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Fig. 4. Spatial expression of pax1 transcripts during Xenopus development. Whole-mount in situ hybridization was carried out using a digoxigenin-labeled riboprobe. A: Lateral view, ante- rior is to the right. A: At stage 22, pax1 is expressed in the branchial arches and weakly in the somite (red arrows). B: The pax1 expression is increased and its localization advances pro- gressively to caudal region of the somites in the stage 24 (B), stage 26 (C), and stage 35 embryos (D). E: Magnification of the dorsal region of D at the somite level. Black arrows indicate the intersomitic space. F: Whole-mount in situ hybridization with pax1 sense probe was used as negative control. G,H: Transverse sections of stage 35 embryos at the branchial arches level (G) and trunk (H). Nieuwenkoop-Faber stages are indicated on the lower left corner. BA, branchial arches; N, notochord; NT, neural tube; PE, pharyngeal endoderm; S, somite.
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pax1 (paired box 1) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 24, lateral view, anterior left, dorsal up.
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pax1 (paired box 1) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 26, lateral view, anterior left, dorsal up.
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pax1 (paired box 1) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 35/36, lateral view, anterior left, dorsal up.
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pax1 (paired box 1) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 35/36, lateral view, anterior left, dorsal up.
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Fig. 5. Spatial expression of pax9 transcripts during Xenopus development. Whole-mount pax9 in situ hybridization. A: Lateral view, anterior is to the right. A: At stage 22, pax9 is weakly expressed in the branchial arches and in the somite (red arrows). B: pax9 expression is increased and its localization advances progressively to caudal region in the stage 26 (B), stage 28 (C), and stage 35 embryos (D). E: Magnification of the dorsal region of C at the somites level. Black arrows indicate the intersomitic space. F: Whole-mount in situ hybridization with pax9 sense probe. G,H: Transverse sections of stage 35 embryos at the branchial arches level (G) and trunk (H).
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pax9 (paired box 9) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 26, lateral view, anterior left, dorsal up.
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pax9 (paired box 9) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 22, lateral view, anterior left, dorsal up.
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pax9 (paired box 9) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 28, lateral view, anterior left, dorsal up.
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pax9 (paired box 9) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 35/36, lateral view, anterior left, dorsal up.
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pax9 (paired box 9) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 35/36, lateral view, anterior left, dorsal up.
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Fig. 6. Spatial expression of uncx transcripts during Xenopus development. Whole-mount uncx in situ hybridization. A: Lateral view, anterior is to the right. A: At stage 21, uncx is weakly expressed in the branchial arches. B: At stage 26, uncx is strongly expressed in the branchial arches and in the anterior somitic region. C,D: Expression uncx in the in stage 28 (C) and stage 35 (D). E: Magnification of the dorsal region of C at the somites level. F: Whole-mount in situ hybridization with pax9 sense probe. G,H: Transverse sections of stage 35 embryos at the bran- chial arches level (G in panel D) and trunk (H in panel D).
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uncx (unc homeobox ) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 22, lateral view, anterior left, dorsal up.
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uncx (unc homeobox ) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 26, lateral view, anterior left, dorsal up.
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uncx (unc homeobox ) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 28, lateral view, anterior left, dorsal up.
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uncx (unc homeobox ) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 35, lateral view, anterior left, dorsal up.
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uncx (unc homeobox ) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 35, lateral view, anterior left, dorsal up.
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Transverse sections of stage 35 embryos at the branchial arches level. Displays pharyngeal endoderm (PE).
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