Agricola ZN , Jagpal AK , Allbee AW , Prewitt AR , Shifley ET , Rankin SA , Zorn AM , Kenny AP .

R01 DK070858 NIDDK NIH HHS , P30 DK078392 NIDDK NIH HHS , R01 HD073179 NICHD NIH HHS , T32 HD007463 NICHD NIH HHS , T32 HD07463 NICHD NIH HHS , R01 HD73179 NICHD NIH HHS , K08 HL105661 NHLBI NIH HHS

Xla Wt + cebpa (Fig.4.b^1)
Xla Wt + cebpa (Fig.4.h^1)
Xla Wt + cebpa (Fig.4.i^1)
Xla Wt + spib MO (Fig.3.a^1)
Xla Wt + spib MO (Fig.3.b^1)
Xla Wt + spib MO (Fig.3.c^1)
Xla Wt + spib MO (Fig.3.d^1)
Xla Wt + spib MO (Fig.3.e^1)
Xla Wt + spib MO (Fig.3.f^1)
Xla Wt + spib MO (Fig.3.g^1)
Xla Wt + spib MO (Fig.3.h^1)
Xla Wt + spib MO (Fig.3.i^1)
Xla Wt + spib MO (Fig.3.j^1)
Xla Wt + spib MO (Fig.5.b)
Xla Wt + spib MO (Fig.5.e)

	Figure 1. Stage 15, 19, 24, and 28 expression of identified genes progress from anterior ventral blood island to dispersed distribution. They are temporally downstream of transcriptional regulators spib.a (A–A‴) but coincident with other, later expressing myeloid functional genes like mmp7 (B–B‴). C–C‴, adam28. D–D‴, coro1a. E–E‴, ctss. F–F‴, dstn. G–G‴, fmnl1. H–H‴, ptpn6. I–I‴, timp-1. White scale bar = 1 mm.
	Figure 2. Sequential double in situ hybridization reveals target gene expression corresponds with increased myeloid cell motility. A: Stage 22 inner view. A′, spib.a only (BCIP blue) initial in situ. A′: follow-up image after second probe for destrin reveals significant overlap of two genes. B–B′: outer view of same embryo. C and D, increased motility in destrin-expressing cells compared with spib.a-expressing cells (C′ and D′, ventral views).
	Figure 3. Knockdown of primitive myeloid lineage spib.a transcription factor results in loss of myeloid gene candidate expression in the anterior ventral blood island (aVBI) of stage 23 embryos. A–J: Normal expression of genes in the aVBI of uninjected control embryos. A′–J′: Reduced aVBI expression of genes in the embryos injected dorsally at 4-cell stage with 40 ng e1i1 spib.a morpholino. A+A′, spib.a. B+B′, mpo. C+C′, mmp7. D+D′, adam28. E+E′, coro1a. F+F′, ctss. G+G′, dstn. H+H′, fmnl1. I+I′, timp-1. J+J′, ptpn6.
	Figure 4. Animal cap-injected X.t. cebpa mRNA induces exogenous myeloid gene expression in Stage 21 X.l. embryos. A–J: Uninjected embryos ventral view. A′–J′: 200 pg cebpa and 300 pg gfp mRNA coinjected embryos ventral view. A, A′: spib.a. B, B′: mpo. C, C′: mmp7. D, D′: dstn. E, E′: coro1a. F, F′: ptpn6. G, G′: timp–1. H, H′: fmnl1. I, I′: ctss. J, J′: adam28.
	Figure 5. cebpa animal cap Einsteck rescue of dstn (A–C) and coro1a (D–F) expression spib.a-MO myeloid-depleted host embryos (Stage 21 ventral views). A,D: uninjected control embryos. B,E: spib.a-MO myeloid depleted host embryos. C,F: cebpa animal cap transplanted by means of Einsteck into stage 10 blastocoeles of myeloid-depleted hosts.Download figure to PowerPoint
	adam28 (ADAM metallopeptidase domain 28) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 24, ventral view, anterior left
	coro1a (coronin, actin binding protein, 1A ) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 24, ventral view, anterior left.
	ctss-a (cathepsin S) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 24, ventral view, anterior left.
	dstn (destrin) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 24, ventral view, anterior left.
	fmnl1 (formin like 1) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 24, ventral view, anterior left.
	timp-1 (tissue inhibitor of metalloproteinase-1) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 24, ventral view, anterior left.

Arnold, Inflammatory monocytes recruited after skeletal muscle injury switch into antiinflammatory macrophages to support myogenesis. 2007, Pubmed

Arnold, Inflammatory monocytes recruited after skeletal muscle injury switch into antiinflammatory macrophages to support myogenesis. 2007, Pubmed
Arora, Expression of protein-tyrosine phosphatases in Acute Myeloid Leukemia cells: FLT3 ITD sustains high levels of DUSP6 expression. 2012, Pubmed
Banaei-Bouchareb, Insulin cell mass is altered in Csf1op/Csf1op macrophage-deficient mice. 2004, Pubmed
Banaei-Bouchareb, A transient microenvironment loaded mainly with macrophages in the early developing human pancreas. 2006, Pubmed
Bonnans, Remodelling the extracellular matrix in development and disease. 2014, Pubmed
Castro-Castro, Coronin 1A promotes a cytoskeletal-based feedback loop that facilitates Rac1 translocation and activation. 2011, Pubmed
Chen, ToppGene Suite for gene list enrichment analysis and candidate gene prioritization. 2009, Pubmed
Chen, C/EBPalpha initiates primitive myelopoiesis in pluripotent embryonic cells. 2009, Pubmed , Xenbase
Ciau-Uitz, Developmental hematopoiesis: ontogeny, genetic programming and conservation. 2014, Pubmed , Xenbase
Ciau-Uitz, Genetic control of hematopoietic development in Xenopus and zebrafish. 2010, Pubmed , Xenbase
Costa, spib is required for primitive myeloid development in Xenopus. 2008, Pubmed , Xenbase
Diez-Roux, Macrophages kill capillary cells in G1 phase of the cell cycle during programmed vascular regression. 1999, Pubmed
Duffield, Selective depletion of macrophages reveals distinct, opposing roles during liver injury and repair. 2005, Pubmed
Federzoni, The actin-binding protein CORO1A is a novel PU.1 (SPI1)- and CEBPA-regulated gene with significantly lower expression in APL and CEBPA-mutated AML patients. 2013, Pubmed
Franc, Requirement for croquemort in phagocytosis of apoptotic cells in Drosophila. 1999, Pubmed
Gibb, The emergence of ADAM10 as a regulator of lymphocyte development and autoimmunity. 2011, Pubmed
Goren, A transgenic mouse model of inducible macrophage depletion: effects of diphtheria toxin-driven lysozyme M-specific cell lineage ablation on wound inflammatory, angiogenic, and contractive processes. 2009, Pubmed
Göttgens, Transcription of the SCL gene in erythroid and CD34 positive primitive myeloid cells is controlled by a complex network of lineage-restricted chromatin-dependent and chromatin-independent regulatory elements. 1997, Pubmed
Guedez, TIMP-2 targets tumor-associated myeloid suppressor cells with effects in cancer immune dysfunction and angiogenesis. 2012, Pubmed
Guo, ADAM10 overexpression in human non-small cell lung cancer correlates with cell migration and invasion through the activation of the Notch1 signaling pathway. 2012, Pubmed
Harrison, Matrix metalloproteinase genes in Xenopus development. 2004, Pubmed , Xenbase
Hatanaka, Tertiary structure of destrin and structural similarity between two actin-regulating protein families. 1996, Pubmed
Ichikawa, Differentiation of myeloid leukemia cells and changes in their contractile proteins. 1982, Pubmed
Jones, Cellular signaling in macrophage migration and chemotaxis. 2000, Pubmed
Kau, Dual contribution of embryonic ventral blood island and dorsal lateral plate mesoderm during ontogeny of hemopoietic cells in Xenopus laevis. 1983, Pubmed , Xenbase
Kenny, Sizzled-tolloid interactions maintain foregut progenitors by regulating fibronectin-dependent BMP signaling. 2012, Pubmed , Xenbase
Khokha, Metalloproteinases and their natural inhibitors in inflammation and immunity. 2013, Pubmed
Kitzing, Formin-like 2 drives amoeboid invasive cell motility downstream of RhoC. 2010, Pubmed
Lauriol, The role of the protein tyrosine phosphatase SHP2 in cardiac development and disease. 2015, Pubmed
Lin, Macrophage Wnt7b is critical for kidney repair and regeneration. 2010, Pubmed , Xenbase
Lux, All primitive and definitive hematopoietic progenitor cells emerging before E10 in the mouse embryo are products of the yolk sac. 2008, Pubmed
Maéno, Differential participation of ventral and dorsolateral mesoderms in the hemopoiesis of Xenopus, as revealed in diploid-triploid or interspecific chimeras. 1985, Pubmed , Xenbase
McComb, Cathepsins limit macrophage necroptosis through cleavage of Rip1 kinase. 2014, Pubmed
McGrath, Hematopoiesis in the yolk sac: more than meets the eye. 2005, Pubmed
McLin, Repression of Wnt/beta-catenin signaling in the anterior endoderm is essential for liver and pancreas development. 2007, Pubmed , Xenbase
Meijer, Kupffer cell depletion by CI2MDP-liposomes alters hepatic cytokine expression and delays liver regeneration after partial hepatectomy. 2000, Pubmed
Mersich, The formin FRL1 (FMNL1) is an essential component of macrophage podosomes. 2010, Pubmed
Mirza, Selective and specific macrophage ablation is detrimental to wound healing in mice. 2009, Pubmed
Mueller, C/EBPalpha and the pathophysiology of acute myeloid leukemia. 2006, Pubmed
Nagata, Electron microscopic study on the early histogenesis of thymus in the toad, Xenopus laevis. 1977, Pubmed , Xenbase
Nahrendorf, The healing myocardium sequentially mobilizes two monocyte subsets with divergent and complementary functions. 2007, Pubmed
Nerlov, C/EBPalpha mutations in acute myeloid leukaemias. 2004, Pubmed
Palis, Development of erythroid and myeloid progenitors in the yolk sac and embryo proper of the mouse. 1999, Pubmed
Pull, Activated macrophages are an adaptive element of the colonic epithelial progenitor niche necessary for regenerative responses to injury. 2005, Pubmed
Rae, Characterisation and trophic functions of murine embryonic macrophages based upon the use of a Csf1r-EGFP transgene reporter. 2007, Pubmed
Rochelle, p210bcr-abl induces amoeboid motility by recruiting ADF/destrin through RhoA/ROCK1. 2013, Pubmed
Savill, Corpse clearance defines the meaning of cell death. 2000, Pubmed
Seno, Efficient colonic mucosal wound repair requires Trem2 signaling. 2009, Pubmed
Seto, Coronin-1a inhibits autophagosome formation around Mycobacterium tuberculosis-containing phagosomes and assists mycobacterial survival in macrophages. 2012, Pubmed
Shimoda, Binding of ADAM28 to P-selectin glycoprotein ligand-1 enhances P-selectin-mediated leukocyte adhesion to endothelial cells. 2007, Pubmed
Sidney, Concise review: evidence for CD34 as a common marker for diverse progenitors. 2014, Pubmed
Smith, XPOX2-peroxidase expression and the XLURP-1 promoter reveal the site of embryonic myeloid cell development in Xenopus. 2002, Pubmed , Xenbase
Smith, Early cardiac morphogenesis defects caused by loss of embryonic macrophage function in Xenopus. 2011, Pubmed , Xenbase
Stefater, Metchnikoff's policemen: macrophages in development, homeostasis and regeneration. 2011, Pubmed
Suetsugu, Regulation of cortical actin networks in cell migration. 2003, Pubmed
Takeishi, The role of Kupffer cells in liver regeneration. 1999, Pubmed
Teng, Conditional Knockout of Src Homology 2 Domain-containing Protein Tyrosine Phosphatase-2 in Myeloid Cells Attenuates Renal Fibrosis after Unilateral Ureter Obstruction. 2015, Pubmed
Tepass, Embryonic origin of hemocytes and their relationship to cell death in Drosophila. 1994, Pubmed
Tochinai, Direct observation of cell migration into Xenopus thymus rudiments through mesenchyme. 1980, Pubmed , Xenbase
van der Vorst, Myeloid A disintegrin and metalloproteinase domain 10 deficiency modulates atherosclerotic plaque composition by shifting the balance from inflammation toward fibrosis. 2015, Pubmed
Vérollet, Extracellular proteolysis in macrophage migration: losing grip for a breakthrough. 2011, Pubmed
Wakayama, Cdc42 mediates Bmp-induced sprouting angiogenesis through Fmnl3-driven assembly of endothelial filopodia in zebrafish. 2015, Pubmed
Warga, Fate mapping embryonic blood in zebrafish: multi- and unipotential lineages are segregated at gastrulation. 2009, Pubmed
Yan, Coronin-1 function is required for phagosome formation. 2005, Pubmed
Zajac, Angiogenic capacity of M1- and M2-polarized macrophages is determined by the levels of TIMP-1 complexed with their secreted proMMP-9. 2013, Pubmed
Zhang, Enhancement of hematopoietic stem cell repopulating capacity and self-renewal in the absence of the transcription factor C/EBP alpha. 2004, Pubmed