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SPIN90 associates with mDia1 and the Arp2/3 complex to regulate cortical actin organization.
Cao L
,
Yonis A
,
Vaghela M
,
Barriga EH
,
Chugh P
,
Smith MB
,
Maufront J
,
Lavoie G
,
Méant A
,
Ferber E
,
Bovellan M
,
Alberts A
,
Bertin A
,
Mayor R
,
Paluch EK
,
Roux PP
,
Jégou A
,
Romet-Lemonne G
,
Charras G
.
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Cell shape is controlled by the submembranous cortex, an actomyosin network mainly generated by two actin nucleators: the Arp2/3 complex and the formin mDia1. Changes in relative nucleator activity may alter cortical organization, mechanics and cell shape. Here we investigate how nucleation-promoting factors mediate interactions between nucleators. In vitro, the nucleation-promoting factor SPIN90 promotes formation of unbranched filaments by Arp2/3, a process thought to provide the initial filament for generation of dendritic networks. Paradoxically, in cells, SPIN90 appears to favour a formin-dominated cortex. Our in vitro experiments reveal that this feature stems mainly from two mechanisms: efficient recruitment of mDia1 to SPIN90-Arp2/3 nucleated filaments and formation of a ternary SPIN90-Arp2/3-mDia1 complex that greatly enhances filament nucleation. Both mechanisms yield rapidly elongating filaments with mDia1 at their barbed ends and SPIN90-Arp2/3 at their pointed ends. Thus, in networks, SPIN90 lowers branching densities and increases the proportion of long filaments elongated by mDia1.
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