Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Proc Natl Acad Sci U S A
2020 Aug 18;11733:20298-20304. doi: 10.1073/pnas.2004303117.
Show Gene links
Show Anatomy links
A folding reaction at the C-terminal domain drives temperature sensing in TRPM8 channels.
Díaz-Franulic I
,
Raddatz N
,
Castillo K
,
González-Nilo FD
,
Latorre R
.
???displayArticle.abstract???
In mammals, temperature-sensitive TRP channels make membrane conductance of cells extremely temperature dependent, allowing the detection of temperature ranging from noxious cold to noxious heat. We progressively deleted the distal carboxyl terminus domain (CTD) of the cold-activated melastatin receptor channel, TRPM8. We found that the enthalpy change associated with channel gating is proportional to the length of the CTD. Deletion of the last 36 amino acids of the CTD transforms TRPM8 into a reduced temperature-sensitivity channel (Q10 ∼4). Exposing the intracellular domain to a denaturing agent increases the energy required to open the channel indicating that cold drives channel gating by stabilizing the folded state of the CTD. Experiments in the presence of an osmoticant agent suggest that channel gating involves a change in solute-inaccessible volume in the CTD of ∼1,900 Å3 This volume matches the void space inside the coiled coil according to the cryogenic electron microscopy structure of TRPM8. The results indicate that a folding-unfolding reaction of a specialized temperature-sensitive structure is coupled to TRPM8 gating.
Ando,
Structural and thermodynamic characterization of T4 lysozyme mutants and the contribution of internal cavities to pressure denaturation.
2008, Pubmed
Ando,
Structural and thermodynamic characterization of T4 lysozyme mutants and the contribution of internal cavities to pressure denaturation.
2008,
Pubmed
Arrigoni,
Unfolding of a Temperature-Sensitive Domain Controls Voltage-Gated Channel Activation.
2016,
Pubmed
Arrigoni,
Global versus local mechanisms of temperature sensing in ion channels.
2018,
Pubmed
Bothe,
The Complex Energy Landscape of the Protein IscU.
2015,
Pubmed
Brauchi,
A hot-sensing cold receptor: C-terminal domain determines thermosensation in transient receptor potential channels.
2006,
Pubmed
Brauchi,
Clues to understanding cold sensation: thermodynamics and electrophysiological analysis of the cold receptor TRPM8.
2004,
Pubmed
Cao,
TRPV1 structures in distinct conformations reveal activation mechanisms.
2013,
Pubmed
Chao,
Kinetic study on the formation of a de novo designed heterodimeric coiled-coil: use of surface plasmon resonance to monitor the association and dissociation of polypeptide chains.
1996,
Pubmed
Clapham,
The TRP ion channel family.
2001,
Pubmed
Clapham,
A thermodynamic framework for understanding temperature sensing by transient receptor potential (TRP) channels.
2011,
Pubmed
Collins,
Cooperative water filling of a nonpolar protein cavity observed by high-pressure crystallography and simulation.
2005,
Pubmed
Cordero-Morales,
Cytoplasmic ankyrin repeats of transient receptor potential A1 (TRPA1) dictate sensitivity to thermal and chemical stimuli.
2011,
Pubmed
,
Xenbase
Correa,
Gating kinetics of batrachotoxin-modified Na+ channels in the squid giant axon. Voltage and temperature effects.
1992,
Pubmed
CRICK,
Is alpha-keratin a coiled coil?
1952,
Pubmed
De Crescenzo,
Real-time monitoring of the interactions of two-stranded de novo designed coiled-coils: effect of chain length on the kinetic and thermodynamic constants of binding.
2003,
Pubmed
Diaz-Franulic,
Allosterism and Structure in Thermally Activated Transient Receptor Potential Channels.
2016,
Pubmed
Díaz-Franulic,
Gating-induced large aqueous volumetric remodeling and aspartate tolerance in the voltage sensor domain of Shaker K+ channels.
2018,
Pubmed
,
Xenbase
Diver,
Structural insights into TRPM8 inhibition and desensitization.
2019,
Pubmed
Emir,
Molecular Mechanisms of Temperature Gating in TRP Channels
2017,
Pubmed
Erler,
Trafficking and assembly of the cold-sensitive TRPM8 channel.
2006,
Pubmed
Feng,
Temperature sensing by thermal TRP channels: thermodynamic basis and molecular insights.
2014,
Pubmed
Frye,
Probing the contribution of internal cavities to the volume change of protein unfolding under pressure.
1998,
Pubmed
Fujiwara,
Temperature-sensitive gating of voltage-gated proton channels.
2014,
Pubmed
Fujiwara,
The cytoplasmic coiled-coil mediates cooperative gating temperature sensitivity in the voltage-gated H(+) channel Hv1.
2012,
Pubmed
Gal-Mor,
The temperature-sensing protein TlpA is repressed by PhoP and dispensable for virulence of Salmonella enterica serovar Typhimurium in mice.
2006,
Pubmed
García-Ávila,
What is new about mild temperature sensing? A review of recent findings.
2019,
Pubmed
Gracheva,
Ganglion-specific splicing of TRPV1 underlies infrared sensation in vampire bats.
2011,
Pubmed
Gregorio-Teruel,
Mutation of I696 and W697 in the TRP box of vanilloid receptor subtype I modulates allosteric channel activation.
2014,
Pubmed
Huang,
A structural overview of the ion channels of the TRPM family.
2020,
Pubmed
Humphrey,
VMD: visual molecular dynamics.
1996,
Pubmed
Jabba,
Directionality of temperature activation in mouse TRPA1 ion channel can be inverted by single-point mutations in ankyrin repeat six.
2014,
Pubmed
Jara-Oseguera,
The role of allosteric coupling on thermal activation of thermo-TRP channels.
2013,
Pubmed
Jiang,
C-type inactivation involves a significant decrease in the intracellular aqueous pore volume of Kv1.4 K+ channels expressed in Xenopus oocytes.
2003,
Pubmed
,
Xenbase
Kukita,
Solvent effects on squid sodium channels are attributable to movements of a flexible protein structure in gating currents and to hydration in a pore.
2000,
Pubmed
Labro,
Inverted allosteric coupling between activation and inactivation gates in K+ channels.
2018,
Pubmed
Latorre,
Molecular coupling between voltage sensor and pore opening in the Arabidopsis inward rectifier K+ channel KAT1.
2003,
Pubmed
Litowski,
Designing heterodimeric two-stranded alpha-helical coiled-coils. Effects of hydrophobicity and alpha-helical propensity on protein folding, stability, and specificity.
2002,
Pubmed
Liu,
TRPM8 in health and disease: cold sensing and beyond.
2011,
Pubmed
Liu,
Functional control of cold- and menthol-sensitive TRPM8 ion channels by phosphatidylinositol 4,5-bisphosphate.
2005,
Pubmed
,
Xenbase
Lupas,
The structure of alpha-helical coiled coils.
2005,
Pubmed
Makhatadze,
Heat capacity of proteins. I. Partial molar heat capacity of individual amino acid residues in aqueous solution: hydration effect.
1990,
Pubmed
Männikkö,
Voltage-sensing mechanism is conserved among ion channels gated by opposite voltages.
2002,
Pubmed
,
Xenbase
Matta,
Voltage is a partial activator of rat thermosensitive TRP channels.
2007,
Pubmed
McGoldrick,
Structure of the thermo-sensitive TRP channel TRP1 from the alga Chlamydomonas reinhardtii.
2019,
Pubmed
Moparthi,
Human TRPA1 is a heat sensor displaying intrinsic U-shaped thermosensitivity.
2016,
Pubmed
Nilius,
Gating of TRP channels: a voltage connection?
2005,
Pubmed
Pastore,
Unbiased cold denaturation: low- and high-temperature unfolding of yeast frataxin under physiological conditions.
2007,
Pubmed
Paulsen,
Structure of the TRPA1 ion channel suggests regulatory mechanisms.
2015,
Pubmed
Peier,
A TRP channel that senses cold stimuli and menthol.
2002,
Pubmed
Piraner,
Modular Thermal Control of Protein Dimerization.
2019,
Pubmed
Privalov,
Cold denaturation of proteins.
1990,
Pubmed
Privalov,
Stability of proteins: small globular proteins.
1979,
Pubmed
Qin,
Demystifying thermal channels: driving a channel both forwards and backwards with a single gear?
2013,
Pubmed
Raddatz,
Temperature and voltage coupling to channel opening in transient receptor potential melastatin 8 (TRPM8).
2014,
Pubmed
,
Xenbase
Rohács,
PI(4,5)P2 regulates the activation and desensitization of TRPM8 channels through the TRP domain.
2005,
Pubmed
Saita,
A coiled coil switch mediates cold sensing by the thermosensory protein DesK.
2015,
Pubmed
Singh,
Structural basis of temperature sensation by the TRP channel TRPV3.
2019,
Pubmed
Singh,
Structure and gating mechanism of the transient receptor potential channel TRPV3.
2018,
Pubmed
Voets,
Sensing with TRP channels.
2005,
Pubmed
Voets,
The principle of temperature-dependent gating in cold- and heat-sensitive TRP channels.
2004,
Pubmed
Wang,
Structures and gating mechanism of human TRPM2.
2018,
Pubmed
Yin,
Structure of the cold- and menthol-sensing ion channel TRPM8.
2018,
Pubmed
Zimmerberg,
Solute inaccessible aqueous volume changes during opening of the potassium channel of the squid giant axon.
1990,
Pubmed
Zimmerberg,
Polymer inaccessible volume changes during opening and closing of a voltage-dependent ionic channel.
,
Pubmed
Zimmerberg,
Water movement during channel opening and closing.
1987,
Pubmed
Zubcevic,
Cryo-electron microscopy structure of the TRPV2 ion channel.
2016,
Pubmed
