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Cell Cycle
2014 Jan 01;139:1482-8. doi: 10.4161/cc.28432.
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Specificity of calcium/calmodulin-dependent protein kinases in mouse egg activation.
Medvedev S
,
Stein P
,
Schultz RM
.
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CaMKIIγ, the predominant CaMKII isoform in mouse eggs, controls egg activation by regulating cell cycle resumption. In this study we further characterize the involvement and specificity of CaMKIIγ in mouse egg activation. Using exogenous expression of different cRNAs in Camk2g(-/-) eggs, we show that the other multifunctional CaM kinases, CaMKI, and CaMKIV, are not capable of substituting CaMKIIγ to initiate cell cycle resumption in response to a rise in intracellular Ca (2+). Exogenous expression of Camk2g or Camk2d results in activation of nearly 80% of Camk2g(-/-) MII eggs after stimulation with SrCl 2, which does not differ from the incidence of activation of wild-type eggs expressing exogenous Egfp. In contrast, none of the Camk2g(-/-) MII eggs expressing Camk1 or Camk4 activate in response to SrCl 2 treatment. Expression of a constitutively active form of Camk4 (ca-Camk4), but not Camk1, triggers egg activation. EMI2, an APC/C repressor, is a key component in regulating egg activation downstream of CaMKII in both Xenopus laevis and mouse. We show that exogenous expression of either Camk2g, Camk2d, or ca-Camk4, but not Camk1, Camk4, or a catalytically inactive mutant form of CaMKIIγ (kinase-dead) in Camk2g(-/-) mouse eggs leads to almost complete degradation (~90%) of exogenously expressed EMI2 followed by cell cycle resumption. Thus, degradation of EMI2 following its phosphorylation specifically by CaMKII is mechanistically linked to and promotes cell cycle resumption in MII eggs.
Backs,
The gamma isoform of CaM kinase II controls mouse egg activation by regulating cell cycle resumption.
2010, Pubmed
Backs,
The gamma isoform of CaM kinase II controls mouse egg activation by regulating cell cycle resumption.
2010,
Pubmed
Bayer,
Developmental expression of the CaM kinase II isoforms: ubiquitous gamma- and delta-CaM kinase II are the early isoforms and most abundant in the developing nervous system.
1999,
Pubmed
Berridge,
Calcium--a life and death signal.
1998,
Pubmed
Chang,
Calmodulin-dependent protein kinase gamma 3 (CamKIIgamma3) mediates the cell cycle resumption of metaphase II eggs in mouse.
2009,
Pubmed
Chatot,
An improved culture medium supports development of random-bred 1-cell mouse embryos in vitro.
1989,
Pubmed
Ducibella,
The roles of Ca2+, downstream protein kinases, and oscillatory signaling in regulating fertilization and the activation of development.
2008,
Pubmed
Ducibella,
Egg-to-embryo transition is driven by differential responses to Ca(2+) oscillation number.
2002,
Pubmed
Gaertner,
Comparative analyses of the three-dimensional structures and enzymatic properties of alpha, beta, gamma and delta isoforms of Ca2+-calmodulin-dependent protein kinase II.
2004,
Pubmed
Hanson,
Dual role of calmodulin in autophosphorylation of multifunctional CaM kinase may underlie decoding of calcium signals.
1994,
Pubmed
Ho,
Preimplantation development of mouse embryos in KSOM: augmentation by amino acids and analysis of gene expression.
1995,
Pubmed
Hook,
Ca(2+)/CaM-dependent kinases: from activation to function.
2001,
Pubmed
Igarashi,
Alterations of PLCbeta1 in mouse eggs change calcium oscillatory behavior following fertilization.
2007,
Pubmed
Knott,
Calmodulin-dependent protein kinase II triggers mouse egg activation and embryo development in the absence of Ca2+ oscillations.
2006,
Pubmed
Madgwick,
Calmodulin-dependent protein kinase II, and not protein kinase C, is sufficient for triggering cell-cycle resumption in mammalian eggs.
2005,
Pubmed
Madgwick,
Mouse Emi2 is required to enter meiosis II by reestablishing cyclin B1 during interkinesis.
2006,
Pubmed
Markoulaki,
Oscillatory CaMKII activity in mouse egg activation.
2003,
Pubmed
Perry,
Second meiotic arrest and exit in frogs and mice.
2008,
Pubmed
,
Xenbase
Schmidt,
Cytostatic factor: an activity that puts the cell cycle on hold.
2006,
Pubmed
,
Xenbase
Schultz,
Molecular basis of mammalian egg activation.
1995,
Pubmed
Shoji,
Mammalian Emi2 mediates cytostatic arrest and transduces the signal for meiotic exit via Cdc20.
2006,
Pubmed
,
Xenbase
Skelding,
Controlling the cell cycle: the role of calcium/calmodulin-stimulated protein kinases I and II.
2011,
Pubmed
Suzuki,
Full-term mouse development by abolishing Zn2+-dependent metaphase II arrest without Ca2+ release.
2010,
Pubmed
Suzuki,
Mouse Emi2 as a distinctive regulatory hub in second meiotic metaphase.
2010,
Pubmed
,
Xenbase
Swann,
PLCzeta(zeta): a sperm protein that triggers Ca2+ oscillations and egg activation in mammals.
2006,
Pubmed
Takaishi,
Evidence for distinct neuronal localization of gamma and delta subunits of Ca2+/calmodulin-dependent protein kinase II in the rat brain.
1992,
Pubmed
Tokumitsu,
Activation mechanisms for Ca2+/calmodulin-dependent protein kinase IV. Identification of a brain CaM-kinase IV kinase.
1994,
Pubmed
Wayman,
Analysis of CaM-kinase signaling in cells.
2011,
Pubmed
Wayman,
Regulation of axonal extension and growth cone motility by calmodulin-dependent protein kinase I.
2004,
Pubmed
