Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
???displayArticle.abstract???
We have isolated the complete coding sequences for two Xenopus laevis isoforms of heterochromatin protein 1, corresponding to HP1alpha and HP1gamma. The sequence of xHP1alpha shows considerable divergence from its mammalian homologues, whereas xHP1gamma is highly conserved. Functionally, xHP1alpha behaves identically to human HP1alpha. We observe unexpected differences between the two HP1 variants in binding native soluble chromatin, which seem to correlate with their distinct nuclear distributions in vivo. A surprising finding is that the characteristic interaction of HP1 chromodomains with histone H3 at methylated lysine 9 is not detected in preformed chromatin due to its inaccessibility. Instead, we localize a strong chromatin-binding activity to the short hinge region between the chromodomain and the chromoshadow domain of xHP1alpha but not xHP1gamma. This novel chromatin-binding activity has a non-specific DNA-binding component in addition to a linker histone-dependent preference for an altered chromatin structure with a likely heterochromatin organization.
Angelov,
Preferential interaction of the core histone tail domains with linker DNA.
2001, Pubmed
Angelov,
Preferential interaction of the core histone tail domains with linker DNA.
2001,
Pubmed
Bannister,
Selective recognition of methylated lysine 9 on histone H3 by the HP1 chromo domain.
2001,
Pubmed
Bates,
Histones H1 and H5: one or two molecules per nucleosome?
1981,
Pubmed
Cary,
High-resolution proton-magnetic-resonance studies of chromatin core particles.
1978,
Pubmed
Coles,
Characterization of the chicken histone H1 gene complement. Generation of a complete set of vertebrate H1 protein sequences.
1987,
Pubmed
Cowell,
Self-association of chromo domain peptides.
1997,
Pubmed
Cowell,
Heterochromatin, HP1 and methylation at lysine 9 of histone H3 in animals.
2002,
Pubmed
Eissenberg,
Mutation in a heterochromatin-specific chromosomal protein is associated with suppression of position-effect variegation in Drosophila melanogaster.
1990,
Pubmed
Hong,
Studies of the DNA binding properties of histone H4 amino terminus. Thermal denaturation studies reveal that acetylation markedly reduces the binding constant of the H4 "tail" to DNA.
1993,
Pubmed
Jacobs,
Structure of HP1 chromodomain bound to a lysine 9-methylated histone H3 tail.
2002,
Pubmed
James,
Distribution patterns of HP1, a heterochromatin-associated nonhistone chromosomal protein of Drosophila.
1989,
Pubmed
Jenuwein,
Translating the histone code.
2001,
Pubmed
Lachner,
Methylation of histone H3 lysine 9 creates a binding site for HP1 proteins.
2001,
Pubmed
Li,
Does heterochromatin protein 1 always follow code?
2002,
Pubmed
Muchardt,
Coordinated methyl and RNA binding is required for heterochromatin localization of mammalian HP1alpha.
2002,
Pubmed
Nielsen,
Heterochromatin formation in mammalian cells: interaction between histones and HP1 proteins.
2001,
Pubmed
Nielsen,
Structure of the HP1 chromodomain bound to histone H3 methylated at lysine 9.
2002,
Pubmed
Pak,
Association of the origin recognition complex with heterochromatin and HP1 in higher eukaryotes.
1997,
Pubmed
,
Xenbase
Platero,
Functional analysis of the chromo domain of HP1.
1995,
Pubmed
Richards,
Epigenetic codes for heterochromatin formation and silencing: rounding up the usual suspects.
2002,
Pubmed
Smothers,
The hinge and chromo shadow domain impart distinct targeting of HP1-like proteins.
2001,
Pubmed
Sugimoto,
Human homolog of Drosophila heterochromatin-associated protein 1 (HP1) is a DNA-binding protein which possesses a DNA-binding motif with weak similarity to that of human centromere protein C (CENP-C).
1996,
Pubmed
Wang,
Conservation of heterochromatin protein 1 function.
2000,
Pubmed
Weintraub,
The generation and propagation of variegated chromosome structures.
1978,
Pubmed
Yamada,
Functional domain structure of human heterochromatin protein HP1(Hsalpha): involvement of internal DNA-binding and C-terminal self-association domains in the formation of discrete dots in interphase nuclei.
1999,
Pubmed
Yamaguchi,
Chicken chromobox proteins: cDNA cloning of CHCB1, -2, -3 and their relation to W-heterochromatin.
1998,
Pubmed
,
Xenbase
Zhao,
Heterochromatin protein 1 binds to nucleosomes and DNA in vitro.
2000,
Pubmed
