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Biophys J
2011 May 18;10010:2387-93. doi: 10.1016/j.bpj.2011.01.073.
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A multipoint hydrogen-bond network underlying KcsA C-type inactivation.
Cordero-Morales JF
,
Jogini V
,
Chakrapani S
,
Perozo E
.
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In the prokaryotic potassium channel KcsA activation gating at the inner bundle gate is followed by C-type inactivation at the selectivity filter. Entry into the C-type inactivated state has been directly linked to the strength of the H-bond interaction between residues Glu-71 and Asp-80 behind the filter, and is allosterically triggered by the rearrangement of the inner bundle gate. Here, we show that H-bond pairing between residues Trp-67 and Asp-80, conserved in most K⁺ channels, constitutes another critical interaction that determines the rate and extent of KcsA C-type inactivation. Disruption of the equivalent interaction in Shaker (Trp-434-Asp-447) and Kv1.2 (Trp-366-Asp-379) leads also to modulation of the inactivation process, suggesting that these residues also play an analogous role in the inactivation gating of Kv channels. The present results show that in KcsA C-type inactivation gating is governed by a multipoint hydrogen-bond network formed by the triad Trp-67-Glu71-Asp-80. This triad exerts a critical role in the dynamics and conformational stability of the selectivity filter and might serve as a general modulator of selectivity filter gating in other members of the K⁺ channel family.
Alagem,
The pore helix is involved in stabilizing the open state of inwardly rectifying K+ channels.
2003, Pubmed,
Xenbase
Alagem,
The pore helix is involved in stabilizing the open state of inwardly rectifying K+ channels.
2003,
Pubmed
,
Xenbase
Baker,
Conformational dynamics of the KcsA potassium channel governs gating properties.
2007,
Pubmed
Baukrowitz,
Modulation of K+ current by frequency and external [K+]: a tale of two inactivation mechanisms.
1995,
Pubmed
Bernèche,
Molecular dynamics of the KcsA K(+) channel in a bilayer membrane.
2000,
Pubmed
Bezanilla,
Negative conductance caused by entry of sodium and cesium ions into the potassium channels of squid axons.
1972,
Pubmed
Chakrapani,
A quantitative description of KcsA gating I: macroscopic currents.
2007,
Pubmed
Chapman,
Allosteric effects of external K+ ions mediated by the aspartate of the GYGD signature sequence in the Kv2.1 K+ channel.
2006,
Pubmed
,
Xenbase
Cordero-Morales,
Molecular driving forces determining potassium channel slow inactivation.
2007,
Pubmed
,
Xenbase
Cordero-Morales,
Molecular determinants of gating at the potassium-channel selectivity filter.
2006,
Pubmed
Cortes,
Molecular architecture of full-length KcsA: role of cytoplasmic domains in ion permeation and activation gating.
2001,
Pubmed
Cortes,
Structural dynamics of the Streptomyces lividans K+ channel (SKC1): oligomeric stoichiometry and stability.
1997,
Pubmed
,
Xenbase
Cuello,
Structural mechanism of C-type inactivation in K(+) channels.
2010,
Pubmed
Cuello,
Structural basis for the coupling between activation and inactivation gates in K(+) channels.
2010,
Pubmed
Delcour,
Modified reconstitution method used in patch-clamp studies of Escherichia coli ion channels.
1989,
Pubmed
Doyle,
The structure of the potassium channel: molecular basis of K+ conduction and selectivity.
1998,
Pubmed
Ficker,
Molecular determinants of dofetilide block of HERG K+ channels.
1998,
Pubmed
,
Xenbase
Heginbotham,
Mutations in the K+ channel signature sequence.
1994,
Pubmed
,
Xenbase
Hoshi,
Biophysical and molecular mechanisms of Shaker potassium channel inactivation.
1990,
Pubmed
,
Xenbase
Hurst,
Molecular determinants of external barium block in Shaker potassium channels.
1996,
Pubmed
,
Xenbase
Imai,
Structural basis underlying the dual gate properties of KcsA.
2010,
Pubmed
Jiang,
The open pore conformation of potassium channels.
2002,
Pubmed
Kiss,
Contribution of the selectivity filter to inactivation in potassium channels.
1999,
Pubmed
Lange,
Toxin-induced conformational changes in a potassium channel revealed by solid-state NMR.
2006,
Pubmed
,
Xenbase
Liu,
Gated access to the pore of a voltage-dependent K+ channel.
1997,
Pubmed
Liu,
Dynamic rearrangement of the outer mouth of a K+ channel during gating.
1996,
Pubmed
Lockless,
Structural and thermodynamic properties of selective ion binding in a K+ channel.
2007,
Pubmed
Long,
Crystal structure of a mammalian voltage-dependent Shaker family K+ channel.
2005,
Pubmed
Loots,
Protein rearrangements underlying slow inactivation of the Shaker K+ channel.
1998,
Pubmed
Marius,
Binding of anionic lipids to at least three nonannular sites on the potassium channel KcsA is required for channel opening.
2008,
Pubmed
Nishida,
Crystal structure of a Kir3.1-prokaryotic Kir channel chimera.
2007,
Pubmed
Peng,
Hydropathic analysis and comparison of KcsA and Shaker potassium channels.
2007,
Pubmed
Perozo,
Gating currents from a nonconducting mutant reveal open-closed conformations in Shaker K+ channels.
1993,
Pubmed
Perozo,
Three-dimensional architecture and gating mechanism of a K+ channel studied by EPR spectroscopy.
1998,
Pubmed
Proks,
Mutations within the P-loop of Kir6.2 modulate the intraburst kinetics of the ATP-sensitive potassium channel.
2001,
Pubmed
,
Xenbase
Rotem,
Inactivation of the KcsA potassium channel explored with heterotetramers.
2010,
Pubmed
Shealy,
Sequence-function analysis of the K+-selective family of ion channels using a comprehensive alignment and the KcsA channel structure.
2003,
Pubmed
Smith,
The inward rectification mechanism of the HERG cardiac potassium channel.
1996,
Pubmed
Valiyaveetil,
Structural and functional consequences of an amide-to-ester substitution in the selectivity filter of a potassium channel.
2006,
Pubmed
Yang,
How does the W434F mutation block current in Shaker potassium channels?
1997,
Pubmed
,
Xenbase
Yifrach,
Energetics of pore opening in a voltage-gated K(+) channel.
2002,
Pubmed
,
Xenbase
Zhou,
Chemistry of ion coordination and hydration revealed by a K+ channel-Fab complex at 2.0 A resolution.
2001,
Pubmed
