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Wilehm Roux Arch Dev Biol
1977 Dec 01;1824:347-360. doi: 10.1007/BF00848385.
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Factors promoting the establishment of primary cultures of liver cells fromXenopus larvae.
Wahli W
,
Weber R
.
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The requirements for establishment and survival of primary cultures of larval amphibian liver cells were investigated.Plating efficiency was found to be enhanced by a collagen substrate, by diluted conditioned medium from an adultXenopus kidney cell line and by high initial cell densities. Plating efficiency was highest at a tonicity of 165-220 mOsm/kg. In cultures with undiluted conditioned medium the increase in cell number was 50-60% greater than in controls, where it was about 2-fold between day 3 and 6 of culture. Conditioned medium from theXenopus kidney cell line is assumed to contain at least two components, which are effective at different concentrations and stimulate either plating efficiency and cell aggregation or cell proliferation.In cultures without collagen sheets, cell flattening is greatly reduced, indicating that cell shape is also dependent upon the substrate.
Arthur,
Amphibian cells in vitro. I. Growth of Xenopus cells in a soft agar medium and on an agar surface.
1971, Pubmed,
Xenbase
Arthur,
Amphibian cells in vitro. I. Growth of Xenopus cells in a soft agar medium and on an agar surface.
1971,
Pubmed
,
Xenbase
Balinsky,
The effect of thyroxine and hypertonic environment on the enzymes of the urea cycle in Xenopus laevis.
1972,
Pubmed
,
Xenbase
Balls,
Amphibian cells in vitro. II. Effects of variations in medium osmolarity on a permanent cells line isolated from Xenopus.
1973,
Pubmed
,
Xenbase
Balsamo,
Embryonic cell aggregation: kinetics and specificity of binding of enhancing factors.
1974,
Pubmed
Bennett,
Thyroxine stimulation of ornithine transcarbamylase activity and protein synthesis in tadpole (RNA catesbeiana) liver in organ culture.
1969,
Pubmed
Blatt,
The effect of thyroxine on ribonucleic acid synthesis by premetamorphic tadpole liver cell suspensions.
1969,
Pubmed
Calderon,
Two biological activities regulating cell proliferation found in cultures of peritoneal exudate cells.
1975,
Pubmed
Chen,
Patterns and metamorphic changes of serum proteins in amphibia.
1970,
Pubmed
Dulak,
A partially purified polypeptide fraction from rat liver cell conditioned medium with multiplication-stimulating activity for embryo fibroblasts.
1973,
Pubmed
Garber,
Reconstruction of brain tissue from cell suspensions. 2. Specific enhancement of aggregation of embryonic cerebral cells by supernatant from homologous cell cultures.
1972,
Pubmed
GASCHE,
[On the question of the point of attack of Thiouracil; Trials on Xenopus larvae].
1946,
Pubmed
,
Xenbase
Godsell,
The cell cycle of Xenopus laevis cells in monolayer culture.
1974,
Pubmed
,
Xenbase
Kistler,
Enzyme patterns in mitochondria of eggs, liver, and skeletal muscle during larval development of Xenopus.
1974,
Pubmed
,
Xenbase
Koch,
Growth control of differentiated fetal rat hepatocytes in primary monolayer culture. VI. Studies with conditioned medium and its functional interactions with serum factors.
1974,
Pubmed
Ledford,
Albumin synthesis during induced and spontaneous metamorphosis in the bullfrog Rana catesbeiana.
1973,
Pubmed
Leffert,
Studies on primary cultures of differentiated fetal liver cells.
1972,
Pubmed
McGuire,
Intercellular adhesive selectivity. II. Properties of embryonic chick liver cell-cell adhesion.
1976,
Pubmed
Michalopoulos,
Primary culture of parenchymal liver cells on collagen membranes. Morphological and biochemical observations.
1975,
Pubmed
Monnickendam,
Amphibian organ culture.
1973,
Pubmed
,
Xenbase
Moscona,
Aggregation of embryonic cells in a serum-free medium and its inhibition at suboptimal temperatures.
1966,
Pubmed
Nagano,
Increase in serum albumin during bullfrog metamorphosis.
1973,
Pubmed
SHAFFER,
The isolated Xenopus laevis tail: a preparation for studying the central nervous system and metamorphosis in culture.
1963,
Pubmed
,
Xenbase
Shambaugh,
Synthesis of carbamyl phosphate synthetase in amphibian liver in vitro. The effect of thyroxine.
1969,
Pubmed
Solursh,
Long-term cell culture of two differentiated cell types from the liver of larval and adult Xenopus laevis.
1972,
Pubmed
,
Xenbase
Takahashi,
An analysis of the effect of "conditioned medium" upon the cell culture at low density.
1970,
Pubmed
Takahashi,
Separation of two factors affecting the aggregation kinetics from the conditioned medium.
1971,
Pubmed
Tata,
Requirement for RNA and protein synthesis for induced regression of the tadpole tail in organ culture.
1966,
Pubmed
Watanabe,
Changes in the capacity for clonal growth and differentiation in vitro of the vertebral cartilage cells with embryonic development. II. Vitalizing effect of conditioned medium on the cells of younger embryos.
1971,
Pubmed
WOOD,
The formation of fibrils from collagen solutions. 1. The effect of experimental conditions: kinetic and electron-microscope studies.
1960,
Pubmed
