Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
???displayArticle.abstract???
Poly(A)+RNA and tubulin mRNA are localized in the periphery of Xenopus oocytes and become delocalized during meiotic maturation. Delocalization of this RNA can be triggered by incubation in agents which reduce entry of calcium ions into the cell (e.g. lanthanum chloride and verapamil). Although these agents ordinarily promote meiotic maturation, addition of theophylline to the medium will inhibit maturation but not delocalization. Manipulations which prevent calcium entry without inducing meiotic maturation (e.g. calcium-free buffer) are also shown to trigger disruption of the RNA localization. In addition, manipulations which reduce chloride efflux from the cell (e.g. increasing the external chloride ion concentration with choline chloride) result in disruption of the localization of poly (A)+ RNA and tubulin mRNA without inducing meiotic maturation. The calcium-dependent chloride efflux present in Xenopus oocytes disappears after the oocyte has been stimulated to proceed through meiotic maturation. We show that reduction of the influx of calcium ions or efflux of chloride ions induces the delocalization of poly (A)+RNA and tubulin mRNA without inducing meiotic maturation. We suggest, therefore, that reducing the transmembrane movement of these ions is likely to be the natural trigger for the delocalization of poly(A)+RNA and tubulin mRNA.
Barish,
A transient calcium-dependent chloride current in the immature Xenopus oocyte.
1983, Pubmed,
Xenbase
Barish,
A transient calcium-dependent chloride current in the immature Xenopus oocyte.
1983,
Pubmed
,
Xenbase
BEERS,
Hydrolysis of polyadenylic acid by pancreatic ribonuclease.
1960,
Pubmed
Borgens,
Bioelectricity and regeneration: large currents leave the stumps of regenerating newt limbs.
1977,
Pubmed
Capco,
Transient localizations of messenger RNA in Xenopus laevis oocytes.
1982,
Pubmed
,
Xenbase
Capco,
Localized protein synthesis during oogenesis of Xenopus laevis: analysis by in situ translation.
1982,
Pubmed
,
Xenbase
Capco,
The spatial pattern of RNA in fully grown oocytes of an amphibian, Xenopus laevis.
1982,
Pubmed
,
Xenbase
Capco,
Analysis of proteins in the peripheral and central regions of amphibian oocytes and eggs.
1988,
Pubmed
,
Xenbase
Capco,
Cytoskeletal reorganization during early mammalian development: analysis using embedment-free sections.
1986,
Pubmed
Charbonneau,
The onset of activation responsiveness during maturation coincides with the formation of the cortical endoplasmic reticulum in oocytes of Xenopus laevis.
1984,
Pubmed
,
Xenbase
Craig,
Regulation of actin polymerization by villin, a 95,000 dalton cytoskeletal component of intestinal brush borders.
1980,
Pubmed
Darnell,
An adenylic acid-rich sequence in messenger RNA of HeLa cells and its possible relationship to reiterated sites in DNA.
1971,
Pubmed
Dworkin,
Changes in RNA titers and polyadenylation during oogenesis and oocyte maturation in Xenopus laevis.
1985,
Pubmed
,
Xenbase
Fleckenstein,
Specific pharmacology of calcium in myocardium, cardiac pacemakers, and vascular smooth muscle.
1977,
Pubmed
Glenney,
The spectrin-related molecule, TW-260/240, cross-links the actin bundles of the microvillus rootlets in the brush borders of intestinal epithelial cells.
1983,
Pubmed
Godsave,
Oocytes and early embryos of Xenopus laevis contain intermediate filaments which react with anti-mammalian vimentin antibodies.
1984,
Pubmed
,
Xenbase
Jaffe,
Electrophoresis along cell membranes.
1977,
Pubmed
Jaffe,
Neurites grow faster towards the cathode than the anode in a steady field.
1979,
Pubmed
Jaffe,
Strong electrical currents leave the primitive streak of chick embryos.
1979,
Pubmed
Jeffery,
Messenger RNA in the cytoskeletal framework: analysis by in situ hybridization.
1982,
Pubmed
Jeffery,
The spatial distribution of maternal mRNA is determined by a cortical cytoskeletal domain in Chaetopterus eggs.
1985,
Pubmed
Langer,
Lanthanum in heart cell culture. Effect on calcium exchange correlated with its localization.
1972,
Pubmed
Larabell,
Freeze-fracture analysis of structural reorganization during meiotic maturation in oocytes of Xenopus laevis.
1988,
Pubmed
,
Xenbase
Lenk,
A cytoskeletal structure with associated polyribosomes obtained from HeLa cells.
1977,
Pubmed
Maller,
Early effect of progesterone on levels of cyclic adenosine 3':5'-monophosphate in Xenopus oocytes.
1979,
Pubmed
,
Xenbase
Miledi,
Lanthanum ions abolish the "calcium response" of nerve terminals.
1971,
Pubmed
Mooseker,
Actin binding proteins of the brush border.
1983,
Pubmed
Moreau,
Free calcium changes associated with hormone action in amphibian oocytes.
1980,
Pubmed
,
Xenbase
Nachshen,
The effects of some organic "calcium antagonists" on calcium influx in presynaptic nerve terminals.
1979,
Pubmed
Nuccitelli,
Polarity of isolated blastomeres from mouse morulae: detection of transcellular ion currents.
1985,
Pubmed
O'Connor,
Calcium, potassium, and sodium exchange by full-grown and maturing Xenopus laevis oocytes.
1977,
Pubmed
,
Xenbase
O'Connor,
Inhibition of oocyte maturation by theophylline: possible mechanism of action.
1976,
Pubmed
,
Xenbase
Phillips,
Spatial changes in poly(A) concentrations during early embryogenesis in Xenopus laevis: analysis by in situ hybridization.
1985,
Pubmed
,
Xenbase
Pinto,
The different effects of D-600 (methoxyverapamil) on the release of adrenal catecholamines induced by acetylcholine, high potassium or sodium deprivation.
1976,
Pubmed
Poo,
In situ electrophoresis of membrane components.
1981,
Pubmed
,
Xenbase
Ritchie,
Catecholamine secretion in a rat pheochromocytoma cell line: two pathways for calcium entry.
1979,
Pubmed
Robinson,
Electrical currents through full-grown and maturing Xenopus oocytes.
1979,
Pubmed
,
Xenbase
Schorderet-Slatkine,
Progesterone-induced meiotic reinitation in vitro in Xenopus laevis oocytes: a role for the displacement of membrane-bound calcium.
1977,
Pubmed
,
Xenbase
Schorderet-Slatkine,
Initiation of meiotic maturation in Xenopus laevis oocytes by lanthanum.
1976,
Pubmed
,
Xenbase
Steinhardt,
Intracellular calcium release at fertilization in the sea urchin egg.
1977,
Pubmed
Wallace,
Maturation of Xenopus oocytes. II. Observations on membrane potential.
1977,
Pubmed
,
Xenbase
Wasserman,
Progesterone induces a rapid increase in [Ca2+]in of Xenopus laevis oocytes.
1980,
Pubmed
,
Xenbase
Weinberger,
Locomotion and adhesion of amphibian gastrula and neurula cells cultured on substrata of varied surface charge.
1980,
Pubmed
Woodruff,
Electrophoresis of proteins in intercellular bridges.
1980,
Pubmed
Woodruff,
Polarized intercellular bridges in ovarian follicles of the cecropia moth.
1973,
Pubmed
Wylie,
The cytoskeleton of Xenopus oocytes and its role in development.
1985,
Pubmed
,
Xenbase
