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XB-ART-49473
Science 2014 Oct 10;3466206:244-7. doi: 10.1126/science.1256773.
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Spatial organization of cytokinesis signaling reconstituted in a cell-free system.

Nguyen PA , Groen AC , Loose M , Ishihara K , Wühr M , Field CM , Mitchison TJ .


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During animal cell division, the cleavage furrow is positioned by microtubules that signal to the actin cortex at the cell midplane. We developed a cell-free system to recapitulate cytokinesis signaling using cytoplasmic extract from Xenopus eggs. Microtubules grew out as asters from artificial centrosomes and met to organize antiparallel overlap zones. These zones blocked the interpenetration of neighboring asters and recruited cytokinesis midzone proteins, including the chromosomal passenger complex (CPC) and centralspindlin. The CPC was transported to overlap zones, which required two motor proteins, Kif4A and a Kif20A paralog. Using supported lipid bilayers to mimic the plasma membrane, we observed the recruitment of cleavage furrow markers, including an active RhoA reporter, at microtubule overlaps. This system opens further approaches to understanding the biophysics of cytokinesis signaling.

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Species referenced: Xenopus laevis
Genes referenced: actl6a kif11 kif20a kif23 kif4a rhoa

References [+] :
Applegate, plusTipTracker: Quantitative image analysis software for the measurement of microtubule dynamics. 2011, Pubmed