Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Mek2 is dispensable for mouse growth and development.
Bélanger LF
,
Roy S
,
Tremblay M
,
Brott B
,
Steff AM
,
Mourad W
,
Hugo P
,
Erikson R
,
Charron J
.
???displayArticle.abstract???
MEK is a dual-specificity kinase that activates the extracellular signal-regulated kinase (ERK) mitogen-activated protein (MAP) kinase upon agonist binding to receptors. The ERK/MAP kinase cascade is involved in cell fate determination in many organisms. In mammals, this pathway is proposed to regulate cell growth and differentiation. Genetic studies have shown that although a single Mek gene is present in Caenorhabditis elegans, Drosophila melanogaster, and Xenopus laevis, two Mek homologs, Mek1 and Mek2, are present in the mammalian cascade. The inactivation of the Mek1 gene leads to embryonic lethality and has revealed the unique role played by Mek1 during embryogenesis. To investigate the biological function of the second homolog, we have generated mice deficient in Mek2 function. Mek2 mutant mice are viable and fertile, and they do not present flagrant morphological alteration. Although several components of the ERK/MAP kinase cascade have been implicated in thymocyte development, no such involvement was observed for MEK2, which appears to be nonessential for thymocyte differentiation and T-cell-receptor-induced proliferation and apoptosis. Altogether, our findings demonstrate that MEK2 is not necessary for the normal development of the embryo and T-cell lineages, suggesting that the loss of MEK2 can be compensated for by MEK1.
Acharya,
Signaling via mitogen-activated protein kinase kinase (MEK1) is required for Golgi fragmentation during mitosis.
1998, Pubmed
Acharya,
Signaling via mitogen-activated protein kinase kinase (MEK1) is required for Golgi fragmentation during mitosis.
1998,
Pubmed
Alberola-Ila,
Selective requirement for MAP kinase activation in thymocyte differentiation.
1995,
Pubmed
Alberola-Ila,
Positive and negative selection invoke distinct signaling pathways.
1996,
Pubmed
Aubry,
N-Myc shares cellular functions wiht c-Myc.
2000,
Pubmed
Bhunia,
Lactosylceramide stimulates Ras-GTP loading, kinases (MEK, Raf), p44 mitogen-activated protein kinase, and c-fos expression in human aortic smooth muscle cells.
1996,
Pubmed
Bommhardt,
MEK activity regulates negative selection of immature CD4+CD8+ thymocytes.
2000,
Pubmed
Brott,
MEK2 is a kinase related to MEK1 and is differentially expressed in murine tissues.
1993,
Pubmed
Catling,
A proline-rich sequence unique to MEK1 and MEK2 is required for raf binding and regulates MEK function.
1995,
Pubmed
Charron,
High-frequency disruption of the N-myc gene in embryonic stem and pre-B cell lines by homologous recombination.
1990,
Pubmed
Charron,
Embryonic lethality in mice homozygous for a targeted disruption of the N-myc gene.
1992,
Pubmed
Chen,
Integrin-mediated cell adhesion activates mitogen-activated protein kinases.
1994,
Pubmed
Cobb,
MAP kinase pathways.
1999,
Pubmed
Crews,
The primary structure of MEK, a protein kinase that phosphorylates the ERK gene product.
1992,
Pubmed
Crompton,
The MAP kinase pathway controls differentiation from double-negative to double-positive thymocyte.
1996,
Pubmed
Dang,
The MEK1 proline-rich insert is required for efficient activation of the mitogen-activated protein kinases ERK1 and ERK2 in mammalian cells.
1998,
Pubmed
Eblen,
Rac-PAK signaling stimulates extracellular signal-regulated kinase (ERK) activation by regulating formation of MEK1-ERK complexes.
2002,
Pubmed
Fukuda,
Interaction of MAP kinase with MAP kinase kinase: its possible role in the control of nucleocytoplasmic transport of MAP kinase.
1997,
Pubmed
Giroux,
Embryonic death of Mek1-deficient mice reveals a role for this kinase in angiogenesis in the labyrinthine region of the placenta.
1999,
Pubmed
,
Xenbase
Guo,
Disruption of Mekk2 in mice reveals an unexpected role for MEKK2 in modulating T-cell receptor signal transduction.
2002,
Pubmed
Hanks,
The protein kinase family: conserved features and deduced phylogeny of the catalytic domains.
1988,
Pubmed
Hsu,
A temperature-sensitive MEK mutation demonstrates the conservation of the signaling pathways activated by receptor tyrosine kinases.
1994,
Pubmed
Ioffe,
WW6: an embryonic stem cell line with an inert genetic marker that can be traced in chimeras.
1995,
Pubmed
Janeway,
T-cell receptors: is the repertoire inherently MHC-specific?
1997,
Pubmed
Jelinek,
RAS and RAF-1 form a signalling complex with MEK-1 but not MEK-2.
1994,
Pubmed
Johnson,
Sequential protein kinase reactions controlling cell growth and differentiation.
1994,
Pubmed
Killeen,
Signaling checkpoints during the development of T lymphocytes.
1998,
Pubmed
Klemke,
Regulation of cell motility by mitogen-activated protein kinase.
1997,
Pubmed
Kornfeld,
The Caenorhabditis elegans gene mek-2 is required for vulval induction and encodes a protein similar to the protein kinase MEK.
1995,
Pubmed
Malynn,
Generation of normal lymphocytes derived from N-myc-deficient embryonic stem cells.
1995,
Pubmed
Mansour,
Disruption of the proto-oncogene int-2 in mouse embryo-derived stem cells: a general strategy for targeting mutations to non-selectable genes.
1988,
Pubmed
Nantel,
Interaction of the Grb10 adapter protein with the Raf1 and MEK1 kinases.
1998,
Pubmed
Pagès,
Constitutive mutant and putative regulatory serine phosphorylation site of mammalian MAP kinase kinase (MEK1).
1994,
Pubmed
Pagès,
Defective thymocyte maturation in p44 MAP kinase (Erk 1) knockout mice.
1999,
Pubmed
Papin,
Identification of signalling proteins interacting with B-Raf in the yeast two-hybrid system.
1996,
Pubmed
Rodewald,
Molecular and cellular events in early thymocyte development.
1998,
Pubmed
Rousseau,
p38 MAP kinase activation by vascular endothelial growth factor mediates actin reorganization and cell migration in human endothelial cells.
1997,
Pubmed
Russell,
Regulation of recombinant MEK1 and MEK2b expressed in Escherichia coli.
1995,
Pubmed
Seger,
The MAPK signaling cascade.
1995,
Pubmed
Sétáló,
Estradiol-induced phosphorylation of ERK1/2 in explants of the mouse cerebral cortex: the roles of heat shock protein 90 (Hsp90) and MEK2.
2002,
Pubmed
Seufferlein,
Reduced requirement of mitogen-activated protein kinase (MAPK) activity for entry into the S phase of the cell cycle in Swiss 3T3 fibroblasts stimulated by bombesin and insulin.
1996,
Pubmed
Umbhauer,
Mesoderm induction in Xenopus caused by activation of MAP kinase.
1995,
Pubmed
,
Xenbase
Winston,
Preferential involvement of MEK1 in the tumor necrosis factor-alpha-induced activation of p42mapk/erk2 in mouse macrophages.
1995,
Pubmed
Wu,
MEK-2, a Caenorhabditis elegans MAP kinase kinase, functions in Ras-mediated vulval induction and other developmental events.
1995,
Pubmed
Wu,
Selective activation of MEK1 but not MEK2 by A-Raf from epidermal growth factor-stimulated Hela cells.
1996,
Pubmed
Zheng,
Properties of MEKs, the kinases that phosphorylate and activate the extracellular signal-regulated kinases.
1993,
Pubmed
