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Proc Natl Acad Sci U S A
2003 Oct 14;10021:12081-6. doi: 10.1073/pnas.1635192100.
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DcpS can act in the 5'-3' mRNA decay pathway in addition to the 3'-5' pathway.
van Dijk E, Le Hir H, Séraphin B.
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Eukaryotic mRNA degradation proceeds through two main pathways, both involving mRNA cap breakdown. In the 3'-5' mRNA decay pathway, mRNA body degradation generates free m7GpppN that is hydrolyzed by DcpS generating m7GMP. In the 5'-3' pathway, the recently identified human Dcp2 decapping enzyme cleaves the cap of deadenylated mRNAs to produce m7GDP and 5'-phosphorylated mRNA. We investigated mRNA decay in human cell extracts by using a new assay for decapping. We observed that 5'-phosphorylated intermediates resulting from decapping appear after incubation of a substrate RNA in human cell extracts, indicating the presence of an active 5'-3' mRNA decay pathway. Surprisingly, however, the cognate m7GDP product was not detected, whereas abundant amounts of m7GMP were generated. Additional experiments revealed that m7GDP is, unexpectedly, efficiently converted to m7GMP in extracts from various organisms. The factor necessary and sufficient for this reaction was identified as DcpS in both yeast and human. m7GMP is thus a general, pathway-independent, by-product of eukaryotic mRNA decay. m7GDP breakdown should prevent misincorporation of methylated nucleotides in nucleic acids and could generate a unique indicator allowing the cell to monitor mRNA decay.
Anderson,
The 3' to 5' degradation of yeast mRNAs is a general mechanism for mRNA turnover that requires the SKI2 DEVH box protein and 3' to 5' exonucleases of the exosome complex.
1998, Pubmed
Anderson,
The 3' to 5' degradation of yeast mRNAs is a general mechanism for mRNA turnover that requires the SKI2 DEVH box protein and 3' to 5' exonucleases of the exosome complex.
1998,
Pubmed Banerjee,
5'-terminal cap structure in eucaryotic messenger ribonucleic acids.
1980,
Pubmed Bashkirov,
A mouse cytoplasmic exoribonuclease (mXRN1p) with preference for G4 tetraplex substrates.
1997,
Pubmed Beelman,
An essential component of the decapping enzyme required for normal rates of mRNA turnover.
1996,
Pubmed Bergman,
Analysis of the products of mRNA decapping and 3'-to-5' decay by denaturing gel electrophoresis.
2002,
Pubmed Caponigro,
Mechanisms and control of mRNA turnover in Saccharomyces cerevisiae.
1996,
Pubmed Chen,
AU binding proteins recruit the exosome to degrade ARE-containing mRNAs.
2001,
Pubmed Couttet,
Messenger RNA deadenylylation precedes decapping in mammalian cells.
1997,
Pubmed Czaplinski,
Should we kill the messenger? The role of the surveillance complex in translation termination and mRNA turnover.
1999,
Pubmed Daugeron,
The yeast POP2 gene encodes a nuclease involved in mRNA deadenylation.
2001,
Pubmed Dunckley,
The DCP2 protein is required for mRNA decapping in Saccharomyces cerevisiae and contains a functional MutT motif.
1999,
Pubmed Frischmeyer,
An mRNA surveillance mechanism that eliminates transcripts lacking termination codons.
2002,
Pubmed Gao,
A novel mRNA-decapping activity in HeLa cytoplasmic extracts is regulated by AU-rich elements.
2001,
Pubmed Gera,
Deadenylation-dependent and -independent decay pathways for alpha1-tubulin mRNA in Chlamydomonas reinhardtii.
1998,
Pubmed Higgs,
Oat phytochrome A mRNA degradation appears to occur via two distinct pathways.
1994,
Pubmed Hsu,
Yeast cells lacking 5'-->3' exoribonuclease 1 contain mRNA species that are poly(A) deficient and partially lack the 5' cap structure.
1993,
Pubmed Lee,
Identification and characterization of a human cDNA homologous to yeast SKI2.
1995,
Pubmed Lennon,
The I.M.A.G.E. Consortium: an integrated molecular analysis of genomes and their expression.
1996,
Pubmed Lewis,
The influence of 5' and 3' end structures on pre-mRNA metabolism.
1995,
Pubmed Lim,
Human beta-globin mRNAs that harbor a nonsense codon are degraded in murine erythroid tissues to intermediates lacking regions of exon I or exons I and II that have a cap-like structure at the 5' termini.
1992,
Pubmed Liu,
The scavenger mRNA decapping enzyme DcpS is a member of the HIT family of pyrophosphatases.
2002,
Pubmed Lykke-Andersen,
Identification of a human decapping complex associated with hUpf proteins in nonsense-mediated decay.
2002,
Pubmed Mitchell,
mRNA turnover.
2001,
Pubmed Mitchell,
The exosome: a conserved eukaryotic RNA processing complex containing multiple 3'-->5' exoribonucleases.
1997,
Pubmed Mukherjee,
The mammalian exosome mediates the efficient degradation of mRNAs that contain AU-rich elements.
2002,
Pubmed Salgado-Garrido,
Sm and Sm-like proteins assemble in two related complexes of deep evolutionary origin.
1999,
Pubmed Steiger,
Analysis of recombinant yeast decapping enzyme.
2003,
Pubmed Usher,
On the mechanism of ribonuclease action.
1969,
Pubmed van Dijk,
Human Dcp2: a catalytically active mRNA decapping enzyme located in specific cytoplasmic structures.
2002,
Pubmed van Hoof,
Exosome-mediated recognition and degradation of mRNAs lacking a termination codon.
2002,
Pubmed van Hoof,
Messenger RNA degradation: beginning at the end.
2002,
Pubmed Wang,
The hDcp2 protein is a mammalian mRNA decapping enzyme.
2002,
Pubmed Wang,
Functional link between the mammalian exosome and mRNA decapping.
2001,
Pubmed Wilusz,
The cap-to-tail guide to mRNA turnover.
2001,
Pubmed
