Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Controlled surface topography regulates collective 3D migration by epithelial-mesenchymal composite embryonic tissues.
Song J
,
Shawky JH
,
Kim Y
,
Hazar M
,
LeDuc PR
,
Sitti M
.
???displayArticle.abstract???
Cells in tissues encounter a range of physical cues as they migrate. Probing single cell and collective migratory responses to physically defined three-dimensional (3D) microenvironments and the factors that modulate those responses are critical to understanding how tissue migration is regulated during development, regeneration, and cancer. One key physical factor that regulates cell migration is topography. Most studies on surface topography and cell mechanics have been carried out with single migratory cells, yet little is known about the spreading and motility response of 3D complex multi-cellular tissues to topographical cues. Here, we examine the response to complex topographical cues of microsurgically isolated tissue explants composed of epithelial and mesenchymal cell layers from naturally 3D organized embryos of the aquatic frog Xenopus laevis. We control topography using fabricated micropost arrays (MPAs) and investigate the collective 3D migration of these multi-cellular systems in these MPAs. We find that the topography regulates both collective and individual cell migration and that dense MPAs reduce but do not eliminate tissue spreading. By modulating cell size through the cell cycle inhibitor Mitomycin C or the spacing of the MPAs we uncover how 3D topographical cues disrupt collective cell migration. We find surface topography can direct both single cell motility and tissue spreading, altering tissue-scale processes that enable efficient conversion of single cell motility into collective movement.
Abercrombie,
The locomotion of fibroblasts in culture. IV. Electron microscopy of the leading lamella.
1971, Pubmed
Abercrombie,
The locomotion of fibroblasts in culture. IV. Electron microscopy of the leading lamella.
1971,
Pubmed
Abercrombie,
Adhesions of fibroblasts to substratum during contact inhibition observed by interference reflection microscopy.
1975,
Pubmed
ABERCROMBIE,
Observations on the social behaviour of cells in tissue culture. I. Speed of movement of chick heart fibroblasts in relation to their mutual contacts.
1953,
Pubmed
Bravo-Cordero,
Directed cell invasion and migration during metastasis.
2012,
Pubmed
Burton,
Keratocytes generate traction forces in two phases.
1999,
Pubmed
Burton,
Traction forces of cytokinesis measured with optically modified elastic substrata.
1997,
Pubmed
Chen,
Micropatterned surfaces for control of cell shape, position, and function.
1998,
Pubmed
Cooke,
Properties of the primary organization field in the embryo of Xenopus laevis. IV. Pattern formation and regulation following early inhibition of mitosis.
1973,
Pubmed
,
Xenbase
Couchman,
Actomyosin organisation for adhesion, spreading, growth and movement in chick fibroblasts.
1979,
Pubmed
CURTIS,
CONTROL OF CELL BEHAVIOR: TOPOLOGICAL FACTORS.
1964,
Pubmed
Danilchick,
Xenopus laevis: Practical uses in cell and molecular biology. Pictorial collage of embryonic stages.
1991,
Pubmed
,
Xenbase
Dembo,
Stresses at the cell-to-substrate interface during locomotion of fibroblasts.
1999,
Pubmed
du Roure,
Force mapping in epithelial cell migration.
2005,
Pubmed
Edelstein,
Computer control of microscopes using µManager.
2010,
Pubmed
Engler,
Matrix elasticity directs stem cell lineage specification.
2006,
Pubmed
Folkman,
Role of cell shape in growth control.
1978,
Pubmed
Galbraith,
A micromachined device provides a new bend on fibroblast traction forces.
1997,
Pubmed
Ghibaudo,
Mechanics of cell spreading within 3D-micropatterned environments.
2011,
Pubmed
Gong,
Planar cell polarity signalling controls cell division orientation during zebrafish gastrulation.
2004,
Pubmed
Guo,
Substrate rigidity regulates the formation and maintenance of tissues.
2006,
Pubmed
Gurtner,
Wound repair and regeneration.
2008,
Pubmed
Harris,
Silicone rubber substrata: a new wrinkle in the study of cell locomotion.
1980,
Pubmed
Hoffman-Kim,
Topography, cell response, and nerve regeneration.
2010,
Pubmed
Khademhosseini,
Microscale technologies for tissue engineering and biology.
2006,
Pubmed
Kim,
Mechanochemical actuators of embryonic epithelial contractility.
2014,
Pubmed
,
Xenbase
Lange,
Cell and tissue mechanics in cell migration.
2013,
Pubmed
Lee,
Traction forces generated by locomoting keratocytes.
1994,
Pubmed
Liu,
Mechanical tugging force regulates the size of cell-cell junctions.
2010,
Pubmed
Lo,
Cell movement is guided by the rigidity of the substrate.
2000,
Pubmed
Martin,
Wound healing--aiming for perfect skin regeneration.
1997,
Pubmed
Mayor,
Keeping in touch with contact inhibition of locomotion.
2010,
Pubmed
Mohr,
3-D microwell culture of human embryonic stem cells.
2006,
Pubmed
Pelham,
Cell locomotion and focal adhesions are regulated by substrate flexibility.
1997,
Pubmed
Petrie,
Random versus directionally persistent cell migration.
2009,
Pubmed
Poujade,
Collective migration of an epithelial monolayer in response to a model wound.
2007,
Pubmed
Rossier,
Force generated by actomyosin contraction builds bridges between adhesive contacts.
2010,
Pubmed
Saez,
Rigidity-driven growth and migration of epithelial cells on microstructured anisotropic substrates.
2007,
Pubmed
Sheetz,
Cell migration: regulation of force on extracellular-matrix-integrin complexes.
1998,
Pubmed
Singer,
Cutaneous wound healing.
1999,
Pubmed
Sniadecki,
Magnetic microposts as an approach to apply forces to living cells.
2007,
Pubmed
Tambe,
Collective cell guidance by cooperative intercellular forces.
2011,
Pubmed
Tan,
Cells lying on a bed of microneedles: an approach to isolate mechanical force.
2003,
Pubmed
Teixeira,
Epithelial contact guidance on well-defined micro- and nanostructured substrates.
2003,
Pubmed
Theveneau,
Chase-and-run between adjacent cell populations promotes directional collective migration.
2013,
Pubmed
Tseng,
A new micropatterning method of soft substrates reveals that different tumorigenic signals can promote or reduce cell contraction levels.
2011,
Pubmed
von Dassow,
Surprisingly simple mechanical behavior of a complex embryonic tissue.
2010,
Pubmed
,
Xenbase
Yamada,
Fibronectins--adhesive glycoproteins of cell surface and blood.
1978,
Pubmed
Yamaguchi,
Cell migration in tumors.
2005,
Pubmed
Yang,
Assaying stem cell mechanobiology on microfabricated elastomeric substrates with geometrically modulated rigidity.
2011,
Pubmed
Yeung,
Effects of substrate stiffness on cell morphology, cytoskeletal structure, and adhesion.
2005,
Pubmed
