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Cell division in prokaryotes and eukaryotes is commonly initiated by the well-controlled binding of proteins to the cytoplasmic side of the cell membrane. However, a precise characterization of the spatiotemporal dynamics of membrane-bound proteins is often difficult to achieve in vivo. Here, we present protocols for the use of supported lipid bilayers to rebuild the cytokinetic machineries of cells with greatly different dimensions: the bacterium Escherichia coli and eggs of the vertebrate Xenopus laevis. Combined with total internal reflection fluorescence microscopy, these experimental setups allow for precise quantitative analyses of membrane-bound proteins. The protocols described to obtain glass-supported membranes from bacterial and vertebrate lipids can be used as starting points for other reconstitution experiments. We believe that similar biochemical assays will be instrumental to study the biochemistry and biophysics underlying a variety of complex cellular tasks, such as signaling, vesicle trafficking, and cell motility.
Abel,
The membrane environment can promote or suppress bistability in cell signaling networks.
2012, Pubmed
Abel,
The membrane environment can promote or suppress bistability in cell signaling networks.
2012,
Pubmed
Brian,
Allogeneic stimulation of cytotoxic T cells by supported planar membranes.
1984,
Pubmed
Bridges,
Septin assemblies form by diffusion-driven annealing on membranes.
2014,
Pubmed
Castellana,
Solid supported lipid bilayers: From biophysical studies to sensor design.
2006,
Pubmed
Chen,
Orchestrating vesicle transport, ESCRTs and kinase surveillance during abscission.
2012,
Pubmed
Desai,
The use of Xenopus egg extracts to study mitotic spindle assembly and function in vitro.
1999,
Pubmed
,
Xenbase
Di Paolo,
Phosphoinositides in cell regulation and membrane dynamics.
2006,
Pubmed
Elf,
Spontaneous separation of bi-stable biochemical systems into spatial domains of opposite phases.
2004,
Pubmed
Field,
Actin behavior in bulk cytoplasm is cell cycle regulated in early vertebrate embryos.
2011,
Pubmed
,
Xenbase
Field,
Xenopus egg cytoplasm with intact actin.
2014,
Pubmed
,
Xenbase
Galush,
Quantitative fluorescence microscopy using supported lipid bilayer standards.
2008,
Pubmed
Groen,
Glycogen-supplemented mitotic cytosol for analyzing Xenopus egg microtubule organization.
2014,
Pubmed
,
Xenbase
Groves,
Micropatterning fluid lipid bilayers on solid supports.
1997,
Pubmed
Groves,
Molecular mechanisms in signal transduction at the membrane.
2010,
Pubmed
Hyman,
Preparation of modified tubulins.
1991,
Pubmed
Jung,
Multivalent ligand-receptor binding on supported lipid bilayers.
2009,
Pubmed
Kholodenko,
Why cytoplasmic signalling proteins should be recruited to cell membranes.
2000,
Pubmed
Kochanczyk,
Stochastic transitions in a bistable reaction system on the membrane.
2013,
Pubmed
Lee,
Self-assembly of filopodia-like structures on supported lipid bilayers.
2010,
Pubmed
,
Xenbase
Leonard,
Regulation of protein kinases by lipids.
2011,
Pubmed
Lin,
H-Ras forms dimers on membrane surfaces via a protein-protein interface.
2014,
Pubmed
Loose,
Spatial regulators for bacterial cell division self-organize into surface waves in vitro.
2008,
Pubmed
Loose,
The bacterial cell division proteins FtsA and FtsZ self-organize into dynamic cytoskeletal patterns.
2014,
Pubmed
Murrell,
F-actin buckling coordinates contractility and severing in a biomimetic actomyosin cortex.
2012,
Pubmed
Nair,
Using patterned supported lipid membranes to investigate the role of receptor organization in intercellular signaling.
2011,
Pubmed
Nguyen,
Spatial organization of cytokinesis signaling reconstituted in a cell-free system.
2014,
Pubmed
,
Xenbase
Popp,
Site-specific protein labeling via sortase-mediated transpeptidation.
2009,
Pubmed
Richter,
Formation of solid-supported lipid bilayers: an integrated view.
2006,
Pubmed
Riedl,
Lifeact: a versatile marker to visualize F-actin.
2008,
Pubmed
Roubinet,
Common mechanisms regulating cell cortex properties during cell division and cell migration.
2012,
Pubmed
Sackmann,
Supported membranes on soft polymer cushions: fabrication, characterization and applications.
2000,
Pubmed
Sackmann,
Supported membranes: scientific and practical applications.
1996,
Pubmed
Sawin,
Mitotic spindle assembly by two different pathways in vitro.
1991,
Pubmed
,
Xenbase
Schweizer,
Geometry sensing by self-organized protein patterns.
2012,
Pubmed
Tanaka,
Polymer-supported membranes as models of the cell surface.
2005,
Pubmed
Tsai,
Aurora A kinase-coated beads function as microtubule-organizing centers and enhance RanGTP-induced spindle assembly.
2005,
Pubmed
,
Xenbase
Visnapuu,
Single-molecule imaging of DNA curtains reveals intrinsic energy landscapes for nucleosome deposition.
2009,
Pubmed
Vogel,
Myosin motors fragment and compact membrane-bound actin filaments.
2013,
Pubmed
Wagner,
Tethered polymer-supported planar lipid bilayers for reconstitution of integral membrane proteins: silane-polyethyleneglycol-lipid as a cushion and covalent linker.
2000,
Pubmed
Zieske,
Reconstitution of pole-to-pole oscillations of min proteins in microengineered polydimethylsiloxane compartments.
2013,
Pubmed
