Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
J Biol Chem
2013 Aug 09;28832:23116-27. doi: 10.1074/jbc.M113.485516.
Show Gene links
Show Anatomy links
Sea anemone peptide with uncommon β-hairpin structure inhibits acid-sensing ion channel 3 (ASIC3) and reveals analgesic activity.
Osmakov DI
,
Kozlov SA
,
Andreev YA
,
Koshelev SG
,
Sanamyan NP
,
Sanamyan KE
,
Dyachenko IA
,
Bondarenko DA
,
Murashev AN
,
Mineev KS
,
Arseniev AS
,
Grishin EV
.
???displayArticle.abstract???
Three novel peptides were isolated from the venom of the sea anemone Urticina grebelnyi. All of them are 29 amino acid peptides cross-linked by two disulfide bridges, with a primary structure similar to other sea anemone peptides belonging to structural group 9a. The structure of the gene encoding the shared precursor protein of the identified peptides was determined. One peptide, π-AnmTX Ugr 9a-1 (short name Ugr 9-1), produced a reversible inhibition effect on both the transient and the sustained current of human ASIC3 channels expressed in Xenopus laevis oocytes. It completely blocked the transient component (IC50 10 ± 0.6 μM) and partially (48 ± 2%) inhibited the amplitude of the sustained component (IC50 1.44 ± 0.19 μM). Using in vivo tests in mice, Ugr 9-1 significantly reversed inflammatory and acid-induced pain. The other two novel peptides, AnmTX Ugr 9a-2 (Ugr 9-2) and AnmTX Ugr 9a-3 (Ugr 9-3), did not inhibit the ASIC3 current. NMR spectroscopy revealed that Ugr 9-1 has an uncommon spatial structure, stabilized by two S-S bridges, with three classical β-turns and twisted β-hairpin without interstrand disulfide bonds. This is a novel peptide spatial structure that we propose to name boundless β-hairpin.
Altschul,
Gapped BLAST and PSI-BLAST: a new generation of protein database search programs.
1997, Pubmed
Altschul,
Gapped BLAST and PSI-BLAST: a new generation of protein database search programs.
1997,
Pubmed
Anangi,
Functional expression in Escherichia coli of the disulfide-rich sea anemone peptide APETx2, a potent blocker of acid-sensing ion channel 3.
2012,
Pubmed
,
Xenbase
Andreev,
Molecules to selectively target receptors for treatment of pain and neurogenic inflammation.
2012,
Pubmed
Andreev,
Cyanogen bromide cleavage of proteins in salt and buffer solutions.
2010,
Pubmed
Andreev,
Analgesic compound from sea anemone Heteractis crispa is the first polypeptide inhibitor of vanilloid receptor 1 (TRPV1).
2008,
Pubmed
,
Xenbase
Andreev,
Analgesic effect of a polypeptide inhibitor of the TRPV1 receptor in noxious heat pain models.
2009,
Pubmed
Bosmans,
Sea anemone venom as a source of insecticidal peptides acting on voltage-gated Na+ channels.
2007,
Pubmed
Carli,
Toxicity of jellyfish and sea-anemone venoms on cultured V79 cells.
1996,
Pubmed
Castañeda,
Discovery and characterization of cnidarian peptide toxins that affect neuronal potassium ion channels.
2009,
Pubmed
Corzo,
Insecticidal peptides from the theraposid spider Brachypelma albiceps: an NMR-based model of Ba2.
2009,
Pubmed
,
Xenbase
Delaglio,
Measurement of homonuclear proton couplings from regular 2D COSY spectra.
2001,
Pubmed
Deval,
ASIC3, a sensor of acidic and primary inflammatory pain.
2008,
Pubmed
Deval,
Acid-sensing ion channels (ASICs): pharmacology and implication in pain.
2010,
Pubmed
Diochot,
A new sea anemone peptide, APETx2, inhibits ASIC3, a major acid-sensitive channel in sensory neurons.
2004,
Pubmed
,
Xenbase
Dubinnyi,
Lignan from thyme possesses inhibitory effect on ASIC3 channel current.
2012,
Pubmed
,
Xenbase
Escoubas,
Isolation of a tarantula toxin specific for a class of proton-gated Na+ channels.
2000,
Pubmed
,
Xenbase
Fahrner,
Solution structure of protegrin-1, a broad-spectrum antimicrobial peptide from porcine leukocytes.
1996,
Pubmed
Frishman,
Knowledge-based protein secondary structure assignment.
1995,
Pubmed
Grishin,
Novel peptide from spider venom inhibits P2X3 receptors and inflammatory pain.
2010,
Pubmed
Honma,
Novel peptide toxins from the sea anemone Stichodactyla haddoni.
2008,
Pubmed
Honma,
Isolation and molecular cloning of novel peptide toxins from the sea anemone Antheopsis maculata.
2005,
Pubmed
Hwang,
Three-dimensional solution structure of lactoferricin B, an antimicrobial peptide derived from bovine lactoferrin.
1998,
Pubmed
Karczewski,
Reversal of acid-induced and inflammatory pain by the selective ASIC3 inhibitor, APETx2.
2010,
Pubmed
Kellenberger,
Epithelial sodium channel/degenerin family of ion channels: a variety of functions for a shared structure.
2002,
Pubmed
Kozlov,
[Polypeptide toxin from sea anemone inhibiting proton-sensitive channel ASIC3].
2012,
Pubmed
,
Xenbase
Kozlov,
The universal algorithm of maturation for secretory and excretory protein precursors.
2007,
Pubmed
Kozlov,
Latarcins, antimicrobial and cytolytic peptides from the venom of the spider Lachesana tarabaevi (Zodariidae) that exemplify biomolecular diversity.
2006,
Pubmed
Kozlov,
A novel strategy for the identification of toxinlike structures in spider venom.
2005,
Pubmed
Kozlov,
The mining of toxin-like polypeptides from EST database by single residue distribution analysis.
2011,
Pubmed
Kozlov,
Classification of spider neurotoxins using structural motifs by primary structure features. Single residue distribution analysis and pattern analysis techniques.
2005,
Pubmed
Kozlov,
Convenient nomenclature of cysteine-rich polypeptide toxins from sea anemones.
2012,
Pubmed
Lai,
Antimicrobial peptides from skin secretions of Chinese red belly toad Bombina maxima.
2002,
Pubmed
Lovelace,
Cyclic MrIA: a stable and potent cyclic conotoxin with a novel topological fold that targets the norepinephrine transporter.
2006,
Pubmed
Marino,
Cytotoxicity of the nematocyst venom from the sea anemone Aiptasia mutabilis.
2004,
Pubmed
Molliver,
ASIC3, an acid-sensing ion channel, is expressed in metaboreceptive sensory neurons.
2005,
Pubmed
Salinas,
Structural elements for the generation of sustained currents by the acid pain sensor ASIC3.
2009,
Pubmed
,
Xenbase
Shenkarev,
Molecular mechanism of action of β-hairpin antimicrobial peptide arenicin: oligomeric structure in dodecylphosphocholine micelles and pore formation in planar lipid bilayers.
2011,
Pubmed
Shiomi,
Novel peptide toxins recently isolated from sea anemones.
2009,
Pubmed
Shlyapnikov,
Bacterial production of latarcin 2a, a potent antimicrobial peptide from spider venom.
2008,
Pubmed
Terry,
The cDNA sequence coding for prepro-PGS (prepro-magainins) and aspects of the processing of this prepro-polypeptide.
1988,
Pubmed
,
Xenbase
Umetsu,
C-terminal elongation of growth-blocking peptide enhances its biological activity and micelle binding affinity.
2009,
Pubmed
Vassilevski,
Antimicrobial peptide precursor structures suggest effective production strategies.
2008,
Pubmed
Vetter,
Venomics: a new paradigm for natural products-based drug discovery.
2011,
Pubmed
Voilley,
Nonsteroid anti-inflammatory drugs inhibit both the activity and the inflammation-induced expression of acid-sensing ion channels in nociceptors.
2001,
Pubmed
Yen,
Role of acid-sensing ion channel 3 in sub-acute-phase inflammation.
2009,
Pubmed
Zaharenko,
Proteomics of the neurotoxic fraction from the sea anemone Bunodosoma cangicum venom: Novel peptides belonging to new classes of toxins.
2008,
Pubmed
