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Warzecha CC
,
Sato TK
,
Nabet B
,
Hogenesch JB
,
Carstens RP
.
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Cell-type-specific expression of epithelial and mesenchymal isoforms of Fibroblast Growth Factor Receptor 2 (FGFR2) is achieved through tight regulation of mutually exclusive exons IIIb and IIIc, respectively. Using an application of cell-based cDNA expression screening, we identified two paralogous epithelial cell-type-specific RNA-binding proteins that are essential regulators of FGFR2 splicing. Ectopic expression of either protein in cells that express FGFR2-IIIc caused a switch in endogenous FGFR2 splicing to the epithelial isoform. Conversely, knockdown of both factors in cells that express FGFR2-IIIb by RNA interference caused a switch from the epithelial to mesenchymal isoform. These factors also regulate splicing of CD44, p120-Catenin (CTNND1), and hMena (ENAH), three transcripts that undergo changes in splicing during the epithelial-to-mesenchymal transition (EMT). These studies suggest that Epithelial Splicing Regulatory Proteins 1 and 2 (ESRP1 and ESRP2) are coordinators of an epithelial cell-type-specific splicing program.
Allemand,
Regulation of heterogenous nuclear ribonucleoprotein A1 transport by phosphorylation in cells stressed by osmotic shock.
2005, Pubmed
Allemand,
Regulation of heterogenous nuclear ribonucleoprotein A1 transport by phosphorylation in cells stressed by osmotic shock.
2005,
Pubmed
Baraniak,
Fox-2 mediates epithelial cell-specific fibroblast growth factor receptor 2 exon choice.
2006,
Pubmed
Barberan-Soler,
Alternative splicing regulation during C. elegans development: splicing factors as regulated targets.
2008,
Pubmed
Black,
Mechanisms of alternative pre-messenger RNA splicing.
2003,
Pubmed
Boutz,
MicroRNAs regulate the expression of the alternative splicing factor nPTB during muscle development.
2007,
Pubmed
Cha,
Involvement of fibroblast growth factor receptor 2 isoform switching in mammary oncogenesis.
2008,
Pubmed
David,
The search for alternative splicing regulators: new approaches offer a path to a splicing code.
2008,
Pubmed
Eswarakumar,
Cellular signaling by fibroblast growth factor receptors.
2005,
Pubmed
Hanamura,
Regulated tissue-specific expression of antagonistic pre-mRNA splicing factors.
1998,
Pubmed
Heyd,
Getting under the skin of alternative splicing: identification of epithelial-specific splicing factors.
2009,
Pubmed
Hovhannisyan,
Characterization of sequences and mechanisms through which ISE/ISS-3 regulates FGFR2 splicing.
2006,
Pubmed
Hovhannisyan,
Heterogeneous ribonucleoprotein m is a splicing regulatory protein that can enhance or silence splicing of alternatively spliced exons.
2007,
Pubmed
Hovhannisyan,
A novel intronic cis element, ISE/ISS-3, regulates rat fibroblast growth factor receptor 2 splicing through activation of an upstream exon and repression of a downstream exon containing a noncanonical branch point sequence.
2005,
Pubmed
Huang,
Epithelial to mesenchymal transition in human breast epithelial cells transformed by 17beta-estradiol.
2007,
Pubmed
Kar,
RBM4 interacts with an intronic element and stimulates tau exon 10 inclusion.
2006,
Pubmed
Keene,
RNA regulons: coordination of post-transcriptional events.
2007,
Pubmed
Keirsebilck,
Molecular cloning of the human p120ctn catenin gene (CTNND1): expression of multiple alternatively spliced isoforms.
1998,
Pubmed
Kuroyanagi,
The Fox-1 family and SUP-12 coordinately regulate tissue-specific alternative splicing in vivo.
2007,
Pubmed
Li,
Neuronal regulation of alternative pre-mRNA splicing.
2007,
Pubmed
Luesch,
Towards high-throughput characterization of small molecule mechanisms of action.
2006,
Pubmed
Makeyev,
The MicroRNA miR-124 promotes neuronal differentiation by triggering brain-specific alternative pre-mRNA splicing.
2007,
Pubmed
Matlin,
Understanding alternative splicing: towards a cellular code.
2005,
Pubmed
Mauger,
hnRNP H and hnRNP F complex with Fox2 to silence fibroblast growth factor receptor 2 exon IIIc.
2008,
Pubmed
McKee,
A genome-wide in situ hybridization map of RNA-binding proteins reveals anatomically restricted expression in the developing mouse brain.
2005,
Pubmed
Muh,
A Non-sequence-specific double-stranded RNA structural element regulates splicing of two mutually exclusive exons of fibroblast growth factor receptor 2 (FGFR2).
2002,
Pubmed
,
Xenbase
Newman,
Identification of RNA-binding proteins that regulate FGFR2 splicing through the use of sensitive and specific dual color fluorescence minigene assays.
2006,
Pubmed
Oberdoerffer,
Regulation of CD45 alternative splicing by heterogeneous ribonucleoprotein, hnRNPLL.
2008,
Pubmed
Ohkubo,
The transcription factor Snail downregulates the tight junction components independently of E-cadherin downregulation.
2004,
Pubmed
Onder,
Loss of E-cadherin promotes metastasis via multiple downstream transcriptional pathways.
2008,
Pubmed
Orr-Urtreger,
Developmental localization of the splicing alternatives of fibroblast growth factor receptor-2 (FGFR2).
1993,
Pubmed
Park,
The miR-200 family determines the epithelial phenotype of cancer cells by targeting the E-cadherin repressors ZEB1 and ZEB2.
2008,
Pubmed
Pino,
Human Mena+11a isoform serves as a marker of epithelial phenotype and sensitivity to epidermal growth factor receptor inhibition in human pancreatic cancer cell lines.
2008,
Pubmed
Ponta,
CD44: from adhesion molecules to signalling regulators.
2003,
Pubmed
Sherman,
A splice variant of CD44 expressed in the apical ectodermal ridge presents fibroblast growth factors to limb mesenchyme and is required for limb outgrowth.
1998,
Pubmed
Smith,
Alternative pre-mRNA splicing: the logic of combinatorial control.
2000,
Pubmed
Stamm,
Regulation of alternative splicing by reversible protein phosphorylation.
2008,
Pubmed
Thiery,
Complex networks orchestrate epithelial-mesenchymal transitions.
2006,
Pubmed
Topp,
A cell-based screen for splicing regulators identifies hnRNP LL as a distinct signal-induced repressor of CD45 variable exon 4.
2008,
Pubmed
Ule,
Nova regulates brain-specific splicing to shape the synapse.
2005,
Pubmed
Visel,
GenePaint.org: an atlas of gene expression patterns in the mouse embryo.
2004,
Pubmed
Wakabayashi-Ito,
fusilli, an essential gene with a maternal role in Drosophila embryonic dorsal-ventral patterning.
2001,
Pubmed
Wu,
SRp54 (SFRS11), a regulator for tau exon 10 alternative splicing identified by an expression cloning strategy.
2006,
Pubmed
Xu,
Fibroblast growth factor receptor 2 (FGFR2)-mediated reciprocal regulation loop between FGF8 and FGF10 is essential for limb induction.
1998,
Pubmed
Yanagisawa,
A p120 catenin isoform switch affects Rho activity, induces tumor cell invasion, and predicts metastatic disease.
2008,
Pubmed
Yang,
Twist, a master regulator of morphogenesis, plays an essential role in tumor metastasis.
2004,
Pubmed
Yang,
Epithelial-mesenchymal transition: at the crossroads of development and tumor metastasis.
2008,
Pubmed
Zhang,
Defining the regulatory network of the tissue-specific splicing factors Fox-1 and Fox-2.
2008,
Pubmed
Zhang,
Receptor specificity of the fibroblast growth factor family. The complete mammalian FGF family.
2006,
Pubmed
