Daeffler KN , Lester HA , Dougherty DA .

NS034407 NINDS NIH HHS , R01 NS034407 NINDS NIH HHS , R37 NS034407 NINDS NIH HHS

	Figure 1. Glutamate binding site and predicted electrostatic interactions between the ligand and its surrounding amino acids. Residues from the primary face are colored in green and the complementary face in blue.
	Figure 2. Chemical structures of amino acids used in this study.
	Figure 3. Plot of cation−π binding energy vs mutant receptor loss-of-function at Y206. A linear trend is indicative of an electrostatic interaction with the face of the residue examined. Data for both the wild type and α signal peptide-containing templates are plotted together and indicate no effect of altering the signal peptide on receptor function.
	Figure 4. Predicted hydrogen bond interactions between the primary and complementary subunits near the ligand-binding site in the 3RIF crystal structure. Residues on the primary face are shown in green, residues on the complementary face in blue, and glutamate in white. Hydrogen bond network between (A) the C loop of the primary subunit and the F loop of the complementary subunit and (B) the B loop of the primary subunit and the E loop of the complementary subunit.

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