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Proc Natl Acad Sci U S A
2021 Jan 05;1181:. doi: 10.1073/pnas.2020857118.
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Naphthylphthalamic acid associates with and inhibits PIN auxin transporters.
Abas L
,
Kolb M
,
Stadlmann J
,
Janacek DP
,
Lukic K
,
Schwechheimer C
,
Sazanov LA
,
Mach L
,
Friml J
,
Hammes UZ
.
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N-1-naphthylphthalamic acid (NPA) is a key inhibitor of directional (polar) transport of the hormone auxin in plants. For decades, it has been a pivotal tool in elucidating the unique polar auxin transport-based processes underlying plant growth and development. Its exact mode of action has long been sought after and is still being debated, with prevailing mechanistic schemes describing only indirect connections between NPA and the main transporters responsible for directional transport, namely PIN auxin exporters. Here we present data supporting a model in which NPA associates with PINs in a more direct manner than hitherto postulated. We show that NPA inhibits PIN activity in a heterologous oocyte system and that expression of NPA-sensitive PINs in plant, yeast, and oocyte membranes leads to specific saturable NPA binding. We thus propose that PINs are a bona fide NPA target. This offers a straightforward molecular basis for NPA inhibition of PIN-dependent auxin transport and a logical parsimonious explanation for the known physiological effects of NPA on plant growth, as well as an alternative hypothesis to interpret past and future results. We also introduce PIN dimerization and describe an effect of NPA on this, suggesting that NPA binding could be exploited to gain insights into structural aspects of PINs related to their transport mechanism.
Abas,
Maximum yields of microsomal-type membranes from small amounts of plant material without requiring ultracentrifugation.
2010, Pubmed
Abas,
Maximum yields of microsomal-type membranes from small amounts of plant material without requiring ultracentrifugation.
2010,
Pubmed
Adamowski,
PIN-dependent auxin transport: action, regulation, and evolution.
2015,
Pubmed
Bailly,
Modulation of P-glycoproteins by auxin transport inhibitors is mediated by interaction with immunophilins.
2008,
Pubmed
Benková,
Local, efflux-dependent auxin gradients as a common module for plant organ formation.
2003,
Pubmed
Bergeron,
Frog oocytes to unveil the structure and supramolecular organization of human transport proteins.
2011,
Pubmed
,
Xenbase
Bernasconi,
The N-1-Naphthylphthalamic Acid-Binding Protein Is an Integral Membrane Protein.
1996,
Pubmed
Blakeslee,
Interactions among PIN-FORMED and P-glycoprotein auxin transporters in Arabidopsis.
2007,
Pubmed
Brown,
Flavonoids act as negative regulators of auxin transport in vivo in arabidopsis.
2001,
Pubmed
Conseil,
Flavonoids: a class of modulators with bifunctional interactions at vicinal ATP- and steroid-binding sites on mouse P-glycoprotein.
1998,
Pubmed
Dhonukshe,
Auxin transport inhibitors impair vesicle motility and actin cytoskeleton dynamics in diverse eukaryotes.
2008,
Pubmed
Fujita,
PIS1, a negative regulator of the action of auxin transport inhibitors in Arabidopsis thaliana.
1997,
Pubmed
Gälweiler,
Regulation of polar auxin transport by AtPIN1 in Arabidopsis vascular tissue.
1998,
Pubmed
Garabagi,
Utility of the P19 suppressor of gene-silencing protein for production of therapeutic antibodies in Nicotiana expression hosts.
2012,
Pubmed
Geisler,
Master and servant: Regulation of auxin transporters by FKBPs and cyclophilins.
2016,
Pubmed
Geisler,
A Critical View on ABC Transporters and Their Interacting Partners in Auxin Transport.
2017,
Pubmed
Geldner,
Auxin transport inhibitors block PIN1 cycling and vesicle trafficking.
2001,
Pubmed
Green,
Quantitative evaluation of the lengths of homobifunctional protein cross-linking reagents used as molecular rulers.
2001,
Pubmed
Gu,
Inhibition of Inositol Polyphosphate Kinases by Quercetin and Related Flavonoids: A Structure-Activity Analysis.
2019,
Pubmed
Gupta,
The role of interfacial lipids in stabilizing membrane protein oligomers.
2017,
Pubmed
Henrichs,
Regulation of ABCB1/PGP1-catalysed auxin transport by linker phosphorylation.
2012,
Pubmed
Kim,
Identification of an ABCB/P-glycoprotein-specific inhibitor of auxin transport by chemical genomics.
2010,
Pubmed
Kourelis,
A homology-guided, genome-based proteome for improved proteomics in the alloploid Nicotiana benthamiana.
2019,
Pubmed
Marquès-Bueno,
A dominant negative mutant of protein kinase CK2 exhibits altered auxin responses in Arabidopsis.
2011,
Pubmed
Mravec,
Interaction of PIN and PGP transport mechanisms in auxin distribution-dependent development.
2008,
Pubmed
Noh,
Multidrug resistance-like genes of Arabidopsis required for auxin transport and auxin-mediated development.
2001,
Pubmed
Okada,
Requirement of the Auxin Polar Transport System in Early Stages of Arabidopsis Floral Bud Formation.
1991,
Pubmed
Opekarová,
Specific lipid requirements of membrane proteins--a putative bottleneck in heterologous expression.
2003,
Pubmed
,
Xenbase
Paciorek,
Auxin inhibits endocytosis and promotes its own efflux from cells.
2005,
Pubmed
Peer,
Mutation of the membrane-associated M1 protease APM1 results in distinct embryonic and seedling developmental defects in Arabidopsis.
2009,
Pubmed
Petrásek,
PIN proteins perform a rate-limiting function in cellular auxin efflux.
2006,
Pubmed
Petrásek,
Do phytotropins inhibit auxin efflux by impairing vesicle traffic?
2003,
Pubmed
Ruegger,
Reduced naphthylphthalamic acid binding in the tir3 mutant of Arabidopsis is associated with a reduction in polar auxin transport and diverse morphological defects.
1997,
Pubmed
Simon,
PIN6 auxin transporter at endoplasmic reticulum and plasma membrane mediates auxin homeostasis and organogenesis in Arabidopsis.
2016,
Pubmed
Skokan,
PIN-driven auxin transport emerged early in streptophyte evolution.
2019,
Pubmed
Sussman,
Solubilization of the receptor for N-1-naphthylphthalamic Acid.
1980,
Pubmed
Sussman,
The action of specific inhibitors of auxin transport on uptake of auxin and binding of N-1-naphthylphthalamic acid to a membrane site in maize coleoptiles.
1981,
Pubmed
Tan,
Salicylic Acid Targets Protein Phosphatase 2A to Attenuate Growth in Plants.
2020,
Pubmed
Teale,
Naphthylphthalamic acid and the mechanism of polar auxin transport.
2018,
Pubmed
Thomson,
1-N-naphthylphthalamic acid and 2,3,5-triiodobenzoic acid : In-vitro binding to particulate cell fractions and action on auxin transport in corn coleoptiles.
1973,
Pubmed
Titapiwatanakun,
ABCB19/PGP19 stabilises PIN1 in membrane microdomains in Arabidopsis.
2009,
Pubmed
Wisniewska,
Polar PIN localization directs auxin flow in plants.
2006,
Pubmed
Wu,
The ER-localized TWD1 immunophilin is necessary for localization of multidrug resistance-like proteins required for polar auxin transport in Arabidopsis roots.
2010,
Pubmed
Yang,
High-affinity auxin transport by the AUX1 influx carrier protein.
2006,
Pubmed
,
Xenbase
Zhang,
Relative Protein Quantification Using Tandem Mass Tag Mass Spectrometry.
2017,
Pubmed
Zhu,
TWISTED DWARF1 Mediates the Action of Auxin Transport Inhibitors on Actin Cytoskeleton Dynamics.
2016,
Pubmed
Zourelidou,
Auxin efflux by PIN-FORMED proteins is activated by two different protein kinases, D6 PROTEIN KINASE and PINOID.
2014,
Pubmed
,
Xenbase
