Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Am J Physiol Cell Physiol
2016 Dec 01;3116:C884-C894. doi: 10.1152/ajpcell.00030.2016.
Show Gene links
Show Anatomy links
Functional and molecular identification of a TASK-1 potassium channel regulating chloride secretion through CFTR channels in the shark rectal gland: implications for cystic fibrosis.
Telles CJ
,
Decker SE
,
Motley WW
,
Peters AW
,
Mehr AP
,
Frizzell RA
,
Forrest JN
.
???displayArticle.abstract???
In the shark rectal gland (SRG), apical chloride secretion through CFTR channels is electrically coupled to a basolateral K+ conductance whose type and molecular identity are unknown. We performed studies in the perfused SRG with 17 K+ channel inhibitors to begin this search. Maximal chloride secretion was markedly inhibited by low-perfusate pH, bupivicaine, anandamide, zinc, quinidine, and quinine, consistent with the properties of an acid-sensitive, four-transmembrane, two-pore-domain K+ channel (4TM-K2P). Using PCR with degenerate primers to this family, we identified a TASK-1 fragment in shark rectal gland, brain, gill, and kidney. Using 5' and 3' rapid amplification of cDNA ends PCR and genomic walking, we cloned the full-length shark gene (1,282 bp), whose open reading frame encodes a protein of 375 amino acids that was 80% identical to the human TASK-1 protein. We expressed shark and human TASK-1 cRNA in Xenopus oocytes and characterized these channels using two-electrode voltage clamping. Both channels had identical current-voltage relationships (outward rectifying) and a reversal potential of -90 mV. Both were inhibited by quinine, bupivicaine, and acidic pH. The pKa for current inhibition was 7.75 for shark TASK-1 vs. 7.37 for human TASK-1, values similar to the arterial pH for each species. We identified this protein in SRG by Western blot and confocal immunofluorescent microscopy and detected the protein in SRG and human airway cells. Shark TASK-1 is the major K+ channel coupled to chloride secretion in the SRG, is the oldest 4TM 2P family member identified, and is the first TASK-1 channel identified to play a role in setting the driving force for chloride secretion in epithelia. The detection of this potassium channel in mammalian lung tissue has implications for human biology and disease.
Andersson,
Effects of inhibitors of small- and intermediate-conductance calcium-activated potassium channels, inwardly-rectifying potassium channels and Na(+)/K(+) ATPase on EDHF relaxations in the rat hepatic artery.
2000, Pubmed
Andersson,
Effects of inhibitors of small- and intermediate-conductance calcium-activated potassium channels, inwardly-rectifying potassium channels and Na(+)/K(+) ATPase on EDHF relaxations in the rat hepatic artery.
2000,
Pubmed
Balwierczak,
Evidence that BKCa channel activation contributes to K+ channel opener induced relaxation of the porcine coronary artery.
1995,
Pubmed
Bauer,
Electrophysiologic effects of the new I(Ks)-blocking agent chromanol 293b in the postinfarction canine heart. Preserved positive use-dependence and preferential prolongation of refractoriness in the infarct zone.
2000,
Pubmed
Beckett,
Functional and molecular identification of pH-sensitive K+ channels in murine urinary bladder smooth muscle.
2008,
Pubmed
Bewley,
Shark rectal gland vasoactive intestinal peptide receptor: cloning, functional expression, and regulation of CFTR chloride channels.
2006,
Pubmed
,
Xenbase
Bittner,
TASK1 modulates inflammation and neurodegeneration in autoimmune inflammation of the central nervous system.
2009,
Pubmed
Chen,
Protection of potassium channel inhibitors against hypoxia/reoxygenation-induced death of cultured hippocampal neurons.
2002,
Pubmed
Cho,
Inhibitory effect of tramadol on vasorelaxation mediated by ATP-sensitive K+ channels in rat aorta.
2007,
Pubmed
Cho,
A pH-sensitive potassium conductance (TASK) and its function in the murine gastrointestinal tract.
2005,
Pubmed
,
Xenbase
Clark,
Two-pore domain K⁺ channels regulate membrane potential of isolated human articular chondrocytes.
2011,
Pubmed
Cooper,
Characterization and function of TWIK-related acid sensing K+ channels in a rat nociceptive cell.
2004,
Pubmed
Davis,
Two-pore-domain potassium channels support anion secretion from human airway Calu-3 epithelial cells.
2006,
Pubmed
Doroshenko,
Acid-sensitive TASK-like K+ conductances contribute to resting membrane potential and to orexin-induced membrane depolarization in rat thalamic paraventricular nucleus neurons.
2009,
Pubmed
Du,
Human oocytes express ATP-sensitive K(+) channels.
2010,
Pubmed
Du,
Effects of chromanol 293B on transient outward and ultra-rapid delayed rectifier potassium currents in human atrial myocytes.
2003,
Pubmed
Duprat,
TASK, a human background K+ channel to sense external pH variations near physiological pH.
1997,
Pubmed
,
Xenbase
Forbush,
Na-K-Cl cotransport in the shark rectal gland. I. Regulation in the intact perfused gland.
1992,
Pubmed
Forbush,
The Na, K, C cotransporter of shark rectal gland.
1994,
Pubmed
Forrest,
Cellular and molecular biology of chloride secretion in the shark rectal gland: regulation by adenosine receptors.
1996,
Pubmed
Fujita,
Electrophysiological and pharmacological characterization of the K(ATP) channel involved in the K+-current responses to FSH and adenosine in the follicular cells of Xenopus oocyte.
2007,
Pubmed
,
Xenbase
Gray,
Volatile anesthetics activate the human tandem pore domain baseline K+ channel KCNK5.
2000,
Pubmed
,
Xenbase
Greger,
Mechanism of NaCl secretion in the rectal gland of spiny dogfish (Squalus acanthias). I. Experiments in isolated in vitro perfused rectal gland tubules.
1984,
Pubmed
Greger,
Mechanism of NaCl secretion in rectal gland tubules of spiny dogfish (Squalus acanthias). II. Effects of inhibitors.
1984,
Pubmed
Greger,
Potassium channels in the basolateral membrane of the rectal gland of the dogfish (Squalus acanthias).
1987,
Pubmed
Gögelein,
Potassium channels in the basolateral membrane of the rectal gland of Squalus acanthias. Regulation and inhibitors.
1987,
Pubmed
Higgins,
CLUSTAL V: improved software for multiple sequence alignment.
1992,
Pubmed
Illek,
Volume-sensitive basolateral K+ channels in HT-29/B6 cells: block by lidocaine, quinidine, NPPB, and Ba2+.
1992,
Pubmed
Johnson,
System-specific O2 sensitivity of the tandem pore domain K+ channel TASK-1.
2004,
Pubmed
Joiner,
Active K+ secretion through multiple KCa-type channels and regulation by IKCa channels in rat proximal colon.
2003,
Pubmed
Kelley,
Endogenous adenosine is an autacoid feedback inhibitor of chloride transport in the shark rectal gland.
1991,
Pubmed
Kerst,
Properties and function of KCNQ1 K+ channels isolated from the rectal gland of Squalus acanthias.
2001,
Pubmed
,
Xenbase
Kim,
TASK-3, a new member of the tandem pore K(+) channel family.
2000,
Pubmed
Kindler,
Amide local anesthetics potently inhibit the human tandem pore domain background K+ channel TASK-2 (KCNK5).
2003,
Pubmed
,
Xenbase
Lehrich,
Vasoactive intestinal peptide, forskolin, and genistein increase apical CFTR trafficking in the rectal gland of the spiny dogfish, Squalus acanthias. Acute regulation of CFTR trafficking in an intact epithelium.
1998,
Pubmed
,
Xenbase
Leonoudakis,
An open rectifier potassium channel with two pore domains in tandem cloned from rat cerebellum.
1998,
Pubmed
,
Xenbase
Lesage,
Molecular and functional properties of two-pore-domain potassium channels.
2000,
Pubmed
Levy,
Segment-specific expression of 2P domain potassium channel genes in human nephron.
2004,
Pubmed
Lima,
Role of ATP-sensitive potassium channels in normal and hypertension-associated pregnancy in rats.
2006,
Pubmed
Limberg,
TASK-1 channels may modulate action potential duration of human atrial cardiomyocytes.
2011,
Pubmed
,
Xenbase
Lin,
The effect of high extracellular potassium on IKr inhibition by anti-arrhythmic agents.
2007,
Pubmed
,
Xenbase
Loganathan,
Basolateral potassium (IKCa) channel inhibition prevents increased colonic permeability induced by chemical hypoxia.
2011,
Pubmed
Lotshaw,
Biophysical and pharmacological characteristics of native two-pore domain TASK channels in rat adrenal glomerulosa cells.
2006,
Pubmed
Maingret,
The endocannabinoid anandamide is a direct and selective blocker of the background K(+) channel TASK-1.
2001,
Pubmed
Medhurst,
Distribution analysis of human two pore domain potassium channels in tissues of the central nervous system and periphery.
2001,
Pubmed
Melin,
CFTR inhibition by glibenclamide requires a positive charge in cytoplasmic loop three.
2007,
Pubmed
Olschewski,
Impact of TASK-1 in human pulmonary artery smooth muscle cells.
2006,
Pubmed
Prior,
Functions of large conductance Ca2+-activated (BKCa), delayed rectifier (KV) and background K+ channels in the control of membrane potential in rabbit renal arcuate artery.
1998,
Pubmed
Reyes,
Cloning and expression of a novel pH-sensitive two pore domain K+ channel from human kidney.
1998,
Pubmed
Richter,
Acid-sensitive two-pore domain potassium (K2P) channels in mouse taste buds.
2004,
Pubmed
Rose,
Consensus-degenerate hybrid oligonucleotide primers for amplification of distantly related sequences.
1998,
Pubmed
Schultz,
Glibenclamide blockade of CFTR chloride channels.
1996,
Pubmed
Silva,
Mechanism of active chloride secretion by shark rectal gland: role of Na-K-ATPase in chloride transport.
1977,
Pubmed
Silva,
Indirect evidence for enhancement of Na-K-ATPase activity with stimulation of rectal gland secretion.
1979,
Pubmed
Silva,
Inhibition of chloride secretion by BaCl2 in the rectal gland of the spiny dogfish, Squalus acanthias.
1986,
Pubmed
Sobko,
Heteromultimeric delayed-rectifier K+ channels in schwann cells: developmental expression and role in cell proliferation.
1998,
Pubmed
Stahl,
Divergent CFTR orthologs respond differently to the channel inhibitors CFTRinh-172, glibenclamide, and GlyH-101.
2012,
Pubmed
,
Xenbase
Szewczyk,
Effects of inhibitors and activators of ATP-regulated K+ channel on mitochondrial potassium uniport.
1996,
Pubmed
Tang,
[Influence of KATP channel inhibitor on the changes of HSP70 expression in sevoflurane-induced neonatal rat cardiomyocytes].
2003,
Pubmed
Tang,
Block of mouse Slo1 and Slo3 K+ channels by CTX, IbTX, TEA, 4-AP and quinidine.
2010,
Pubmed
,
Xenbase
Thompson,
CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice.
1994,
Pubmed
Varro,
The role of the delayed rectifier component IKs in dog ventricular muscle and Purkinje fibre repolarization.
2000,
Pubmed
Waldegger,
Molecular and functional characterization of s-KCNQ1 potassium channel from rectal gland of Squalus acanthias.
1999,
Pubmed
,
Xenbase
Xu,
Molecular cloning and functional expression of the bumetanide-sensitive Na-K-Cl cotransporter.
1994,
Pubmed
Xu,
Kaempferol stimulates large conductance Ca2+ -activated K+ (BKCa) channels in human umbilical vein endothelial cells via a cAMP/PKA-dependent pathway.
2008,
Pubmed
Yanagisawa,
Interaction of potassium channel openers and blockers in canine atrial muscle.
1989,
Pubmed
Zygmunt,
Effects of potassium channel inhibitors on nitrergic and adrenergic neurotransmission in lamina propria of the female rabbit urethra.
1996,
Pubmed