Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
J Appl Physiol (1985)
2016 May 15;12010:1260-6. doi: 10.1152/japplphysiol.01060.2015.
Show Gene links
Show Anatomy links
Cytosolic calcium transients are a determinant of contraction-induced HSP72 transcription in single skeletal muscle fibers.
Stary CM
,
Hogan MC
.
???displayArticle.abstract???
The intrinsic activating factors that induce transcription of heat shock protein 72 (HSP72) in skeletal muscle following exercise remain unclear. We hypothesized that the cytosolic Ca(2+) transient that occurs with depolarization is a determinant. We utilized intact, single skeletal muscle fibers from Xenopus laevis to test the role of the cytosolic Ca(2+) transient and several other exercise-related factors (fatigue, hypoxia, AMP kinase, and cross-bridge cycling) on the activation of HSP72 transcription. HSP72 and HSP60 mRNA levels were assessed with real-time quantitative PCR; cytosolic Ca(2+) concentration ([Ca(2+)]cyt) was assessed with fura-2. Both fatiguing and nonfatiguing contractions resulted in a significant increase in HSP72 mRNA. As expected, peak [Ca(2+)]cyt remained tightly coupled with peak developed tension in contracting fibers. Pretreatment with N-benzyl-p-toluene sulfonamide (BTS) resulted in depressed peak developed tension with stimulation, while peak [Ca(2+)]cyt remained largely unchanged from control values. Despite excitation-contraction uncoupling, BTS-treated fibers displayed a significant increase in HSP72 mRNA. Treatment of fibers with hypoxia (Po2: <3 mmHg) or AMP kinase activation had no effect on HSP72 mRNA levels. These results suggest that the intermittent cytosolic Ca(2+) transient that occurs with skeletal muscle depolarization provides a sufficient activating stimulus for HSP72 transcription. Metabolic or mechanical factors associated with fatigue development and cross-bridge cycling likely play a more limited role.
Abruzzo,
Moderate exercise training induces ROS-related adaptations to skeletal muscles.
2013, Pubmed
Abruzzo,
Moderate exercise training induces ROS-related adaptations to skeletal muscles.
2013,
Pubmed
Amorim,
The effect of the rate of heat storage on serum heat shock protein 72 in humans.
2008,
Pubmed
Barash,
Rapid muscle-specific gene expression changes after a single bout of eccentric contractions in the mouse.
2004,
Pubmed
Breen,
Angiogenic growth factor mRNA responses in muscle to a single bout of exercise.
1996,
Pubmed
Carmeli,
Increased iNOS, MMP-2, and HSP-72 in skeletal muscle following high-intensity exercise training.
2010,
Pubmed
Chin,
The role of calcium and calcium/calmodulin-dependent kinases in skeletal muscle plasticity and mitochondrial biogenesis.
2004,
Pubmed
Connor,
Contractile activity-induced transcriptional activation of cytochrome C involves Sp1 and is proportional to mitochondrial ATP synthesis in C2C12 muscle cells.
2001,
Pubmed
Dodd,
Hsp70 prevents disuse muscle atrophy in senescent rats.
2009,
Pubmed
Egawa,
AICAR-induced activation of AMPK negatively regulates myotube hypertrophy through the HSP72-mediated pathway in C2C12 skeletal muscle cells.
2014,
Pubmed
Febbraio,
HSP72 gene expression progressively increases in human skeletal muscle during prolonged, exhaustive exercise.
2000,
Pubmed
Fediuc,
Regulation of AMP-activated protein kinase and acetyl-CoA carboxylase phosphorylation by palmitate in skeletal muscle cells.
2006,
Pubmed
Fischer,
Vitamin E isoform-specific inhibition of the exercise-induced heat shock protein 72 expression in humans.
2006,
Pubmed
Fitts,
Cellular mechanisms of muscle fatigue.
1994,
Pubmed
Gehrig,
Hsp72 preserves muscle function and slows progression of severe muscular dystrophy.
2012,
Pubmed
Gibson,
Extracellular Hsp72 concentration relates to a minimum endogenous criteria during acute exercise-heat exposure.
2014,
Pubmed
Giffard,
Regulation of apoptotic and inflammatory cell signaling in cerebral ischemia: the complex roles of heat shock protein 70.
2008,
Pubmed
Hellsten,
Antioxidant supplementation enhances the exercise-induced increase in mitochondrial uncoupling protein 3 and endothelial nitric oxide synthase mRNA content in human skeletal muscle.
2007,
Pubmed
Henstridge,
Heat shock proteins and exercise adaptations. Our knowledge thus far and the road still ahead.
2016,
Pubmed
Henstridge,
Activating HSP72 in rodent skeletal muscle increases mitochondrial number and oxidative capacity and decreases insulin resistance.
2014,
Pubmed
Hogan,
Fall in intracellular PO(2) at the onset of contractions in Xenopus single skeletal muscle fibers.
2001,
Pubmed
,
Xenbase
Hogan,
Phosphorescence quenching method for measurement of intracellular PO2 in isolated skeletal muscle fibers.
1999,
Pubmed
,
Xenbase
Hogan,
NAD(P)H fluorescence imaging of mitochondrial metabolism in contracting Xenopus skeletal muscle fibers: effect of oxygen availability.
2005,
Pubmed
,
Xenbase
Hultman,
Skeletal muscle energy metabolism and fatigue during intense exercise in man.
1991,
Pubmed
Hutber,
Electrical stimulation inactivates muscle acetyl-CoA carboxylase and increases AMP-activated protein kinase.
1997,
Pubmed
Jo,
Heat preconditioning attenuates renal injury in ischemic ARF in rats: role of heat-shock protein 70 on NF-kappaB-mediated inflammation and on tubular cell injury.
2006,
Pubmed
Johnson,
Adrenergic receptors mediate stress-induced elevations in extracellular Hsp72.
2005,
Pubmed
Jorquera,
Membrane depolarization induces calcium-dependent upregulation of Hsp70 and Hmox-1 in skeletal muscle cells.
2009,
Pubmed
Kim,
The effect of exercise in cool, control and hot environments on cardioprotective HSP70 induction.
2004,
Pubmed
Kinnunen,
Exercise-induced oxidative stress and muscle stress protein responses in trotters.
2005,
Pubmed
Liu,
Different skeletal muscle HSP70 responses to high-intensity strength training and low-intensity endurance training.
2004,
Pubmed
Livak,
Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method.
2001,
Pubmed
Locke,
Heat shock protein accumulation and heat shock transcription factor activation in rat skeletal muscle during compensatory hypertrophy.
2008,
Pubmed
Lännergren,
Force relaxation, labile heat and parvalbumin content of skeletal muscle fibres of Xenopus laevis.
1993,
Pubmed
,
Xenbase
McArdle,
Overexpression of HSP70 in mouse skeletal muscle protects against muscle damage and age-related muscle dysfunction.
2004,
Pubmed
Milne,
Exercise-induced elevation of HSP70 is intensity dependent.
2002,
Pubmed
Miyabara,
Overexpression of inducible 70-kDa heat shock protein in mouse attenuates skeletal muscle damage induced by cryolesioning.
2006,
Pubmed
Morton,
The exercise-induced stress response of skeletal muscle, with specific emphasis on humans.
2009,
Pubmed
Nagesser,
Metabolic changes with fatigue in different types of single muscle fibres of Xenopus laevis.
1992,
Pubmed
,
Xenbase
Petersen,
Infusion with the antioxidant N-acetylcysteine attenuates early adaptive responses to exercise in human skeletal muscle.
2012,
Pubmed
Powers,
Exercise, heat shock proteins, and myocardial protection from I-R injury.
2001,
Pubmed
Puntschart,
Hsp70 expression in human skeletal muscle after exercise.
1996,
Pubmed
Pösö,
Induction of heat shock protein 72 mRNA in skeletal muscle by exercise and training.
2002,
Pubmed
Reid,
Nitric oxide, reactive oxygen species, and skeletal muscle contraction.
2001,
Pubmed
Salo,
HSP70 and other possible heat shock or oxidative stress proteins are induced in skeletal muscle, heart, and liver during exercise.
1991,
Pubmed
Sandström,
Mechanical load plays little role in contraction-mediated glucose transport in mouse skeletal muscle.
2007,
Pubmed
Skidmore,
HSP70 induction during exercise and heat stress in rats: role of internal temperature.
1995,
Pubmed
Stary,
Impairment of Ca(2+) release in single Xenopus muscle fibers fatigued at varied extracellular PO(2).
2000,
Pubmed
,
Xenbase
Stary,
Resistance to fatigue of individual Xenopus single skeletal muscle fibres is correlated with mitochondrial volume density.
2004,
Pubmed
,
Xenbase
Stary,
Elevation in heat shock protein 72 mRNA following contractions in isolated single skeletal muscle fibers.
2008,
Pubmed
,
Xenbase
Stoppani,
AMP-activated protein kinase activates transcription of the UCP3 and HKII genes in rat skeletal muscle.
2002,
Pubmed
Tang,
HIF and VEGF relationships in response to hypoxia and sciatic nerve stimulation in rat gastrocnemius.
2004,
Pubmed
Thompson,
A single bout of eccentric exercise increases HSP27 and HSC/HSP70 in human skeletal muscle.
2001,
Pubmed
Thompson,
Exercise-induced HSP27, HSP70 and MAPK responses in human skeletal muscle.
2003,
Pubmed
Walsh,
Exercise increases serum Hsp72 in humans.
2001,
Pubmed
Walsh,
Measurement of activation energy and oxidative phosphorylation onset kinetics in isolated muscle fibers in the absence of cross-bridge cycling.
2006,
Pubmed
,
Xenbase
Westerblad,
Muscle fatigue: lactic acid or inorganic phosphate the major cause?
2002,
Pubmed
Westerblad,
Cellular mechanisms of skeletal muscle fatigue.
2003,
Pubmed
Westerblad,
Cellular mechanisms of fatigue in skeletal muscle.
1991,
Pubmed
Whitham,
Effect of exercise with and without a thermal clamp on the plasma heat shock protein 72 response.
2007,
Pubmed
Zuo,
Reactive oxygen species formation in the transition to hypoxia in skeletal muscle.
2005,
Pubmed
van der Laarse,
Resistance to fatigue of single muscle fibres from Xenopus related to succinate dehydrogenase and myofibrillar ATPase activities.
1991,
Pubmed
,
Xenbase