Yang X , Mao J , Walters EM , Zhao MT , Teson J , Lee K , Prather RS .

FIG. 1. Representative somatic cell nuclear transfer embryos at different stages of development reconstructed by using enhanced green fluorescent protein fibroblast cells. Only green embryos were counted and used for data analysis.

FIG. 2. Quantitative analysis of the DNA methylation status detected by 5MeC immunostaining in the control and Xenopus egg extract-treated cells (10D+extract: 10 μg/mL digitonin permeabilization of cells and Xenopus egg extract treatment; 15D+extract: 15 μg/mL digitonin permeabilization and Xenopus egg extract treatment) at days 0 (A) and 4 (B). The level of methylation is reported in arbitrary units and was detected using Texas Red-labeled antibodies (second) bound to primary antibodies specific to 5MeC. a, b, and c: Intensity of 5Mec staining in columns with different letters differ (a, b: p<0.05; a, c and b, c: p<0.01). 5MeC, 5-methylcytosine.

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