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Optogenetic Control of the Canonical Wnt Signaling Pathway During Xenopus laevis Embryonic Development.
Krishnamurthy VV
,
Hwang H
,
Fu J
,
Yang J
,
Zhang K
.
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Optogenetics uses light-inducible protein-protein interactions to precisely control the timing, localization, and intensity of signaling activity. The precise spatial and temporal resolution of this emerging technology has proven extremely attractive to the study of embryonic development, a program faithfully replicated to form the same organism from a single cell. We have previously performed a comparative study for optogenetic activation of receptor tyrosine kinases, where we found that the cytoplasm-to-membrane translocation-based optogenetic systems outperform the membrane-anchored dimerization systems in activating the receptor tyrosine kinase signaling in live Xenopus embryos. Here, we determine if this engineering strategy can be generalized to other signaling pathways involving membrane-bound receptors. As a proof of concept, we demonstrate that the cytoplasm-to-membrane translocation of the low-density lipoprotein receptor-related protein-6 (LRP6), a membrane-bound coreceptor for the canonical Wnt pathway, triggers Wnt activity. Optogenetic activation of LRP6 leads to axis duplication in developing Xenopus embryos, indicating that the cytoplasm-to-membrane translocation of the membrane-bound receptor could be a generalizable strategy for the construction of optogenetic systems.
Bergeron,
Nanoparticle-Mediated Upconversion of Near-Infrared Light: A Step Closer to Optogenetic Neuromodulation in Humans.
2018, Pubmed
Bergeron,
Nanoparticle-Mediated Upconversion of Near-Infrared Light: A Step Closer to Optogenetic Neuromodulation in Humans.
2018,
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Birchmeier,
Orchestrating Wnt signalling for metabolic liver zonation.
2016,
Pubmed
Bugaj,
Optogenetic protein clustering and signaling activation in mammalian cells.
2013,
Pubmed
Burke,
Spatiotemporal regulation of liver development by the Wnt/β-catenin pathway.
2018,
Pubmed
Chang,
Light-inducible receptor tyrosine kinases that regulate neurotrophin signalling.
2014,
Pubmed
Chao,
Neurotrophins and their receptors: a convergence point for many signalling pathways.
2003,
Pubmed
Che,
The Dual Characteristics of Light-Induced Cryptochrome 2, Homo-oligomerization and Heterodimerization, for Optogenetic Manipulation in Mammalian Cells.
2015,
Pubmed
Clevers,
Wnt/beta-catenin signaling in development and disease.
2006,
Pubmed
Csanaky,
Membrane-Associated, Not Cytoplasmic or Nuclear, FGFR1 Induces Neuronal Differentiation.
2019,
Pubmed
Day,
Wnt/beta-catenin signaling in mesenchymal progenitors controls osteoblast and chondrocyte differentiation during vertebrate skeletogenesis.
2005,
Pubmed
Duan,
Optical Activation of TrkA Signaling.
2018,
Pubmed
Duan,
Understanding CRY2 interactions for optical control of intracellular signaling.
2017,
Pubmed
Dunty,
Wnt3a/beta-catenin signaling controls posterior body development by coordinating mesoderm formation and segmentation.
2008,
Pubmed
Funayama,
Embryonic axis induction by the armadillo repeat domain of beta-catenin: evidence for intracellular signaling.
1995,
Pubmed
,
Xenbase
Grusch,
Spatio-temporally precise activation of engineered receptor tyrosine kinases by light.
2014,
Pubmed
Hikasa,
Wnt signaling in vertebrate axis specification.
2013,
Pubmed
,
Xenbase
Khamo,
Optogenetic Delineation of Receptor Tyrosine Kinase Subcircuits in PC12 Cell Differentiation.
2019,
Pubmed
Kim,
Spatiotemporal control of fibroblast growth factor receptor signals by blue light.
2014,
Pubmed
Krishnamurthy,
Reversible optogenetic control of kinase activity during differentiation and embryonic development.
2016,
Pubmed
,
Xenbase
Krishnamurthy,
A Generalizable Optogenetic Strategy to Regulate Receptor Tyrosine Kinases during Vertebrate Embryonic Development.
2020,
Pubmed
,
Xenbase
Leopold,
Bacterial Phytochrome as a Scaffold for Engineering of Receptor Tyrosine Kinases Controlled with Near-Infrared Light.
2020,
Pubmed
Leopold,
Neurotrophin receptor tyrosine kinases regulated with near-infrared light.
2019,
Pubmed
Li,
Dkk1 stabilizes Wnt co-receptor LRP6: implication for Wnt ligand-induced LRP6 down-regulation.
2010,
Pubmed
Liang,
Transmembrane protein 198 promotes LRP6 phosphorylation and Wnt signaling activation.
2011,
Pubmed
,
Xenbase
Linker,
beta-Catenin-dependent Wnt signalling controls the epithelial organisation of somites through the activation of paraxis.
2005,
Pubmed
MacDonald,
Frizzled and LRP5/6 receptors for Wnt/β-catenin signaling.
2012,
Pubmed
Machon,
A dynamic gradient of Wnt signaling controls initiation of neurogenesis in the mammalian cortex and cellular specification in the hippocampus.
2007,
Pubmed
Masuda,
Context-dependent regulation of the β-catenin transcriptional complex supports diverse functions of Wnt/β-catenin signaling.
2017,
Pubmed
Metcalfe,
Stability elements in the LRP6 cytoplasmic tail confer efficient signalling upon DIX-dependent polymerization.
2010,
Pubmed
Mohanty,
Optical Techniques in Optogenetics.
2015,
Pubmed
Plouhinec,
Chordin forms a self-organizing morphogen gradient in the extracellular space between ectoderm and mesoderm in the Xenopus embryo.
2013,
Pubmed
,
Xenbase
Rorick,
PP2A:B56epsilon is required for eye induction and eye field separation.
2007,
Pubmed
,
Xenbase
Shah,
CTLA-4 is a direct target of Wnt/beta-catenin signaling and is expressed in human melanoma tumors.
2008,
Pubmed
Stamos,
The β-catenin destruction complex.
2013,
Pubmed
Tamai,
A mechanism for Wnt coreceptor activation.
2004,
Pubmed
,
Xenbase
Taslimi,
Optimized second-generation CRY2-CIB dimerizers and photoactivatable Cre recombinase.
2016,
Pubmed
