor ligand binding domain.
2010,
Pubmed
Maltsev,
Mechanism of partial agonism at the GluR2 AMPA receptor: Measurements of lobe orientation in solution.
2008,
Pubmed
Mankiewicz,
Glutamate receptors as seen by light: spectroscopic studies of structure-function relationships.
2007,
Pubmed
Mankiewicz,
Chemical interplay in the mechanism of partial agonist activation in alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptors.
2008,
Pubmed
Mankiewicz,
Role of the chemical interactions of the agonist in controlling alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor activation.
2007,
Pubmed
Mayer,
Crystal structures of the GluR5 and GluR6 ligand binding cores: molecular mechanisms underlying kainate receptor selectivity.
2005,
Pubmed
Mayer,
Crystal structures of the kainate receptor GluR5 ligand binding core dimer with novel GluR5-selective antagonists.
2006,
Pubmed
,
Xenbase
Mayer,
Structure and function of glutamate receptor ion channels.
2004,
Pubmed
McFeeters,
Emerging structural explanations of ionotropic glutamate receptor function.
2004,
Pubmed
McFeeters,
Structural mobility of the extracellular ligand-binding core of an ionotropic glutamate receptor. Analysis of NMR relaxation dynamics.
2002,
Pubmed
Nanao,
Structure of the kainate receptor subunit GluR6 agonist-binding domain complexed with domoic acid.
2005,
Pubmed
Naur,
Crystal structure of the kainate receptor GluR5 ligand-binding core in complex with (S)-glutamate.
2005,
Pubmed
Ramanoudjame,
Allosteric mechanism in AMPA receptors: a FRET-based investigation of conformational changes.
2006,
Pubmed
Rambhadran,
Subunit arrangement in N-methyl-D-aspartate (NMDA) receptors.
2010,
Pubmed
Sobolevsky,
X-ray structure, symmetry and mechanism of an AMPA-subtype glutamate receptor.
2009,
Pubmed
Sun,
Mechanism of glutamate receptor desensitization.
2002,
Pubmed
