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EMBO J
2003 Jun 02;2211:2841-51. doi: 10.1093/emboj/cdg249.
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Coordinated nuclear export of 60S ribosomal subunits and NMD3 in vertebrates.
Trotta CR
,
Lund E
,
Kahan L
,
Johnson AW
,
Dahlberg JE
.
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60S and 40S ribosomal subunits are assembled in the nucleolus and exported from the nucleus to the cytoplasm independently of each other. We show that in vertebrate cells, transport of both subunits requires the export receptor CRM1 and Ran.GTP. Export of 60S subunits is coupled with that of the nucleo- cytoplasmic shuttling protein NMD3. Human NMD3 (hNMD3) contains a CRM-1-dependent leucine-rich nuclear export signal (NES) and a complex, dispersed nuclear localization signal (NLS), the basic region of which is also required for nucleolar accumulation. When present in Xenopus oocytes, both wild-type and export-defective mutant hNMD3 proteins bind to newly made nuclear 60S pre-export particles at a late step of subunit maturation. The export-defective hNMD3, but not the wild-type protein, inhibits export of 60S subunits from oocyte nuclei. These results indicate that the NES mutant protein competes with endogenous wild-type frog NMD3 for binding to nascent 60S subunits, thereby preventing their export. We propose that NMD3 acts as an adaptor for CRM1-Ran.GTP-mediated 60S subunit export, by a mechanism that is conserved from vertebrates to yeast.
Allison,
RNA-protein interactions of stored 5S RNA with TFIIIA and ribosomal protein L5 during Xenopus oogenesis.
1991, Pubmed,
Xenbase
Allison,
RNA-protein interactions of stored 5S RNA with TFIIIA and ribosomal protein L5 during Xenopus oogenesis.
1991,
Pubmed
,
Xenbase
Andersen,
Directed proteomic analysis of the human nucleolus.
2002,
Pubmed
Askjaer,
RanGTP-regulated interactions of CRM1 with nucleoporins and a shuttling DEAD-box helicase.
1999,
Pubmed
,
Xenbase
Bataillé,
Cytoplasmic transport of ribosomal subunits microinjected into the Xenopus laevis oocyte nucleus: a generalized, facilitated process.
1990,
Pubmed
,
Xenbase
Belk,
Overexpression of truncated Nmd3p inhibits protein synthesis in yeast.
1999,
Pubmed
Boulikas,
Nuclear localization signals (NLS).
1993,
Pubmed
Dahlberg,
Nuclear translation: what is the evidence?
2003,
Pubmed
Dahlberg,
Functions of the GTPase Ran in RNA export from the nucleus.
1998,
Pubmed
Del Tito,
Effects of a minor isoleucyl tRNA on heterologous protein translation in Escherichia coli.
1995,
Pubmed
Dixon,
Persistence of nonribosome bound 5 S RNA in full-grown oocytes of Xenopus laevis.
1982,
Pubmed
,
Xenbase
Dunbar,
The U14 snoRNA is required for 2'-O-methylation of the pre-18S rRNA in Xenopus oocytes.
1998,
Pubmed
,
Xenbase
Dundr,
The roles of nucleolar structure and function in the subcellular location of the HIV-1 Rev protein.
1995,
Pubmed
Fatica,
Making ribosomes.
2002,
Pubmed
Fischer,
The HIV-1 Rev activation domain is a nuclear export signal that accesses an export pathway used by specific cellular RNAs.
1995,
Pubmed
Fornerod,
CRM1 is an export receptor for leucine-rich nuclear export signals.
1997,
Pubmed
,
Xenbase
Gadal,
Nuclear export of 60s ribosomal subunits depends on Xpo1p and requires a nuclear export sequence-containing factor, Nmd3p, that associates with the large subunit protein Rpl10p.
2001,
Pubmed
Görlich,
Transport between the cell nucleus and the cytoplasm.
1999,
Pubmed
Her,
Inhibition of Ran guanosine triphosphatase-dependent nuclear transport by the matrix protein of vesicular stomatitis virus.
1997,
Pubmed
,
Xenbase
Ho,
Nmd3p is a Crm1p-dependent adapter protein for nuclear export of the large ribosomal subunit.
2000,
Pubmed
Ho,
NMD3 encodes an essential cytoplasmic protein required for stable 60S ribosomal subunits in Saccharomyces cerevisiae.
1999,
Pubmed
Izaurralde,
The asymmetric distribution of the constituents of the Ran system is essential for transport into and out of the nucleus.
1997,
Pubmed
Izaurralde,
Transport of macromolecules between the nucleus and the cytoplasm.
1998,
Pubmed
Jarrous,
Human ribonuclease P: subunits, function, and intranuclear localization.
2002,
Pubmed
Johnson,
Nuclear export of ribosomal subunits.
2002,
Pubmed
Kaffman,
Regulation of nuclear localization: a key to a door.
1999,
Pubmed
Kudo,
Leptomycin B inhibition of signal-mediated nuclear export by direct binding to CRM1.
1998,
Pubmed
Leary,
Regulation of ribosome biogenesis within the nucleolus.
2001,
Pubmed
Lund,
Nonaqueous isolation of transcriptionally active nuclei from Xenopus oocytes.
1990,
Pubmed
,
Xenbase
Macara,
Transport into and out of the nucleus.
2001,
Pubmed
Mattaj,
Nucleocytoplasmic transport: the soluble phase.
1998,
Pubmed
Maxwell,
The small nucleolar RNAs.
1995,
Pubmed
Nakielny,
Transport of proteins and RNAs in and out of the nucleus.
1999,
Pubmed
Nissan,
60S pre-ribosome formation viewed from assembly in the nucleolus until export to the cytoplasm.
2002,
Pubmed
Olson,
The nucleolus: an old factory with unexpected capabilities.
2000,
Pubmed
Pasquinelli,
Reverse 5' caps in RNAs made in vitro by phage RNA polymerases.
1995,
Pubmed
,
Xenbase
Pasquinelli,
Inhibition of mRNA export in vertebrate cells by nuclear export signal conjugates.
1997,
Pubmed
,
Xenbase
Peculis,
Disruption of U8 nucleolar snRNA inhibits 5.8S and 28S rRNA processing in the Xenopus oocyte.
1993,
Pubmed
,
Xenbase
Petersen,
The matrix protein of vesicular stomatitis virus inhibits nucleocytoplasmic transport when it is in the nucleus and associated with nuclear pore complexes.
2000,
Pubmed
,
Xenbase
Robbins,
Two interdependent basic domains in nucleoplasmin nuclear targeting sequence: identification of a class of bipartite nuclear targeting sequence.
1991,
Pubmed
,
Xenbase
Savino,
In vivo disruption of Xenopus U3 snRNA affects ribosomal RNA processing.
1990,
Pubmed
,
Xenbase
Stade,
Exportin 1 (Crm1p) is an essential nuclear export factor.
1997,
Pubmed
Stage-Zimmermann,
Factors affecting nuclear export of the 60S ribosomal subunit in vivo.
2000,
Pubmed
Terns,
Nuclear transport of RNAs in microinjected Xenopus oocytes.
1998,
Pubmed
,
Xenbase
Thomas,
Biogenesis and nuclear export of ribosomal subunits in higher eukaryotes depend on the CRM1 export pathway.
2003,
Pubmed
,
Xenbase
Warner,
The nucleolus and ribosome formation.
1990,
Pubmed
Wen,
Identification of a signal for rapid export of proteins from the nucleus.
1995,
Pubmed
Wolff,
Leptomycin B is an inhibitor of nuclear export: inhibition of nucleo-cytoplasmic translocation of the human immunodeficiency virus type 1 (HIV-1) Rev protein and Rev-dependent mRNA.
1997,
Pubmed
Wong,
Subnuclear shuttling of human telomerase induced by transformation and DNA damage.
2002,
Pubmed
Wool,
Structure and evolution of mammalian ribosomal proteins.
1995,
Pubmed
Yang,
Nucleolar localization of hTERT protein is associated with telomerase function.
2002,
Pubmed
Zirwes,
A novel helicase-type protein in the nucleolus: protein NOH61.
2000,
Pubmed
,
Xenbase
van Eenennaam,
Basic domains target protein subunits of the RNase MRP complex to the nucleolus independently of complex association.
2001,
Pubmed
