Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
J Biomed Opt
2014 Aug 01;198:086005. doi: 10.1117/1.JBO.19.8.086005.
Show Gene links
Show Anatomy links
Label-free real-time imaging of myelination in the Xenopus laevis tadpole by in vivo stimulated Raman scattering microscopy.
Hu CR
,
Zhang D
,
Slipchenko MN
,
Cheng JX
,
Hu B
.
???displayArticle.abstract???
The myelin sheath plays an important role as the axon in the functioning of the neural system, and myelin degradation is a hallmark pathology of multiple sclerosis and spinal cord injury. Electron microscopy, fluorescent microscopy, and magnetic resonance imaging are three major techniques used for myelin visualization. However, microscopic observation of myelin in living organisms remains a challenge. Using a newly developed stimulated Raman scattering microscopy approach, we report noninvasive, label-free, real-time in vivo imaging of myelination by a single-Schwann cell, maturation of a single node of Ranvier, and myelin degradation in the transparent body of the Xenopus laevis tadpole.
Aggarwal,
Central nervous system myelin: structure, synthesis and assembly.
2011, Pubmed
Aggarwal,
Central nervous system myelin: structure, synthesis and assembly.
2011,
Pubmed
Alsina,
Visualizing synapse formation in arborizing optic axons in vivo: dynamics and modulation by BDNF.
2001,
Pubmed
,
Xenbase
Anitei,
Myelin biogenesis: sorting out protein trafficking.
2006,
Pubmed
Bakshi,
MRI in multiple sclerosis: current status and future prospects.
2008,
Pubmed
Bélanger,
In vivo evaluation of demyelination and remyelination in a nerve crush injury model.
2011,
Pubmed
Bentley,
Real-time image guidance for brain tumor surgery through stimulated Raman scattering microscopy.
2014,
Pubmed
Bible,
Multiple sclerosis: Drug-enhanced remyelination in a multiple sclerosis model.
2013,
Pubmed
Ceccarelli,
MRI in multiple sclerosis: a review of the current literature.
2012,
Pubmed
Cohen-Cory,
Imaging retinotectal synaptic connectivity.
2007,
Pubmed
,
Xenbase
Confavreux,
[Central nervous system demyelinating diseases: an overview].
2010,
Pubmed
Crane,
Using Xenopus oocyte extracts to study signal transduction.
2006,
Pubmed
,
Xenbase
Czopka,
Individual oligodendrocytes have only a few hours in which to generate new myelin sheaths in vivo.
2013,
Pubmed
Dawid,
Xenopus laevis in developmental and molecular biology.
1988,
Pubmed
,
Xenbase
Dong,
A competition-based mechanism mediates developmental refinement of tectal neuron receptive fields.
2012,
Pubmed
,
Xenbase
Dubois-Dalcq,
From fish to man: understanding endogenous remyelination in central nervous system demyelinating diseases.
2008,
Pubmed
,
Xenbase
Eshed-Eisenbach,
The making of a node: a co-production of neurons and glia.
2013,
Pubmed
Evans,
Coherent anti-stokes Raman scattering microscopy: chemical imaging for biology and medicine.
2008,
Pubmed
Fancy,
Myelin regeneration: a recapitulation of development?
2011,
Pubmed
Farrar,
Chronic in vivo imaging in the mouse spinal cord using an implanted chamber.
2012,
Pubmed
FERNANDEZ-MORAN,
[Electron microscope examination of the myelin sheath and axial cylinder in the internodal section of neural fibers].
1950,
Pubmed
Franklin,
Remyelination in the CNS: from biology to therapy.
2008,
Pubmed
Franklin,
Why does remyelination fail in multiple sclerosis?
2002,
Pubmed
Freudiger,
Label-free biomedical imaging with high sensitivity by stimulated Raman scattering microscopy.
2008,
Pubmed
Fu,
Coherent anti-Stokes Raman scattering imaging of myelin degradation reveals a calcium-dependent pathway in lyso-PtdCho-induced demyelination.
2007,
Pubmed
Fu,
Paranodal myelin retraction in relapsing experimental autoimmune encephalomyelitis visualized by coherent anti-Stokes Raman scattering microscopy.
2011,
Pubmed
Hartline,
Rapid conduction and the evolution of giant axons and myelinated fibers.
2007,
Pubmed
Hirano,
A structural analysis of the myelin sheath in the central nervous system.
1967,
Pubmed
Hu,
Stimulated Raman scattering imaging by continuous-wave laser excitation.
2013,
Pubmed
Hu,
BDNF stabilizes synapses and maintains the structural complexity of optic axons in vivo.
2005,
Pubmed
,
Xenbase
Huff,
Real-time CARS imaging reveals a calpain-dependent pathway for paranodal myelin retraction during high-frequency stimulation.
2011,
Pubmed
Imitola,
Multimodal coherent anti-Stokes Raman scattering microscopy reveals microglia-associated myelin and axonal dysfunction in multiple sclerosis-like lesions in mice.
2011,
Pubmed
Ji,
Rapid, label-free detection of brain tumors with stimulated Raman scattering microscopy.
2013,
Pubmed
Kälin,
An in vivo chemical library screen in Xenopus tadpoles reveals novel pathways involved in angiogenesis and lymphangiogenesis.
2009,
Pubmed
,
Xenbase
Kaya,
Live imaging of targeted cell ablation in Xenopus: a new model to study demyelination and repair.
2012,
Pubmed
,
Xenbase
Kazarinova-Noyes,
Molecular constituents of the node of Ranvier.
2002,
Pubmed
Kirby,
In vivo time-lapse imaging shows dynamic oligodendrocyte progenitor behavior during zebrafish development.
2006,
Pubmed
Kvist,
The use of Xenopus oocytes in drug screening.
2011,
Pubmed
,
Xenbase
Miller,
Dissecting demyelination.
2007,
Pubmed
Min,
Coherent nonlinear optical imaging: beyond fluorescence microscopy.
2011,
Pubmed
Peles,
Molecular domains of myelinated axons.
2000,
Pubmed
Poliak,
The local differentiation of myelinated axons at nodes of Ranvier.
2003,
Pubmed
Sherman,
Mechanisms of axon ensheathment and myelin growth.
2005,
Pubmed
Shi,
Longitudinal in vivo coherent anti-Stokes Raman scattering imaging of demyelination and remyelination in injured spinal cord.
2011,
Pubmed
Simons,
Wrapping it up: the cell biology of myelination.
2007,
Pubmed
Simons,
Assembly of myelin by association of proteolipid protein with cholesterol- and galactosylceramide-rich membrane domains.
2000,
Pubmed
Slipchenko,
Heterodyne detected nonlinear optical imaging in a lock-in free manner.
2012,
Pubmed
Snaidero,
Myelin membrane wrapping of CNS axons by PI(3,4,5)P3-dependent polarized growth at the inner tongue.
2014,
Pubmed
Susuki,
Molecular mechanisms of node of Ranvier formation.
2008,
Pubmed
Takagi,
Transgenic Xenopus laevis for live imaging in cell and developmental biology.
2013,
Pubmed
,
Xenbase
Thaxton,
Nodes of Ranvier act as barriers to restrict invasion of flanking paranodal domains in myelinated axons.
2011,
Pubmed
Tomlinson,
Chemical genetics and drug discovery in Xenopus.
2012,
Pubmed
,
Xenbase
van der Goes,
Models for demyelination.
2001,
Pubmed
Viczian,
Generation of functional eyes from pluripotent cells.
2009,
Pubmed
,
Xenbase
Wang,
Label-free quantitative imaging of cholesterol in intact tissues by hyperspectral stimulated Raman scattering microscopy.
2013,
Pubmed
Wang,
Coherent anti-stokes Raman scattering imaging of axonal myelin in live spinal tissues.
2005,
Pubmed
Wei,
Vibrational imaging of newly synthesized proteins in live cells by stimulated Raman scattering microscopy.
2013,
Pubmed
Yin,
Knockdown of Lingo1b protein promotes myelination and oligodendrocyte differentiation in zebrafish.
2014,
Pubmed
Zhang,
Highly Sensitive Vibrational Imaging by Femtosecond Pulse Stimulated Raman Loss.
2011,
Pubmed
Zhang,
Assembly and maintenance of nodes of ranvier rely on distinct sources of proteins and targeting mechanisms.
2012,
Pubmed
