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Proteomics
2016 Dec 01;1623:2945-2952. doi: 10.1002/pmic.201600262.
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Over 4100 protein identifications from a Xenopus laevis fertilized egg digest using reversed-phase chromatographic prefractionation followed by capillary zone electrophoresis-electrospray ionization-tandem mass spectrometry analysis.
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A tryptic digest generated from Xenopus laevis fertilized embryos was fractionated by RPLC. One set of 30 fractions was analyzed by 100-min CZE-ESI-MS/MS separations (50 h total instrument time), and a second set of 15 fractions was analyzed by 3-h UPLC-ESI-MS/MS separations (45 h total instrument time). CZE-MS/MS produced 70% as many protein IDs (4134 versus 5787) and 60% as many peptide IDs (22 535 versus 36 848) as UPLC-MS/MS with similar instrument time (50 h versus 45 h) but with 50 times smaller total consumed sample amount (1.5 μg versus 75 μg). Surprisingly, CZE generated peaks that were 25% more intense than UPLC for peptides that were identified by both techniques, despite the 50-fold lower loading amount; this high sensitivity reflects the efficient ionization produced by the electrokinetically pumped nanospray interface used in CZE. This report is the first comparison of CZE-MS/MS and UPLC-MS/MS for large-scale eukaryotic proteomic analysis. The numbers of protein and peptide identifications produced by CZE-ESI-MS/MS approach those produced by UPLC-MS/MS, but with nearly two orders of magnitude lower sample amounts.
Aebersold,
Mass spectrometry-based proteomics.
2003, Pubmed
Aebersold,
Mass spectrometry-based proteomics.
2003,
Pubmed
Aebersold,
Mass spectrometry in proteomics.
2001,
Pubmed
Chait,
Chemistry. Mass spectrometry: bottom-up or top-down?
2006,
Pubmed
Cox,
Andromeda: a peptide search engine integrated into the MaxQuant environment.
2011,
Pubmed
Faserl,
Quantitative proteomics using ultralow flow capillary electrophoresis-mass spectrometry.
2015,
Pubmed
Gygi,
Quantitative analysis of complex protein mixtures using isotope-coded affinity tags.
1999,
Pubmed
Iwasaki,
One-dimensional capillary liquid chromatographic separation coupled with tandem mass spectrometry unveils the Escherichia coli proteome on a microarray scale.
2010,
Pubmed
Lee,
Staggered multistep elution solid-phase extraction capillary electrophoresis/tandem mass spectrometry: a high-throughput approach in protein analysis.
2011,
Pubmed
Li,
A replaceable microreactor for on-line protein digestion in a two-dimensional capillary electrophoresis system with tandem mass spectrometry detection.
2011,
Pubmed
Mann,
The coming age of complete, accurate, and ubiquitous proteomes.
2013,
Pubmed
Maxwell,
Decoupling CE and ESI for a more robust interface with MS.
2010,
Pubmed
Maxwell,
Twenty years of interface development for capillary electrophoresis-electrospray ionization-mass spectrometry.
2008,
Pubmed
Moini,
Simplifying CE-MS operation. 2. Interfacing low-flow separation techniques to mass spectrometry using a porous tip.
2007,
Pubmed
Peuchen,
Optimization and comparison of bottom-up proteomic sample preparation for early-stage Xenopus laevis embryos.
2016,
Pubmed
,
Xenbase
Plumb,
Application of ultra performance liquid chromatography-mass spectrometry to profiling rat and dog bile.
2009,
Pubmed
Pröfrock,
Progress and possible applications of miniaturised separation techniques and elemental mass spectrometry for quantitative, heteroatom-tagged proteomics.
2010,
Pubmed
Shihabi,
Stacking in capillary zone electrophoresis.
2000,
Pubmed
Song,
Reversed-phase-reversed-phase liquid chromatography approach with high orthogonality for multidimensional separation of phosphopeptides.
2010,
Pubmed
Sun,
Third-generation electrokinetically pumped sheath-flow nanospray interface with improved stability and sensitivity for automated capillary zone electrophoresis-mass spectrometry analysis of complex proteome digests.
2015,
Pubmed
,
Xenbase
Sun,
Over 10,000 peptide identifications from the HeLa proteome by using single-shot capillary zone electrophoresis combined with tandem mass spectrometry.
2014,
Pubmed
Tong,
Identification of proteins in complexes by solid-phase microextraction/multistep elution/capillary electrophoresis/tandem mass spectrometry.
1999,
Pubmed
Wang,
Improving the comprehensiveness and sensitivity of sheathless capillary electrophoresis-tandem mass spectrometry for proteomic analysis.
2012,
Pubmed
Wojcik,
Simplified capillary electrophoresis nanospray sheath-flow interface for high efficiency and sensitive peptide analysis.
2010,
Pubmed
Wühr,
Deep proteomics of the Xenopus laevis egg using an mRNA-derived reference database.
2014,
Pubmed
,
Xenbase
Zhang,
Protein analysis by shotgun/bottom-up proteomics.
2013,
Pubmed
Zhu,
Single-shot proteomics using capillary zone electrophoresis-electrospray ionization-tandem mass spectrometry with production of more than 1250 Escherichia coli peptide identifications in a 50 min separation.
2013,
Pubmed
Zhu,
Stable, reproducible, and automated capillary zone electrophoresis-tandem mass spectrometry system with an electrokinetically pumped sheath-flow nanospray interface.
2014,
Pubmed
Zhu,
Bottom-up proteomics of Escherichia coli using dynamic pH junction preconcentration and capillary zone electrophoresis-electrospray ionization-tandem mass spectrometry.
2014,
Pubmed
Zhu,
Thermally-initiated free radical polymerization for reproducible production of stable linear polyacrylamide coated capillaries, and their application to proteomic analysis using capillary zone electrophoresis-mass spectrometry.
2016,
Pubmed
