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Nucleus
2010 Jan 01;11:109-22. doi: 10.4161/nucl.1.1.10857.
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Novel domains in the hnRNP G/RBMX protein with distinct roles in RNA binding and targeting nascent transcripts.
Kanhoush R
,
Beenders B
,
Perrin C
,
Moreau J
,
Bellini M
,
Penrad-Mobayed M
.
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The heterogenous nuclear ribonucleoprotein G (hnRNP G) controls the alternative splicing of several pre-mRNas. While hnRNP G displays an amino terminal RNA recognition motif (RRM), we find that this motif is paradoxically not implicated in the recruitment of hnRNP G to nascent transcripts in amphibian oocytes. In fact, a deletion analysis revealed that targeting of hnRNP G to active transcription units depends on another domain, centrally positioned, and consisting of residues 186-236. We show that this domain acts autonomously and thus is named NTD for nascent transcripts targeting domain. Furthermore, using an RNA probe previously characterized in vitro as an RNA that interacts specifically with hnRNP G, we demonstrate a new auxiliary RNA binding domain (RBD). It corresponds to a short region of 58 residues positioned at the carboxyl terminal end of the protein, which recognizes an RNA motif predicted to adopt an hairpin structure. The fact that the NTD acts independently from both the RRM and the RBD strongly suggests that the initial recruitment of hnRNP G to nascent pre-mRNAs is independent of its sequence-specific RNA binding properties. Together, these findings highlight the modular organization of hnRNP G and offer new insights into its multifunctional roles.
Austin,
Lampbrush chromosomes enable study of cohesin dynamics.
2009, Pubmed,
Xenbase
Austin,
Lampbrush chromosomes enable study of cohesin dynamics.
2009,
Pubmed
,
Xenbase
Beenders,
The tripartite motif of nuclear factor 7 is required for its association with transcriptional units.
2007,
Pubmed
,
Xenbase
Bellini,
Coilin can form a complex with the U7 small nuclear ribonucleoprotein.
1998,
Pubmed
,
Xenbase
Bentley,
Rules of engagement: co-transcriptional recruitment of pre-mRNA processing factors.
2005,
Pubmed
Cooper,
RNA and disease.
2009,
Pubmed
Darty,
VARNA: Interactive drawing and editing of the RNA secondary structure.
2009,
Pubmed
Delbridge,
The candidate spermatogenesis gene RBMY has a homologue on the human X chromosome.
1999,
Pubmed
Dichmann,
Expression cloning in Xenopus identifies RNA-binding proteins as regulators of embryogenesis and Rbmx as necessary for neural and muscle development.
2008,
Pubmed
,
Xenbase
Dreyfuss,
hnRNP proteins and the biogenesis of mRNA.
1993,
Pubmed
Dreyfuss,
Messenger-RNA-binding proteins and the messages they carry.
2002,
Pubmed
Elliott,
The role of potential splicing factors including RBMY, RBMX, hnRNPG-T and STAR proteins in spermatogenesis.
2004,
Pubmed
Gall,
The centennial of the Cajal body.
2003,
Pubmed
Ginisty,
Two different combinations of RNA-binding domains determine the RNA binding specificity of nucleolin.
2001,
Pubmed
,
Xenbase
Glisovic,
RNA-binding proteins and post-transcriptional gene regulation.
2008,
Pubmed
Heinrich,
Heterogeneous nuclear ribonucleoprotein G regulates splice site selection by binding to CC(A/C)-rich regions in pre-mRNA.
2009,
Pubmed
Hofmann,
hnRNP-G promotes exon 7 inclusion of survival motor neuron (SMN) via direct interaction with Htra2-beta1.
2002,
Pubmed
Hung,
Proteomic analysis of methylarginine-containing proteins in HeLa cells by two-dimensional gel electrophoresis and immunoblotting with a methylarginine-specific antibody.
2009,
Pubmed
Iijima,
Two motifs essential for nuclear import of the hnRNP A1 nucleocytoplasmic shuttling sequence M9 core.
2006,
Pubmed
Larkin,
Clustal W and Clustal X version 2.0.
2007,
Pubmed
Lin,
Use of a recombinant protein containing major epitopes of hnRNP G to detect anti-hnRNP G antibodies in dogs with systemic lupus erythematosus.
2006,
Pubmed
Lingenfelter,
Expression and conservation of processed copies of the RBMX gene.
2001,
Pubmed
Martinez-Contreras,
hnRNP proteins and splicing control.
2007,
Pubmed
Mathews,
Expanded sequence dependence of thermodynamic parameters improves prediction of RNA secondary structure.
1999,
Pubmed
Mazeyrat,
RBMY evolved on the Y chromosome from a ubiquitously transcribed X-Y identical gene.
1999,
Pubmed
Morgan,
Lampbrush chromosomes and associated bodies: new insights into principles of nuclear structure and function.
2002,
Pubmed
Murphy,
RNA polymerase III in Cajal bodies and lampbrush chromosomes of the Xenopus oocyte nucleus.
2002,
Pubmed
,
Xenbase
Nasim,
HnRNP G and Tra2beta: opposite effects on splicing matched by antagonism in RNA binding.
2003,
Pubmed
Oberstrass,
Structure of PTB bound to RNA: specific binding and implications for splicing regulation.
2005,
Pubmed
Penrad-Mobayed,
Microdissection and cloning of DNA from landmark loops of amphibian lampbrush chromosomes.
1991,
Pubmed
Penrad-Mobayed,
Evidence for specific RNA/protein interactions in the differential segment of the W sex chromosome in the amphibian Pleurodeles waltl.
1998,
Pubmed
Sallacz,
Chromosomal storage of the RNA-editing enzyme ADAR1 in Xenopus oocytes.
2005,
Pubmed
,
Xenbase
Scherly,
Major determinants of the specificity of interaction between small nuclear ribonucleoproteins U1A and U2B'' and their cognate RNAs.
1990,
Pubmed
,
Xenbase
Shin,
p53 promotes the fidelity of DNA end-joining activity by, in part, enhancing the expression of heterogeneous nuclear ribonucleoprotein G.
2007,
Pubmed
Shin,
hnRNP G elicits tumor-suppressive activity in part by upregulating the expression of Txnip.
2008,
Pubmed
Siomi,
A nuclear localization domain in the hnRNP A1 protein.
1995,
Pubmed
Skrisovska,
The testis-specific human protein RBMY recognizes RNA through a novel mode of interaction.
2007,
Pubmed
Smith,
Alternative pre-mRNA splicing: the logic of combinatorial control.
2000,
Pubmed
Soulard,
Autoimmune antibodies to hnRNPG protein in dogs with systemic lupus erythematosus: epitope mapping of the antigen.
2002,
Pubmed
Soulard,
A novel 43-kDa glycoprotein is detected in the nucleus of mammalian cells by autoantibodies from dogs with autoimmune disorders.
1991,
Pubmed
Soulard,
hnRNP G: sequence and characterization of a glycosylated RNA-binding protein.
1993,
Pubmed
Takemoto,
RBMX is a novel hepatic transcriptional regulator of SREBP-1c gene response to high-fructose diet.
2007,
Pubmed
Tsend-Ayush,
RBMX gene is essential for brain development in zebrafish.
2005,
Pubmed
Venables,
Multiple and specific mRNA processing targets for the major human hnRNP proteins.
2008,
Pubmed
Venables,
RBMY, a probable human spermatogenesis factor, and other hnRNP G proteins interact with Tra2beta and affect splicing.
2000,
Pubmed
Wallace,
Protein incorporation by isolated amphibian oocytes. 3. Optimum incubation conditions.
1973,
Pubmed
,
Xenbase
Zhao,
Heterogeneous nuclear ribonucleoprotein A/B and G inhibits the transcription of gonadotropin-releasing-hormone 1.
2008,
Pubmed
Zuker,
Mfold web server for nucleic acid folding and hybridization prediction.
2003,
Pubmed
de Almeida,
The CTD role in cotranscriptional RNA processing and surveillance.
2008,
Pubmed
